HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro triphosphatase ass nucleoside triphosphatase assay triphosphatase ass autoradiography autoradiography You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... ATP or dATP used as substrate The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT J. Virol. (0022-538X) 1999 imex curation Hepatitis C virus core protein interacts with cellular putative RNA helicase. Hepatitis C virus core protein interacts with cellular putative RNA helicase. J. Virol. (0022-538X) 1999 imex curation You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ym.edu.tw 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence You L.R., Chen C.M., Yeh T.S., Tsai T.Y., Mai R.T., Lin C.H., Lee Y.H. Virus - Publications including interactions involving viral proteins I have corrected all the errors you did not, but Expt-7 is too much....I have added new comments under the old comment (which is in brackets) - but this experiment will take some time to complete.... The protein CAP-Rf has been unambiguously identified as ATP-dependent RNA helicase DDX3X (O00571) by blasting the N-terminus of the sequence given in Figure 1 - which shows a protein of 661 amino acids. However the sequence length of the current and previous versions of UniProt for this protein shows a 662 amino acid protein. Thus constructs including this protein all are shown with an indeterminate length. yhwulee@ ym.edu.tw yhwulee@ym.edu.tw Accepted 2014-AUG-28 by MGT Human liver cDNA J. Virol. (0022-538X) 1999 imex curation gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 ddx3x_human ATP-dependent RNA helicase DDX3X DDX3X DBX DDX3 DEAD box protein 3, X-chromosomal Helicase-like protein 2 DEAD box, X isoform CAP-Rf protein protein human Homo sapiens Human MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN DIP protein O00571 original sequence version: 116 DIP protein O00571 original sequence version: 123 7074D2B8A6EBBF09 utp UTP 5'-UTP H4utp Uridine 5'-triphosphate uridine 5'-triphosphoric acid Uridine triphosphate UTP uridine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) PGAVKCOVUIYSFO-XVFCMESISA-N PGAVKCOVUIYSFO-XVFCMESISA-N dgtp dGTP 2'-deoxyguanosine 5'-triphosphate 2'-Deoxyguanosine 5'-triphosphate Deoxyguanosine 5'-triphosphate Deoxyguanosine triphosphate dGTP 2'-deoxyguanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) HAAZLUGHYHWQIW-KVQBGUIXSA-N HAAZLUGHYHWQIW-KVQBGUIXSA-N p27958-pro_0000037566 Core protein precursor protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) STNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRPEGRTWAQPGYPWPLYGNEGCGWAGWLLSPRGSRPSWGPTDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTVPASA 2 191 7C9059C0D1D2732E dutp dUTP 2'-deoxyuridine 5'-triphosphate 2'-Deoxyuridine 5'-triphosphate deoxyuridine triphosphate dUTP 2'-deoxyuridine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) AHCYMLUZIRLXAA-SHYZEUOFSA-N AHCYMLUZIRLXAA-SHYZEUOFSA-N atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ctp CTP 5'-CTP CTP Cytidine 5'-triphosphate Cytidine triphosphate CYTIDINE-5'-TRIPHOSPHATE H4ctp cytidine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) PCDQPRRSZKQHHS-XVFCMESISA-N PCDQPRRSZKQHHS-XVFCMESISA-N dctp dCTP 2'-Deoxycytidine 5'-triphosphate dCTP Deoxycytidine 5'-triphosphate Deoxycytidine triphosphate 2'-deoxycytidine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) RGWHQCVHVJXOKC-SHYZEUOFSA-N RGWHQCVHVJXOKC-SHYZEUOFSA-N datp dATP 2'-deoxyadenosine 5'-triphosphate 2'-Deoxyadenosine 5'-triphosphate dATP Deoxyadenosine 5'-triphosphate Deoxyadenosine triphosphate 2'-deoxyadenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) SUYVUBYJARFZHO-RRKCRQDMSA-N SUYVUBYJARFZHO-RRKCRQDMSA-N core-caprf-6 7 12 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association no figure Positive results were obtained using truncated versions of core proteins as bait, but not when using the full-length version. core-caprf-1 3 12 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 4B His CAP-Rd could associate with the truncated versions (C122 and C101) of in vitro-translated HCV core protein but not with the full-length one (C195) core-caprf-2 6 9 unspecified role unspecified role prey prey human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 4C core-caprf-3 1 9 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 core-caprf-4 4 9 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 6 core-caprf-5 5 12 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind range range range range physical association physical association no figure Positive results were obtained using truncated versions of core proteins as bait, but not when using the full-length version. ddx3x-p27958_pro-1 1 12 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role neutral component neutral component n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 5C and D atp-ddx3x-2 2 12 stimulator stimulator ancillary ancillary region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 14 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) atpase reaction atpase reaction 7, 8 and table 2 polynucleotide A, U, G, C and dI-dC datp-ddx3x-1 2 17 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 12 stimulator stimulator ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7, 8 and table 2 polynucleotide A, U, G, C and dI-dC ddx3x-utp-1 2 10 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC ddx3x-dutp-1 2 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 13 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC ddx3x-gtp-1 2 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 8 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) gtpase reaction gtpase reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC ddx3x-dgtp-1 2 11 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC ctp-ddx3x-1 2 15 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC dctp-ddx3x-1 2 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). 16 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7 and table 2 polynucleotide A, U, G, C and dI-dC datp-ddx3x-2 2 17 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 7, 8 and table 2 polynucleotide A, U, G, C and dI-dC atp-ddx3x-1 2 14 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 enzyme enzyme neutral component neutral component n terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). atpase reaction atpase reaction 7, 8 and table 2 polynucleotide A, U, G, C and dI-dC