HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase. Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase. The Journal of biological chemistry 1999 imex curation Thome M., Martinon F., Hofmann K., Rubio V., Steiner V., Schneider P., Mattmann C., Tschopp J. Virus - Publications including interactions involving viral proteins jurg.tschopp@ib.unil.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Thome M., Martinon F., Hofmann K., Rubio V., Steiner V., Schneider P., Mattmann C., Tschopp J. Virus - Publications including interactions involving viral proteins jurg.tschopp@ib.unil.ch Accepted 2016-JUN-15 AT 11:59 BST AT 11:59 BST by MGT The Journal of biological chemistry 1999 imex curation Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase. Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase. The Journal of biological chemistry 1999 imex curation Thome M., Martinon F., Hofmann K., Rubio V., Steiner V., Schneider P., Mattmann C., Tschopp J. Virus - Publications including interactions involving viral proteins jurg.tschopp@ib.unil.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Thome M., Martinon F., Hofmann K., Rubio V., Steiner V., Schneider P., Mattmann C., Tschopp J. Virus - Publications including interactions involving viral proteins Interactions in figure 5c and 5d have not been added since the origins/species of the TRAF proteins could not be traced. jurg.tschopp@ib.unil.ch Accepted 2016-JUN-15 AT 11:59 BST AT 11:59 BST by MGT The Journal of biological chemistry 1999 imex curation bcl10_mouse B-cell lymphoma/leukemia 10 Bcl10 Ciper Clap B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein protein protein mouse Mus musculus Mouse MEAPAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPRGLDTLVESIRREKTQSFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFAAGATNNLSRCNSDESNLSEKQRASTVMYHPEGESSTAPFFSMASSLNLPVLEVGRTENSSFSSATLPRPGDPGAPPLPPDLRLEEGGSCGNSSEMFLPLRSRALSRQ DIP protein Q9Z0H7 original sequence version: 100 mint homomint Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex C0539BC97102DBB8 vclap_ehv2 CARD domain-containing protein E10 E10 protein protein EHV-2 Equine herpesvirus 2 (strain 86/87) MAEKYPLQAGDPCVTLTEEDIWDVERLCLEELRVLLVSHLKSHKHLDHLRAKKILSREDAEEVSSRATSRSRAGLLVDMCQDHPRGFQCLKESCKNEVGQEHLVDLLERAFEKHCGDKLTQKWWESGADGRNPRRPPGSEDNSGYTALLPTNPSGGGPSIGSGHSRPRGRDDSGGIGGGVYPFSHGGARVVGGGWGGWGESGGAGRGGSLLSGGHGGHPPHGGPGGGGRDYYGGGGSGYYESIPEPANFPNSGGGGRGGGVRYDAGGDGRLGGLPPDPQEVDDPSLSVQGRGGPAPDPPSPPLRTRRFFCC 04EC68C7353D723B bcl10_human B-cell lymphoma/leukemia 10 BCL10 CIPER CLAP Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B protein protein human Homo sapiens Human MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ DIP protein O95999 original sequence version: 69 DIP protein O95999 original sequence version: 122 DIP protein O95999 original sequence version: 60 DIP protein O95999 original sequence version: 86 F87C97F2B784BA4B v_carmen-h_carmen-1 2 5 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait card region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4A & B h_carmen-h_carmen-1 2 5 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait card region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4B m_carmen-m_carmen-1 2 3 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4B v_carmen-m_carmen-1 2 3 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait card region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4B v_carmen-h_carmen-2 1 5 unspecified role unspecified role prey prey card region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association table 1 v_carmen-v_carmen-1 1 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey card region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association table 1