IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions caeel-coelomocyte Caenorhabditis elegans coelomyocyte coelomocyte Coelomocyte Large cells full of vesicles that are situated in the pseudocoelomic space of the worms. Scavenger cells that continuously and nonspecifically endocytose fluid from the pseudocoelom (body cavity). fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. 13-Sept-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. 13-Sept-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. 13-Sept-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. Fluorescently labelled proteins visualised by tag fluorescence. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. 13-Sept-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Identification of the switch in early-to-late endosome transition. Identification of the switch in early-to-late endosome transition. Cell (0092-8674) 2010 imex curation Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch 13-Sept-2010: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay western blot western blot Immuno blot Immuno blot Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Accepted 2010-JUL-30 by JYOTI. 13-Sept-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi5p 1-phosphatidyl-1D-myo-inositol 5-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q8i100_caeel rabx-5 CELE_Y39A1A.5 Y39A1A.5 protein protein caeel Caenorhabditis elegans MSDKHGEEDKKAKMHLKANDLLCVNGCGFYGTPQWENRCSKCWRAHQNEMKKCQDFAKNRSLLSFDQFQERRKSTTESKSRGIKNLFKTSPIPEGGTSSPTSTSPASTPTRRRELSPDSLEARQQFTDFLVANLSTGMAQEIARSVKNAVNKISEMRMSSDDMSELVMSYYQYLGERIGGHSLFDSPDCKVKVEDVMDQVEKYISTFCYSIFFCANHEEEVADMSLQDRIRSLHWVTAGFLETKMVFKKQTVRDKIDEAISELIEINAKRSAFEKLDCLTKSCKAIFEALKESEASTSADEFLPTLIYVLFRGNPPLIQSNVKFISRFAIPARLMSGEAAYFFTNLSCALEFARNMNHESLQMEKSEFEAYTSGHLAPPLSVINSACNQAIYVLEGTIETITNVAKKAGSLAKNLTNMHGKSDDDLEKMLAVIKETVDFYPTDEYMNMKQSIFAEEKETADILVSLSRQSSESGRGTLTDTQPSTPQPDTSLESPLIAEIQAINLTSSGNQEHVEEA B884B6A67CD06CED q9xvl8_caeel ccz-1 F58G11.6 CELE_F58G11.6 protein protein caeel Caenorhabditis elegans MESIANPLYFFSNLSLNPPSCSNVVRFIDVLEFFFVAHPLSGRKEGEEHNRVMYFHPKGEQLERQTEITGFAEAVVNFTENFLSTSRREISERISENEDGFDFRTVTTQRTEHVYIRTEDDQFILGVSISKQLSLVSDYPLFQPAIRSILSDAYKMFRMFFGTFSSFIKNVPDDIPKFKERLDFFFSKYIPLLKVHKMPLLDHLGGVEFLRMSGPLYLNVVSLLSELREEFQVIEKIMFLYQDKLLYYQLSKRDLPSLFHYLTHNLLPTTLAPELEHSGRNASKGRYLRGPTDLTTDAPLLGDESLSVVHLHSDQGTEFQEELIGYQMIVYRCLNATVCMFVRNCPANGGLEIRRDSESSQSSRATPSQNGVVSRRLLRNIDQFLETELSQVASKIGDEIGDEKFPEAPDFHYIYFNPSSLSMTSSLSTSPKLPVAASGSNSTLKVPLPPVDVNRLVCDTMSNFVSETEEFGECFVKSSSDWWIVIKKVNSRLLVLIIPPSNYTSSLADVQSKTATIVRSHFEAIFFS 715740C995F46E39 vps16_human Vacuolar protein sorting-associated protein 16 homolog VPS16 protein protein human Homo sapiens Human MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK 9C4292D455C19A60 q23146_caeel rab-7 CELE_W03C9.3 W03C9.3 protein protein caeel Caenorhabditis elegans MSGTRKKALLKVIILGDSGVGKTSLMNQYVNRRFSNQYKATIGADFLTRDVNIDDRTVTLQIWDTAGQERFQSLGVAFYRGADCCVLAFDVTNAASFKSLDSWRDEFLIQASPRDPDHFPFVLLGNKVDLESQRAVSSKRAQSWCQTKGNIPYYEVSAKEALNVEAAFLAIARDALARESQETNDFPEFPDQIRLNPNQQNQQNSGCNC 1797B3E7B887F255 pi4p 1-phosphatidyl-1D-myo-inositol 4-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mon1b_human Vacuolar fusion protein MON1 homolog B MON1B HSRG1 KIAA0872 SAND2 HSV-I stimulating-related protein HSV-1 stimulation-related gene 1 protein protein protein human Homo sapiens Human MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEETGSKDKDQPPSPSPPPQSEALSSTSRLWSPAAPENSPTCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTSQSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTGL 06ED0B7C42AA2D17 mon1a_human Vacuolar fusion protein MON1 homolog A MON1A SAND1 protein protein human Homo sapiens Human MHPGGGPSRAERLELGLGRERPAKAIFLHRRPGEGGGRERCLRCGHVCVRRGPGPREAVPSGRPRPDTLTPPWVRQRAVTGTFCASWTPLRNRRAQRMATDMQRKRSSECLDGTLTPSDGQSMERAESPTPGMAQGMEPGAGQEGAMFVHARSYEDLTESEDGAASGDSHKEGTRGPPPLPTDMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWLEPPGAVGRPATEPPREGTTEGDEEDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHADGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLLSGSERITDNLLQLMARDPSFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSILLARNQLVALVRRKDQFLHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFHAHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALRTPYYSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLHSRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFILTPLTY 344D326577E66FE2 q9bi89_caeel Vacuolar fusion protein MON1 homolog sand-1 F41H10.11 CELE_F41H10.11 protein protein caeel Caenorhabditis elegans MEVEVPGGSEAADTGSEASYKVSQVEIEDVEENLKNADVIFEHLEQLPFQVFILSEFGKPIFVNNDRNEGEIVSLVALICAFVSRCQSWGDSLMTMTSQDNHIQFLHKSPLIFCVVSKYPEQLDQQLEVLFEQICSILSKSQLENVYKKKGDNYDLRKLLRGTDRLIDSSISSWRASPINLVDSSISAIPMNPSDREFLSTTMASCLGAAKLDGALFGIMIARRQIAAMVRFKKYMIHPRDLNIVINLVSDNTLQTDSQNWVPICLPRFNDTGFFYAYISYPWCNKEQDIPVCIVLLSVKRDHFDGLKEVRQQIVTKLENNQKFFVNFAQAMKTPNLYQISQIGSNSESLWSFLYLNHSSKQVCMSASKIPLITRDERWIARSEMRRSSSLSSLHPHLRTLFVRGTKHCLFVWVTDLFSLYCIFGPFVTATIAFQVVEKLLKSLKSHEQRYFIINSTSF E1479678C8B6E716 vps11_human Vacuolar protein sorting-associated protein 11 homolog VPS11 PP3476 RING finger protein 108 RNF108 protein protein human Homo sapiens Human MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLRCSNDSFSVIADYFGRGVFNKLTLLTDPPTARLTSSLEAGLQRDLLMHSRRGT C1F8E538ED1B56DB vps18_human Vacuolar protein sorting-associated protein 18 homolog VPS18 KIAA1475 protein protein human Homo sapiens Human MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL 0008E21D66E8CFEC p91857_caeel rab-5 CELE_F26H9.6 F26H9.6 protein protein caeel Caenorhabditis elegans MAARNAGTARPGGPNRTCQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDATIKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQESFQKAKNWVKELQRQASPNIVMALAGNKADVANKRTVEYEEANAYAEDNALLFMETSAKTSMNVNDIFMAIAKKLPIGPAQGEPTGTVDMNQPQQQQKGSCCK 3DCD9DA1716AB992 vp33a_human Vacuolar protein sorting-associated protein 33A VPS33A protein protein human Homo sapiens Human MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGNRLPAADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMKREFTGSQNSIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLIFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLMNGTSWIEALMEKPF 12442A1D9A223F56 vps41_human Vacuolar protein sorting-associated protein 41 homolog VPS41 S53 protein protein human Homo sapiens Human MAEAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCKQFVTGGKKLLLFERSWMNRWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKVCSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRDYHLEYSEGESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK 037577D5FA3A18A0 rabx5-mon1b-1 4 14 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association The mammalian SAND-1 homolog Mon1b interacts with Rabex5 in vitro. Fig. 2B, Supp. Fig. 2E rabx5-rab5 4 19 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 2B rabx5-sand1 2 16 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 2B and that His6-RABX-5 bound to GST-SAND-1 (Figure S2B). Figure legend appears to be incorrect. Nucleotide ->amino acid conversion given in text refering to Sup. Fig. 5 rabx5-sand1-1 3 16 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2A, Supp. Fig. 2C Schematic drawing depicting the SAND-1 interaction with the helical bundle domain of RABX-5 in yeast two-hybrid assay. rabx5-mon1b-2 5 9 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Interestingly, a subpopulation of Mon1a/b colocalized with Rabex5 on some endosomal structures. Fig. 4E pi3p-sand1-1 6 16 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait direct interaction direct interaction SAND-1 recognized monophosphorylated phosphoinositides, most strongly PI(3)P (Figure 6C). Fig. 6C, Supp. Fig. 5 pi4p-sand1-2 6 16 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait direct interaction direct interaction Phospholipid strips were used to determine the phospholipid binding site in SAND-1 Fig. 6C, Supp. Fig. 5 pi5p-sand1-1 6 8 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Phospholipid strips were used to determine the phospholipid binding site in SAND-1 Fig. 6C, Supp. Fig. 5 sand1-rab7 1 12 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization The panel shows time frames of coelomocyte expressing GFP::SAND-1 and mCherry::RAB-7. Figs. 6B, 7A Wortmannin mon1b-ccz1 3 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association The known interaction between SAND-1 and CCZ-1 (Poteryaev et al., 2007) Fig. 7D mon1b-vps11-1 3 17 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association Yeast two-hybrid interaction between Mon1b and Vps 11 and Vps18. Fig. 7D mon1b-vps18-1 3 14 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey physical association physical association Yeast two-hybrid interaction between Mon1b and Vps 11 and Vps18. Fig. 7D mon1b-vps16-1 2 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 7F Moreover, recombinant HOPS complex members Vps16A, Vps18, Vps33A, and Vps41 bound to Mon1b-GST in vitro (Figure 7F) mon1b-vps18-2 2 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 18 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 7F Moreover, recombinant HOPS complex members Vps16A, Vps18, Vps33A, and Vps41 bound to Mon1b-GST in vitro (Figure 7F) mon1b-vps33a-1 2 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 7F Moreover, recombinant HOPS complex members Vps16A, Vps18, Vps33A, and Vps41 bound to Mon1b-GST in vitro (Figure 7F) mon1b-vps41-1 2 21 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 7F Moreover, recombinant HOPS complex members Vps16A, Vps18, Vps33A, and Vps41 bound to Mon1b-GST in vitro (Figure 7F) mon1a-vps33a-1 2 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 7F Moreover, recombinant HOPS complex members Vps16A, Vps18, Vps33A, and Vps41 bound to Mon1b-GST in vitro (Figure 7F) sand1-ccz1-1 3 10 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association The known interaction between SAND-1 and CCZ-1 (Poteryaev et al., 2007) Supp. Fig. 2C rabx5-mon1a-1 4 15 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Recombinant His6-Mon1a and His6-Mon1b interact directly with GST-Rabex-5 in vitro. Supp. Fig. 2E