IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. (0021-9258) 1997 Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 1997 SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. (0021-9258) 1997 Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. in vitro In vitro protein array protein array predetermined predetermined participant predetermined Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 1997 SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. (0021-9258) 1997 Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. COS cell extract was used but the authors do not specify which strain. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 1997 SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. (0021-9258) 1997 Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. imaging technique imaging technique microscopy immunostaining immunostaining Takeuchi M., Hata Y., Hirao K., Toyoda A., Irie M., Takai Y. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 1997 dlg1_drome Disks large 1 tumor suppressor protein dlg1 l(1)dlg1 CG1725 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPVKKQEAHRALELLEDYHARLSEPQDRALRIAIERVIRIFKSRLFQALLDIQEFYELTLLDDSKSIQQKTAETLQIATKWEKDGQAVKIADFIKSSNLNRNCAYEFNNDASSNQTNQSALNQNPIANNVSAQAQAEALSRTFKSELEEILNQRMRIESDTENAKEPTVEQQQKQQQAQQRSSRSPQQQNPQQQQGSKSRSGSQTVNGDDSWLYEDIQLERGNSGLGFSIAGGTDNPHIGTDTSIYITKLISGGAAAADGRLSINDIIVSVNDVSVVDVPHASAVDALKKAGNVVKLHVKRKRGTATTPAAGSAAGDARDSAASGPKVIEIDLVKGGKGLGFSIAGGIGNQHIPGDNGIYVTKLMDGGAAQVDGRLSIGDKLIAVRTNGSEKNLENVTHELAVATLKSITDKVTLIIGKTQHLTTSASGGGGGGLSSGQQLSQSQSQLATSQSQSQVHQQQHATPMVNSQSTEPGSRYASTNVLAAVPPGTPRAVSTEDITREPRTITIQKGPQGLGFNIVGGEDGQGIYVSFILAGGPADLGSELKRGDQLLSVNNVNLTHATHEEAAQALKTSGGVVTLLAQYRPEEYNRFEARIQELKQQAALGAGGSGTLLRTTQKRSLYVRALFDYDPNRDDGLPSRGLPFKHGDILHVTNASDDEWWQARRVLGDNEDEQIGIVPSKRRWERKMRARDRSVKFQGHAAANNNLDKQSTLDRKKKNFTFSRKFPFMKSRDEKNEDGSDQEPFMLCYTQDDANAEGASEENVLSYEAVQRLSINYTRPVIILGPLKDRINDDLISEYPDKFGSCVPHTTRPKREYEVDGRDYHFVSSREQMERDIQNHLFIEAGQYNDNLYGTSVASVREVAEKGKHCILDVSGNAIKRLQVAQLYPVAVFIKPKSVDSVMEMNRRMTEEQAKKTYERAIKMEQEFGEYFTGVVQGDTIEEIYSKVKSMIWSQSGPTIWVPSKESL DIP protein P31007 original sequence version: 157 544D1E1AD03B0674 dlgp4_rat Disks large-associated protein 4 Dlgap4 Sapap4 SAP90/PSD-95-associated protein 4 PSD-95/SAP90-binding protein 4 Dap4 protein protein rat Rattus norvegicus (Rat) Rat MKGLGDSRPRHLSDSLDPPHEPLFAGPDRNPYLLSPTEAFAREARFPGQNTLPGDGLFPLNNQLPPPSSTFPRIHYNSHFEVPEESPFPSHAQATKINRLPANLLDQFEKQLPIHRDGFSTLQFPRGEAKARGESPGRIRHLVHSVQRLFFTKAPSMEGTTGKVGGNGGKKGVLEDGKGRRAKSKERAKAGEPKRRSRSNISGWWSSDDNLDGEGGAFRSGPASGLMTLGRQPERTQPRYFMHAYNTISGHMLKTTKNTTTELTAPPPPPAPPATCPSLGVGTDTNYVKRGSWSTLTLSHAHEVCQKTSATLDKSLLKSKSCHQGLAYHYLQVPGGGGEWSTTLLSPRDMDSTAEGPIPCRRMRSGSYIKAMGDEDSDESGGGSPKPSPKTAARRQSYLRATQQSLGEQSNPRRSLDRLDSVDMPLPSKYPSWEEDYNPISDSLNDSGCISQVFGQASLIPQLFGHDQQVREADLSDQYEAACESACSEAESTAAEALDLSLPSYFRSRSHSYLRAIQAGCSQEEDSVSLQSLSPPPSTGSLSNSRTLPSSSCLVAYKKTPPPVPPRTTSKPFISVTVQSSTESAQDTYLDSQDHKSEVTSQSGLSNSSDSLDSSTRPPSVTRGGITPGPEAPEPPPKHAALKSEHGTLTSSESHSEAVPKRKLSSIGIQVDCIQPVPKEEPSPATKFQSIGVQVEDDWRSSAPSHSMSSRRDTDSDTQDANDSSCKSSERSLPDCTSHPNSISIDAGPRQAPKIAQIKRNLSYGDNSDPALEASSLPPPDPWMETSSSSPAEPAQPGACRRDGYWFLKLLQAETERLEGWCCQMDKETKENNLSEEVLGKVLSAVGSAQLLMSQKFQQFRGICEQNLNPDANPRPTAQDLAGFWDLLQLSIEDISMKFDELYHLKANSWQLVETPEKRKEEKKPPPPVPKKPAKSKAAVSRDKASDAGDKQRQEARKRLLAAKRAASVRQNSATESADSIEIYVPEAQTRL 8875B67C3FD71F04 dlg1_rat Disks large homolog 1 Dlg1 Synapse-associated protein 97 Dlgh1 protein protein rat Rattus norvegicus (Rat) Rat MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESPVVLPSTPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKAFRKNHEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKAAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLRETMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYHEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL DIP protein Q62696 original sequence version: 129 DIP protein Q62696 original sequence version: 155 18CEBD31DD0CAF8B dlgp1_rat Disks large-associated protein 1 Dlgap1 Gkap Guanylate kinase-associated protein SAP90/PSD-95-associated protein 1 PSD-95/SAP90-binding protein 1 protein protein rat Rattus norvegicus (Rat) Rat MKGLSGSRSHHHGITCESACDSLSHHSDHKPYLLSPVDHHPADHPYYTQRNSFQAECVGPFSDPLASSTFPRRHYTSQQELKDESALVPRTLATKANRLPTNLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRHLVHSVQKLFTKSHSLEGASKGGVNGGKASPDGSQTVRYGKRSKSKERRSEPKARSNASNASPTSPSWWSSDDNLDGDMCLYHTPSGVMTMGRCPDRSVSQYFMGAYNTISEQAVKASRSNNDVKCSTCANLPVTLDAPLLKKSAWSSTLTVSRAREVYQKASVNMDQAVVKSEACQQERSCQYLQVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTGVIKLSSAVEVSSCITTYKKTPPPVPPRTTTKPFISITAQSSTESAQDAYMDGQGQRGDMISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGSNAAAVTTTTTIATVTTEDRKKDFKKNRCLSIGIQVDDAEESEKMAESKTSSKFQSVGVQVEEEKCFRRFTRSNSVTTAVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCKQMEREERENNLPEDILGKIRTAVGSAQLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAESIEIYIPEAQTRL 44BAF9BC0C14C099 dlg3_rat Disks large homolog 3 Dlg3 Dlgh3 Synapse-associated protein 102 PSD-95/SAP90-related protein 1 protein protein rat Rattus norvegicus (Rat) Rat MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKNTPKLNGSGPSWWPECTCTNRDWYEQASPAPLLVNPEALEPSLSVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL 34DA9C46C7BB96DB dlgp3_rat Disks large-associated protein 3 Dlgap3 SAP90/PSD-95-associated protein 3 PSD-95/SAP90-binding protein 3 Dap3 protein protein rat Rattus norvegicus (Rat) Rat MRGYHGDRGSHPRPARFADQQHMDVGPAARAPYLLGSREAFSTEPRFCAPRAGLGHLSPEGPLSLSEGPSSVGPEGGPGGVGAGGSSSTFPRMYPGQGPFDTCEDCVGHPQGKGATRLLPTFLDQFEKQLPVQQDGFHTLPYQRGPAGPGPGPGSGAAPEARSESPSRIRHLVHSVQKLFAKSHSLEAPGKRDYNGPKAEGRSSSGGDSYSGPGSGGPPTSHHHHHHHHHHHHQSRHGKRSKSKDRKGDGRHQTKATGWWSSDDNLDSDSGFLGGRPPGEPGGPFCLDAPDGSYRDLSFKGRSGGSEGRCLACTGMSMSLDGQSVKRSAWHTMMVSQGRDGYPGAGPGKGLLGPETKAKARTYHYLQVPQDDWGGYPTGGKDGEIPCRRMRSGSYIKAMGDEESGDSDGSPKTSPKALARRFASRRSSSVDTARINCCVPPRIHPRSSIPGYSRSLTTGQLSEEFNQQLEAVCGSVFGELESQAVDALDLPGCFRMRSHSYLRAIQAGCSQDDDCLPLLAAPASVSGRPGSSFNFRKAPPPIPPGSQAPPRISITAQSSTDSAHESFTAAEGPARRCSSADGLDGPTMGARTLELAPVPPRASPKPPTLIIKTIPGREELRSLARQRKWRPSIGVQVETISDSDTENRSRREFHSIGVQVEEDKRRARFKRSNSVTAGVQADLELEGLAGLATVATEDKALQFGRPFQRQASEPQPGPRAPTYSVFRTVHTQGQWAYREGYPLPYEPPATDGSPGPPPVPAPGPGSGRRDSWMERGSRSLPDSGRTSPCPRDGEWFIKMLRAEVEKLEHWCQQMEREAEDYELPEEILEKIRSAVGSTQLLLSQKVQQFFRLCQQSLDPTAFPVPTFQDLAGFWDLLQLSIEDVTLKFLELQQLKANSWKLLEPKEEKKVPPPIPKKPSRGRGVPVKERSLDSVDRQRQEARKRLLAAKRAASFRHSSATESADSIEIYIPEAQTRL AD65DB11E1D4F723 dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein P31016 original sequence version: 86 DIP protein P31016-1 original sequence version: 148 7922D4E8E0F9AD85 dlgp2_rat Disks large-associated protein 2 Dlgap2 Dap2 SAP90/PSD-95-associated protein 2 PSD-95/SAP90-binding protein 2 protein protein rat Rattus norvegicus (Rat) Rat MGTAQVLPGILQKHCCILPDRNTESQCTLCGEPEEEEGGDLAQPGLSFPGPAEEDIDQQYSWSPTQHFSEERYSPAPRNMKGLTGSRNQPQLCVGHTCGLSPTDECEHPHDHVRHGPDVRQPYLLSPAESCPMDHHRCSPRSSVHSECMMMPVMLGDHVSSSTFPRMHYSSHYDTRDDCATSHASTKVNRIPANLLDQFEKQLPLHRDGFHTLQYHRASAATEQRNESPGRIRHLVHSVQKLFTKSHSLEGSSKSNINGTKSEGRMDDHHQSHLSKHSKRSKSKERKPESKHKSGMSSWWSSDDNLDSDSTYRTPSVAHRHHMDHIPHCYPEALQSPFGDLSLKTSKSNSDVKCSACEGLALTPDTRYMKRSSWSTLTVSQAKEAYRKSSLNLDKPLVHPEIKPSLQPCHYLQVPQDDWGAYPTGGKEEEIPCRRMRSGSYIKAMGDEESGESDSSPKTSPTVALRPEPLLKSIIQRPLGDHQTQSYLQAATEVPVGHSLDPSVNYNSPKFRSRNQSYMRAVSTLSQASCVSQMSEAEVNGQFESVCESVFSEVESQAMDALDLPGCFRTRSHSYLRAIQAGYSQDDECIPVMTPSNMTSTIRSTAAVSYTNYKKTPPPVPPRTTSKPLISVTAQSSTESTQDAYQDSRAQRMSPWPQDSRGGLYNSMDSLDSNKAMNLALESAAAQRHAADTQSSSTRSIDKAVLVSKAEELLKSRCSSIGVQDSEFPDHQPYPRSDVETATDSDTESRGLREYHSVGVQVEDEKRHGRFKRSNSVTAAVQADLELEGFPGHVSMEDKGLQFGSSFQRHSEPSTPTQYGALRTVRTQGLFSYREDYRTQVDTSTLPPPDPWLEPSLDTVETGRMSPCRRDGSWFLKLLHTETKKMEGWCKEMEREAEENDLSEEILGKIRSAVGSAQLLMSQKFQQFYWLCQQNMDPSAMPRPTSQDLAGYWDMLQLSVEDVSMKFDELHQLKLNDWKIMESPERKEERKIPPPIPKKPPKGKFPITREKSLDLPDRQRQEARRRLMAAKRAASFRQNSATERADSIEIYIPEAQTRL 8AB2A3667AD79434 dlg4-dlgap1-1 1 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 430-724 of P31036 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of P31016 (amino acids 534-724). dlg4-dlgap2-1 1 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 430-724 of P31036 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of P31016 (amino acids 534-724). dlg4-dlgap3-1 1 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 430-724 of P31036 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of P31016 (amino acids 534-724). dlg4-dlgap4-1 1 11 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 430-724 of P31036 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of P31016 (amino acids 534-724). dlg1-dlg4-1 1 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 587-910 of Q62696 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62696(amino acids 726-910). dlg1-dlgap3-1 1 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 587-910 of Q62696 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62696(amino acids 726-910). dlg1-dlgap2-1 1 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 587-910 of Q62696 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62696(amino acids 726-910). dlg1-dlgap1-1 1 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 587-910 of Q62696 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62696(amino acids 726-910). dlg3-dlgap1-1 1 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 526-835 of Q62936 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62936 (amino acids 645-835). dlg3-dlgap2-1 1 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 526-835 of Q62936 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62936 (amino acids 645-835). dlg3-dlgap3-1 1 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 526-835 of Q62936 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62936 (amino acids 645-835). dlg3-dlgap4-1 1 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated to occur using both of the following baits:1.Amino acids 526-835 of Q62936 containing the SH3 and guanylate kinase domains.2.The guanylate kinase domain of Q62936 (amino acids 645-835). dlgap1-dlg1-1 1 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated with both of the following baits:1.Amino acids 601-960 of P31007 containing the SH3 and guanylate kinase domains. 2. The guanylate kinase domain of P31007 (amino acids 770-960) dlgap1-dlgap2-1 1 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role prey prey physical association physical association This interaction was demonstrated with both of the following baits:1.Amino acids 601-960 of P31007 containing the SH3 and guanylate kinase domains. 2. The guanylate kinase domain of P31007 (amino acids 770-960) dlg1-dlgap3-2 1 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated with both of the following baits:1.Amino acids 601-960 of P31007 containing the SH3 and guanylate kinase domains. 2. The guanylate kinase domain of P31007 (amino acids 770-960) dlg1-dlgap4-1 1 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association This interaction was demonstrated with both of the following baits:1.Amino acids 601-960 of P31007 containing the SH3 and guanylate kinase domains. 2. The guanylate kinase domain of P31007 (amino acids 770-960) dlg4-dlgap1-2 3 11 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association PSD95 was full length and GST tagged. Revisit when protein editor in place. The authors demonstrated that the SH3 and guanylate kinase domains of PSD95 (P31016: amino acids 430-724) are sufficient for this interaction. dlgap1-dlg4-1 2 8 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Revisit when protein editor in place. Bait was GST tagged.Prey was the guanylate kinase domain(amino acids 534-724)of PSD95. Dissociation constant was 2.1x10-7M. dlgap1-dlg4-2 4 8 unspecified role unspecified role neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. colocalization colocalization The guanylate kinase domain of PSD95 is sufficient for this interaction. This interaction was shown to take place at the plasma membrane. dlgap2-dlg4-2 2 11 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction Revisit when protein editor in place. Bait was GST tagged.Prey was the guanylate kinase domain(amino acids 534-724)of PSD95. Dissociation constant was 2.3x10-7M.