HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD Human thymus cDNA J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD Human thymus cDNA J. Virol. (0022-538X) 2007 imex curation An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J. Virol. (0022-538X) 2007 imex curation Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Kim C.S., Seol S.K., Song O.K., Park J.H., Jang S.K. Virus - Publications including interactions involving viral proteins HCV isolate H is used as the isolate could not be traced Interactions involving transfected hnRNPA1 could not be curated as the species of origin of the construct could not be traced sungkey@postech.ac.kr Accepted 2014-MAY-01 by ORCHARD J. Virol. (0022-538X) 2007 imex curation sept6_human Septin-6 SEPTIN6 KIAA0128 SEP2 SEPT6 protein protein human Homo sapiens Human MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE DIP protein Q14141 original sequence version: 120 DIP protein Q14141 original sequence version: 152 DIP protein Q14141 original sequence version: 128 E684AE6A93B32362 sept2_human Septin-2 SEPTIN2 DIFF6 KIAA0158 NEDD5 Neural precursor cell expressed developmentally down-regulated protein 5 SEPT2 protein protein human Homo sapiens Human MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV 12CCBBE30806F92F 12CCBBE30806F92F p27958-pro_0000037577 RNA-directed RNA polymerase protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) SMSYSWTGALVTPCAAEEQKLPINALSNSLLRHHNLVYSTTSRSACQRKKKVTFDRLQVLDSHYQDVLKEVKAAASKVKANLLSVEEACSLAPPHSAKSKFGYGAKDVRCHARKAVAHINSVWKDLLEDSVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSKLPLAVMGSSYGFQYSPGQRVEFLVQAWKSKKTPMGLSYDTRCFDSTVTESDIRTEEAIYQCCDLDPQARVAIKSLTERLYVGGPLTNSRGENCGYRRCRASRVLTTSCGNTLTRYIKARAACRAAGLQDCTMLVCGDDLVVICESAGVQEDAASLRAFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDGAGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMFAPTLWARMILMTHFFSVLIARDQLEQALNCEIYGACYSIEPLDLPPIIQRLHGLSAFSLHSYSPGEINRVAACLRKLGVPPLRAWRHRAWSVRARLLARGGKAAICGKYLFNWAVRTKLKLTPITAAGRLDLSGWFTAGYSGGDIYHSVSHARPRWFWFCLLLLAAGVGIYLLPNR 2421 3011 2F89B19E29A81A02 roa1_human Heterogeneous nuclear ribonucleoprotein A1 HNRNPA1 HNRPA1 Helix-destabilizing protein Single-strand RNA-binding protein hnRNP core protein A1 protein protein human Homo sapiens Human MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF DIP protein P09651 original sequence version: 176 A06683571C6C109F rl6_human 60S ribosomal protein L6 RPL6 TXREB1 Tax-responsive enhancer element-binding protein 107 Neoplasm-related protein C140 Large ribosomal subunit protein eL6 protein protein human Homo sapiens Human MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF D24A6168C7D0ECBF if4a2_human Eukaryotic initiation factor 4A-II ATP-dependent RNA helicase eIF4A-2 EIF4A2 DDX2B EIF4F protein protein human Homo sapiens Human MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI DIP protein Q14240 original sequence version: null FAA7D3BA9D8C6DA0 rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN 070EB4C904E6795A ns5b-multi-2 7 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 1A and C ns5b-hnrnpa1-1 5 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 1B and D hnrnpa1-septin6-1 5 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait egfp egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3Cii hnrnpa1-septin6-ns5b-septin2-1 1 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Fig. 3D hnrnpa1-ns5b-1 1 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 1E p27958_pro-hnrnpa1-1 3 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2 ns5b-hnrnpa1-2 4 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association Results ns5b-hnrnpa1-3 6 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait physical association physical association 3B ns5b-hnrnpa1-4 2 10 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey egfp egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3Bii sept6-hnrnpa1-septin2-1 1 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Fig. 3D ns5a-if4a2-1 4 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Results