IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-p12 human p12 p12 Cell line established from the peripheral blood of a 7-year-old boy with acute lymphoblastic leukemia bifunctional agent crosslink protein cross-linking with a bifunctional reagent Label transfer techniques Photoaffinity labelling Label transfer techniques Photoaffinity labelling bifunctional agent crosslink predetermined predetermined participant predetermined Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 chromatography chromatography technology column chromatography chromatography partial id prot seq partial identification of protein sequence partial id prot seq Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 competition binding competition binding predetermined predetermined participant predetermined Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer The binding of TGFB2 to betaAPP is shown as an absence of a band on the blot, since excess TGFB2 competed out binding of radiolabelled TGFB1. Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Transforming growth factor-beta bound to soluble derivatives of the beta amyloid precursor protein of Alzheimer's disease. Biochem. Biophys. Res. Commun. (0006-291X) 1990 Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Cancer - Interactions investigated in the context of cancer Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Apoptosis - Interactions involving proteins with a function related to apoptosis Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bodmer S., Podlisny MB., Selkoe DJ., Heid I., Fontana A. Cancer - Interactions investigated in the context of cancer Sequential chromatography as follows: hydroxylapatite, reversed-phase Pro-RPC (TFA-acetonitrile), cation-exchange on Mono-S and final reversed-phase Pro-RPC (TFA-2propanol). 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. Biochem. Biophys. Res. Commun. (0006-291X) 1990 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 tgfb2_human Transforming growth factor beta-2 proprotein Glioblastoma-derived T-cell suppressor factor Cetermin TGFB2 protein protein human Homo sapiens Human MHYCVLSAFLILHLVTVALSLSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPEEVPPEVISIYNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPPFFPSENAIPPTFYRPYFRIVRFDVSAMEKNASNLVKAEFRVFRLQNPKARVPEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYTSGDQKTIKSTRKKNSGKTPHLLLMLLPSYRLESQQTNRRKKRALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS 7D9D569E0F4A07D0 tgfb1_human Transforming growth factor beta-1 proprotein TGFB1 TGFB protein protein human Homo sapiens Human MPPSGLRLLLLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS DIP protein P01137 original sequence version: 149 DIP protein P01137 original sequence version: 185 DIP protein P01137 original sequence version: 160 75391614250288FE tgfb2-bapp-1 2 7 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 8 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 physical association physical association tgfb2-bapp-2 5 7 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 8 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 physical association physical association tgfb2-bapp-3 6 7 unspecified role unspecified role prey prey human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 8 unspecified role unspecified role bait bait human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 physical association physical association tgfb2-bapp-4 4 7 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 8 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 physical association physical association tgfb1-bapp-1 1 7 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 9 unspecified role unspecified role neutral component neutral component 125i Iodine 125 isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association tgfb2-bapp-5 1 7 unspecified role unspecified role neutral component neutral component human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 8 unspecified role unspecified role neutral component neutral component in vitro In vitro physical association physical association tgfb1-tgfb2-bapp-2 3 7 unspecified role unspecified role bait bait human-308 Human cystic glioblastoma cell line 308 308 Human cystic glioblastoma cell line 308 9 unspecified role unspecified role prey prey 125i Iodine 125 isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 inhibitor inhibitor prey prey in vitro In vitro association association