IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Journal of virology 2009 imex curation Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus schultz@hwi.buffalo.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Accepted 2020-DEC-08 AT 08:24 GMT AT 08:24 GMT by ORCHARD Journal of virology 2009 Only protein-protein interactions imex curation imex curation Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Journal of virology 2009 imex curation Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus schultz@hwi.buffalo.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Accepted 2020-DEC-08 AT 08:24 GMT AT 08:24 GMT by ORCHARD Journal of virology 2009 Only protein-protein interactions imex curation imex curation Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Severe acute respiratory syndrome coronavirus nsp9 dimerization is essential for efficient viral growth. Journal of virology 2009 imex curation Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus schultz@hwi.buffalo.edu 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Miknis ZJ., Donaldson EF., Umland TC., Rimmer RA., Baric RS., Schultz LW. Virus - Publications including interactions involving viral proteins schultz@hwi.buffalo.edu Accepted 2020-DEC-08 AT 08:24 GMT AT 08:24 GMT by ORCHARD Journal of virology 2009 Only protein-protein interactions imex curation imex curation p0c6u8-pro_0000338263 Non-structural protein 9 ORF1a polyprotein 1a protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus NNELSPVALRQMSCAAGTTQTACTDDNALAYYNNSKGGRFVLALLSDHQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ 4118 4230 EC021D1E6D6297A0 pro_0000338263-1 2 4 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6U8-PRO_0000338263:p.Gly104Glu mutation mutation certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 19:59:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly104Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 2.6Å Rwork: 0.216 Rfree: 0.268 pro_0000338263-2 1 4 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6U8-PRO_0000338263:p.Gly104Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 16:45:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly104Glu' P0C6U8-PRO_0000338263:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:15:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly100Glu' P0C6U8-PRO_0000338263:p.Gly104Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G V Tue Jun 30 17:55:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly104Val' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2 Dimerization occurs at protein concentrations between 40 and 105 M // A weak dimerization constant of 0.871 mM was determined for G104V pro_0000338263-4 3 4 unspecified role unspecified role neutral component neutral component purified purified c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0C6U8-PRO_0000338263:p.Gly104Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:13:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly104Glu' P0C6U8-PRO_0000338263:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 19:56:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly100Glu' P0C6U8-PRO_0000338263:p.Gly104Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G V Tue Jun 30 18:22:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly104Val' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2 Dimerization occurs at protein concentrations between 40 and 105 M // A weak dimerization constant of 0.871 mM was determined for G104V