HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. Virology 2004 imex curation Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down autoradiography autoradiography Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-MAY-26 AT 12:07 BST AT 12:07 BST by MGT Virology 2004 imex curation The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. Virology 2004 imex curation Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-MAY-26 AT 12:07 BST AT 12:07 BST by MGT Virology 2004 imex curation The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. The equine herpesvirus 1 EICP27 protein enhances gene expression via an interaction with TATA box-binding protein. Virology 2004 imex curation Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip autoradiography autoradiography Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ., Albrecht RA., Kim SK., Zhang Y., Zhao Y., O'Callaghan DJ. Virus - Publications including interactions involving viral proteins docall@lsuhsc.edu Accepted 2016-MAY-26 AT 12:07 BST AT 12:07 BST by MGT Virology 2004 imex curation tf2b_human Transcription initiation factor IIB GTF2B TF2B TFIIB General transcription factor TFIIB S300-II protein protein human Homo sapiens Human MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL DIP protein Q00403 original sequence version: 119 DIP protein Q00403 original sequence version: 181 9CC7E102526C2722 tbp_human TATA-box-binding protein TBP TF2D TFIID GTF2D1 TATA-box factor TATA-binding factor TATA sequence-binding protein Transcription initiation factor TFIID TBP subunit protein protein human Homo sapiens Human MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT DIP protein P20226 original sequence version: 106 DIP protein P20226 original sequence version: 157 DIP protein P20226 original sequence version: 173 DIP protein P20226 original sequence version: 194 DIP protein P20226 original sequence version: 179 DIP protein P20226 original sequence version: 131 A61A578D972B970B icp27_ehv1k mRNA export factor ICP27 homolog Transcriptional regulator IE63 homolog UL3 protein protein ehv1k Equine herpesvirus 1 (strain Kentucky A) EHV-1 Equine abortion virus MALSSVSSCEPMEDEMSIMGSDTEDNFTGGDTCAEATRGLVNKSAFVPTQTVGTVSALRNVVGNPPKSVVVSFSASPQRAQPSNPKSERPAFGHGRRNRRRPFRRNNWKQQQRGWEKPEPENVPARQSAGSWPKRSSLPVHMRLGQRGGDSSSADSGHGGAGPSDRWRFKTRTQSVARVHRNRRRGNANHGSNTPGRSAGDRLNAAAARSIADVCRRVTSSRIGEMFHGARETLTTPVKNGGFRAENSSPWAPVLGFGSDQFNPEARRITWDTLVEHGVNLYKLFEVRSHAAEAARSLRDAVMRGENLLEALASADETLSWCKMIVTKNLPMRTRDPIISSSVALLDNLRLKLEPFMRCYLSSSGSPTLAELCDHQRLSDVACVPTFMFVMLARIARAVGSGAETVSRDALGPDGRVLADYVPGACLAGTLEAIDAHKRRCKADTCSLVSAYTLVPVYLHGKYFYCNQIF 48DBE440E11363F7 icp27-tbp-1 1 5 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 6A icp27_ehv1k-tbp-1 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 6B eicp27-tbp-2 3 5 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6C tfiib-tbp-2 3 5 unspecified role unspecified role bait bait 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 6C eicp27-tbp-1 1 5 unspecified role unspecified role bait bait n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey 35s label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 7