HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-mdbk Bos taurus mdbk Madin-Darby bovine kidney cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-fbt cells bovin fetal bovine testicular cells Bovine fbt cells fetal bovine testicular cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-fbt cells bovin fetal bovine testicular cells Bovine fbt cells fetal bovine testicular cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases. The Journal of general virology 2009 imex curation Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation bovin-fbt cells bovin fetal bovine testicular cells Bovine fbt cells fetal bovine testicular cells anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Labiuk SL., Babiuk LA., van Drunen Littel-van den Hurk S. Virus - Publications including interactions involving viral proteins sylvia.vandenhurk@usask.ca Accepted 2016-JAN-28 AT 11:49 GMT AT 11:49 GMT by ORCHARD The Journal of general virology 2009 imex curation ck2b_chlae_protein Casein kinase II subunit beta protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to P67870 q4vw77_9alph UL47 AAADCAAH_00010 BLEONNCJ_00010 BLPDLEPH_00010 DCJDKEDG_00010 DILPLKIK_00010 DJCKHMNK_00010 NBBNDGNH_00010 NCKHNGOI_00010 NFOBEAPH_00010 OCMKPLLD_00010 OHMFJBFK_00010 protein protein Bovine herpesvirus 1 Bovine herpesvirus 1 MDAARDGRPERRPRRSGTYRTHPFQRPSARRSLLDALRAADAEAAERPRVRRPRPDFQRPPDEDTSEDENVYDYIDGDSSDSADDYDSDYFTANRGPNHGAGDAMDTDAPPERAPEGGAPQDYLTAHLRAIEALPESAPHRSLLERTARTVYAQQFPPRDLSAGSRAPAQRARRSLRGFPRGGGGGQEPGPDDEGDDAADLREDLVPDEAYAHLERDERLSEGPPLLNMEAAAAAAGERSVVEELFTYAPAQPQVEVPLPRILEGRVRPSAFFAQMPLDALCRTPPNDQRVVRERRAWEMAGTPHGLLITTWSTVDPEFSIGGMYVGAPEGTRPRLVWRRAMKQAMALQYRLGVGGLCRAVDGAAMPPTEALLFLADALLRVVRNCHFLSRPGRAGGAARRLPAAAVGLLAATQFTPPDASPHATLFRGSMGSLIYWHELRVMLTAVPALCARYAGAGLQSAELYLLALAHSEAPGYTANERYALSAYLTLFVALAERALRWLYLAGAHLLGPHPTAAAFREVRAKIPYERLPLGSATLHDAEVETVDSATFQEALAFSALAHVYGEAYVAVRTATTLLMAEYAAHAERRDVREMTAAFLGVGLIAQRLMGSLNLLLNCVAGAAVYGGRRVTVREGTLARYSLLADAALPLVRPVSLVEFWEARDGVMRELRLRPVASPPLAGKRRVMELYLSLDSIEALVGREPLGSRPVLGPLVDIAEALADHPHLVTGDGRGPRLGGR 3A04260F20E877EC csk2b_bovin Casein kinase II subunit beta CSNK2B CK2N Phosvitin protein protein bovin Bos taurus (Bovine) Bovine MSSSEEVSWISWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRGIAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSPVKTIR E465B1E699B0E0EC actb_bovin Actin, cytoplasmic 1 ACTB Beta-actin protein protein bovin Bos taurus (Bovine) Bovine MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P60712 original sequence version: 105 6AFD05CA94E360E2 o39503_9alph US3 protein protein Bovine herpesvirus type 1.1 Bovine herpesvirus type 1.1 MERAAERLARQRARGLWRSRFACCVAAEPSGEPARPERSRSRCGSARCAAAGSADLYLAVNNEGPEVAPPARTGPPDADGIEGGAAVGNEQGGVAAGNERRAAIGDKKKSASGGENESESESESESESGSESGADDGDWDDDDDAGPAGGVTREEAEGAARALNFRIIRRLTPGSEGRVFEATGPAPAQEHVVLKIGASASTLAEAMLLRTLDHANVVKLKAVLFHGELVCVVLARYREDLHTHLWRINRPLALPAALAVTRAVLRGLAYLHSRRIAHRDVKTENVFLNGPGDVCLGDFGAAHGPVTEPRYYGLAGTLETNSPELLARARYDCRTDVWSAGVVAYEMLAYPRALFDSPAGPQGEDAEASGPPTILGDRDCARQLLRVIRRLAVHAEEFPPSPTDRLTRNFKRHASTRREPHSPYRCLAVLRLPCDADRLLHQMLTFDFRARPTAAELLEHPVFGAASG 1DB78931F87274C9 ddb1_bovin DNA damage-binding protein 1 DDB1 Damage-specific DNA-binding protein 1 protein protein bovin Bos taurus (Bovine) Bovine MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGMSPLCAIGLWTDISARIAKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH 8E4A2A0859180271 o39498_9alph UL1 protein protein Bovine herpesvirus type 1.1 Bovine herpesvirus type 1.1 MAPAVARACAWALLAALLWLPPAGATRRADSAESILAERCRGNLLLADRPQHEEAAPGLAGIFIRGRCSPPEAALWYEDTGETYWANPYAVARGLAEDIRRVLADTPVYRDLAIQVLNSAFGLPHEVRAPLPPPPRGCVLPPRYHTTGPCGPGDGIYR D9694E671CD0403D q06635_9alph BICP4 protein protein Bovine herpesvirus 1 Bovine herpesvirus 1 MSSPGRLELATLDLYDLIESADLGPTGGSEEDPALLDAARRAEARERRRAARAELAELWRMVGGEDAESSEDDGAGDAGATEGAEAEDAEIGEDAGARDAGGAEDADVAECAEAEGAEGAEDADSAWAAARALAVAVEAAAAGAEAAAAEAAVIAAAIEAEAGAAPLKAEAGTEAGGGGALGARTEAGDVGPEDCSDAGSEDSGPEDCSDAGPEDDGGEAREGSTDADAEAEAEAEAGTGAGTRTKAAPREAGAGPGAPRGRAPGPPAPSPPETAPPPPRRQRGRATGARAGYAAAPRDGPPPLEGPLLTPSGEAWPGSAPPPPGRVRFGGAGDTREGLWDCPEIREAAARYAAAAGPAAVFVPEMGDAGKQYAALVDLVYARRDAMAWLQSAKLAGPDLQLARLLQRRVQGCRGHSSFITGSVTAPLPPVGDAMAAQNALWALPHVAACVAMSRRYDCDQKLFLLQSLRRAYAPMAYPEAGAGGSGARAALAELRAVLAGRAAPAPLPPASTARAARERLRELADRCAVACREALEAARRAAGAAGLPVLSAAAGRGLPAAACAPDALAAHPERVLRAAELLGAARDAVERARLQRAAPAALRAEAAAALEAAALAARTVAPLARYSTRGAAARASAWALARALFSPPAEVPARLAAALAALEAAGGGAGAGAAAPGRGPVEVEVEDMRAGAPRADPEDGSEAEDGEDGEDEEAEEEEEEEKEEEEEEEEEEEEEEEGRPGPAAGAPPGPREGRRAEEEEEEEEEEEGDGDGDGEAAALPAAAGGPSGEDDSGPRRRRKAAAADAAGPARREPPLPARVLGPMPPGGPAADGGFRRVPPGDYHTPAPSAAALAAYCRPEVAARLADHPLFPEPWRPALAFDPEALAEIAARRRAGPAGRSPPPLRRRVAWMSQIADPEDVRVVVLYDPLPGEALAAPPGEDERRRPEWPPRRGGLSHALAALGNRLLLSADSHAWAGRWTGAPDVSALGAQGVLLLSTRDLAFRGAVEYLCARLAAARRRLIVLDAVDPENWPRDGPAVGQAHVYLRAAVLPRRSAPRAGPSGALARAVLASRRVFGPGAFARAEAAYARLYPEAPPLRLCRGGNVRYTVATRLGPRTAVPVPPREYRQRVLPALDGRKDMAAQGAALGLGEPDFVEGEAASHRAANRWGLGAPLRPVYLACGRRALELAPDELPAAAVAFCAAALPEPRAEAPPLVLEAAAAPPAAAAPGVDWDADQGPRETLVLLRRAAGGGLVERVPPPAAEAPPARVPAQSSPGAGSGFPAPAGGGRRPRRPRRGPPPRVEVLSSSSSSSSSSSSAASSPASSDDDEAGAAGGGPASP 9A89EB1A8CFA37B5 ul47-multi-1 6 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association 1, S1 and S2 etif3 is also identified by MALDI-TOF vp8-us3-2 2 11 unspecified role unspecified role bait bait c teminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey physical association physical association 3A ul47-us3-1 4 11 unspecified role unspecified role prey prey c terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3B ul47-us3-2 1 11 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 4A ul47-us3-3 3 11 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 4B us3-ul47-1 4 11 unspecified role unspecified role bait bait c terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3B ul47-ck2b-1 5 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3C