MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Mouse alpha1-syntrophin binding to Grb2: further evidence of a role for syntrophin in cell signaling. Biochemistry (0006-2960) 2001 rapid curation Oak SA., Russo K., Petrucci TC., Jarrett HW. Virus - Publications including interactions involving viral proteins no in vitro In vitro pull down pull down predetermined predetermined participant predetermined Oak SA., Russo K., Petrucci TC., Jarrett HW. Biochemistry (0006-2960) 2001 rapid curation snta1_rabit Alpha-1-syntrophin SNTA1 SNT1 59-1 DAP Syntrophin-1 59 kDa dystrophin-associated protein A1 acidic component 1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MASGRRAPRTGLLELRAGTGAGAGGERWQRVLVSLAEDALTVSPADGEPGPEPGAVREPEPAQINGAAEPGAAPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQALKKTGKEVVLEVKYMKEVSPYFKNSAGGTSVGWDSPPASPLQRQPSSPGPQTRNLSEAKHVPLKMAYVSRRCTPSDPEHRYLEICSADGQDTIFLRAKDEASARSWAGAIQAQINALLPWVKDELQALLAASSPAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYGGLPQTREALSRPARTAPLIATRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRPCNLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTMVFIIHSFLSAKVTRLGLLA mint Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate 5E4151266C0DD1AB snta1_mouse Alpha-1-syntrophin Snta1 Snt1 59 kDa dystrophin-associated protein A1 acidic component 1 Syntrophin-1 protein protein mouse Mus musculus Mouse MASGRRAPRTGLLELRCGAGSGAGGERWQRVLLSLAEDALTVSPADGEPGPEPEPAQLNGAAEPGAAPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNGEDLSSATHDEAVQALKKTGKEVVLEVKYMKEVSPYFKNSAGGTSVGWDSPPASPLQRQPSSPGPQPRNLSEAKHVSLKMAYVSRRCTPTDPEPRYLEICAADGQDAVFLRAKDEASARSWAGAIQAQIGTFIPWVKDELQALLTATGTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLFYCSLPQSREALSRPTRTAPLIATSSAHRLVHSGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGIQEVSTACTWNGRPCSLSVHIDKGFTLWAAEPGAARAMLLRQPFEKLQMSSDDGTSLLFLDFGGAEGEIQLDLHSCPKTMVFIIHSFLSAKVTRLGLLA DIP protein Q61234 original sequence version: 86 161BA76CAF50AE96 g1sr27_rabit GRB2 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 q28685-pro_0000021070 Beta-dystroglycan DAG1 Dystrophin-associated glycoprotein 1 Dystroglycan protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit SIVVEWTNNTLPLEPCPKEQITGLSRRIAEDNGQPRPAFTNALEPDFKATSIAITGSGSCRHLQFIPVAPPGIPSSVTPPTEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPSQSVPETTPLNQDTVGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP 654 895 EE7E8EE3E10F10F6 grb2_mouse Growth factor receptor-bound protein 2 Grb2 Adapter protein GRB2 SH2/SH3 adapter GRB2 protein protein mouse Mus musculus Mouse MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 97F4A4FE4B248DDF grb2-snta1-5 2 SNTA1 8 unspecified role unspecified role prey prey purified purified Stoichiometry: 1.0 GRB2 11 unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis n-term range n-terminal range n-term range n-term range n-terminal range n-term range SH3 DOMAIN Stoichiometry: 1.0 direct interaction direct interaction Fig. 6 snta1-grb2 1 GRB2 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 SNTA1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction homomint mint Fig. 7 snta1-grb2-3 3 SNTA1 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 9 unspecified role unspecified role prey prey physical association physical association Fig. 4 grb2-snta1-3 5 GRB2 11 unspecified role unspecified role bait bait purified purified Stoichiometry: 1.0 SNTA1 8 unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain PROLINE RICH range MINT-586512: original sequence: 172-274 : NSAGGTSVGWDSPPASPLQRQPSSPGPQPRNLSEAKHVSLKMAYVSRRCTPTDPEPRYLEICAADGQDAVFLRAKDEASARSWAGAIQAQIGTFIPWVKDEL Stoichiometry: 1.0 direct interaction direct interaction homomint mint grb2-dag1-1 6 7 unspecified role unspecified role prey prey rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 10 unspecified role unspecified role prey prey rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle GRB2 11 unspecified role unspecified role bait bait purified purified Stoichiometry: 1.0 direct interaction direct interaction homomint mint Fig. 2 dag1-grb2-1 4 SNTA1 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1