IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT human lymphocyte cDNA library Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system. Journal of medical virology 2006 imex curation Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus gaof@im.ac.cn 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Fan Z., Zhuo Y., Tan X., Zhou Z., Yuan J., Qiang B., Yan J., Peng X., Gao GF. Virus - Publications including interactions involving viral proteins gaof@im.ac.cn Accepted 2020-APR-22 AT 07:21 BST AT 07:21 BST by MGT Journal of medical virology 2006 Only protein-protein interactions imex curation imex curation ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63279 original sequence version: 33 DIP protein P63279 original sequence version: 57 DIP protein P63279 original sequence version: 80 DIP protein P63279 original sequence version: 137 DIP protein P63279 original sequence version: 99 DIP protein P63279 original sequence version: 140 DIP protein P63279 original sequence version: 120 DIP protein P63279 original sequence version: 117 DIP protein P63279 original sequence version: 93 E2C826E9C8D0683D ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 n-ube2i-5 1 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association n-ube2i-6 3 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association Fig. 2a, 6b n-ube2i-7 4 9 unspecified role unspecified role prey prey cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 3a ube2i-n-1 5 9 unspecified role unspecified role prey prey cell lysate cell lysate infection infection chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell epithelium Epithelium [epithelial cells] 8 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 3b n-ube2i-8 2 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4 n-ube2i-9 6 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell c-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell c-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 5 ube2i-n-2 5 9 unspecified role unspecified role prey prey cell lysate cell lysate infection infection P59595:p.Lys62Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:24:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys62Ala' 8 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 6a ube2i-n-3 7 9 unspecified role unspecified role prey prey cell lysate cell lysate infection infection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role bait bait purified purified P63279:p.Cys93Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 17:36:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ala' P63279:p.Cys93Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C S Tue Jun 30 18:20:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ser' n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 7