IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. Cell (0092-8674) 2010 imex curation Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. ylu1@lsuhsc.edu 25-Jun-2010: Contacted by JYOTI. jyoti Only protein-protein interactions 9 mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis ylu1@lsuhsc.edu Accepted 2010-MAR-26 by JYOTI. 25-Jun-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions 9 imex curation DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. Cell (0092-8674) 2010 imex curation Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. ylu1@lsuhsc.edu 25-Jun-2010: Contacted by JYOTI. jyoti Only protein-protein interactions 9 mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis ylu1@lsuhsc.edu Accepted 2010-MAR-26 by JYOTI. 25-Jun-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions 9 imex curation DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. Cell (0092-8674) 2010 imex curation Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. ylu1@lsuhsc.edu 25-Jun-2010: Contacted by JYOTI. jyoti Only protein-protein interactions 9 mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis ylu1@lsuhsc.edu Accepted 2010-MAR-26 by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions 9 imex curation DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. DAPK1 Interaction with NMDA Receptor NR2B Subunits Mediates Brain Damage in Stroke. Cell (0092-8674) 2010 imex curation Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. ylu1@lsuhsc.edu 25-Jun-2010: Contacted by JYOTI. jyoti Only protein-protein interactions 9 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tu W., Xu X., Peng L., Zhong X., Zhang W., Soundarapandian MM., Balel C., Wang M., Jia N., Zhang W., Lew F., Chan SL., Chen Y., Lu Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of expression constructs supplied by the author. Details of expression systems are not available. Data from Fig. S1 not added as source of NR1 not available. ylu1@lsuhsc.edu Accepted 2010-MAR-26 by JYOTI. 25-Jun-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions 9 imex curation dapk1_mouse Death-associated protein kinase 1 Dapk1 protein protein mouse Mus musculus Mouse MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWASRHGHVDTLKFLNENKCPLDVKDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGYYSVAKALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVIKTLLGHGSFVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGANVEALTSDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKSTLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLAAKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYDKDTSLLKEIRNRFGNDLHVSNKLFVLDAGASGSKDIKVLRNHLQEIRSQIVSGCSPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEDDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTMEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDIPALIKTDSLQRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSAEGDADIRLWVSGCRIANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIETVMATTLPGLLTVKHYLSPQQLREHHEPVMVYQPRDFFRAQTLKESSLTNTMGGYKESFSSITCFGCHDVYSQASLGMDIHASDLSLLTRRKLSRLLDPPDPMGKDWCLLAMNLGLPDMVAKHNVNNRASRDFLPSPVHALLQEWTSYPESTVGILISKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSRRDSHAWTPLYDL 243A14D7C6598F63 nmde2_mouse Glutamate receptor ionotropic, NMDA 2B Glutamate [NMDA] receptor subunit epsilon-2 Grin2b N-methyl D-aspartate receptor subtype 2B protein protein mouse Mus musculus Mouse MKPSAECCSPKFWLVLAVLAVSGSKARSQKSAPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVLADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLISVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSTSSIDGLYDCDNPPFTTQPRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYSGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHLKHGTGDKHGVVGGVPAPWEKNLTNVDWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVSSNASTTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMPAGESSFANKSSVTTAGHHHNNPGSGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGASKTRPDFRALVTNKPVVSALHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV DIP protein Q01097 original sequence version: 113 DIP protein Q01097 original sequence version: 112 DIP protein Q01097 original sequence version: 135 B8C3FA10E9A4B36D dlg4_mouse Disks large homolog 4 Dlg4 Dlgh4 Psd95 Postsynaptic density protein 95 Synapse-associated protein 90 protein protein mouse Mus musculus Mouse MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKIIEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFHFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein Q62108 original sequence version: 84 DIP protein Q62108 original sequence version: 110 DIP protein Q62108 original sequence version: 109 DIP protein Q62108 original sequence version: 133 7EFFC99E1FFF90BA nmdz1_mouse Glutamate receptor ionotropic, NMDA 1 Glutamate [NMDA] receptor subunit zeta-1 Grin1 Glurz1 N-methyl-D-aspartate receptor subunit NR1 protein protein mouse Mus musculus Mouse MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARDLEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCVDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES DIP protein P35438 original sequence version: 115 DIP protein P35438 original sequence version: 114 DIP protein P35438 original sequence version: 122 C610632DD3E06171 kcc2a_mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha Camk2a protein protein mouse Mus musculus Mouse MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKNDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH DIP protein P11798 original sequence version: 97 306F416CCE9B5F62 grin2b-dapk1-1 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Interaction observed in mice operated on to induce ischemia. Figs. 1 and 1B grin2b-dapk1-2 1 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Interaction observed in mice operated on to induce ischemia. Fig. 1C grin2b-grin1-1 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Interaction observed in non-ischemic mice. Fig. 1C grin2b-dapk1-3 1 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey physical association physical association Interaction observed in mice operated on to induce ischemia. Fig. 1C grin2b-dapk1-4 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Figs. 1E and 1F Single neuronal labeling demonstrated a colocalization of DAPK1 with NMDA receptor NR2B subunits grin2b-dapk1-5 4 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined catalytic region sufficient to bind sufficient binding region sufficient to bind range range range range 32 36 direct interaction direct interaction Affinity binding assay shows that flag-tagged catalytic domain of DAPK1 binds with GST-NR2B839-1482 (∼100 kDa) and GST-NR2B1292-1304 (∼30 kDa). The blots show anti-Flag (top) and anti-GST (bottom). Figs. 2A, 2B grin2b-dapk1-6 4 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 5 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] association association Affinity binding assay shows that flag-tagged catalytic domain of DAPK1 binds with GST-NR2B839-1482 (∼100 kDa) and GST-NR2B1292-1304 (∼30 kDa). The blots show anti-Flag (top) and anti-GST (bottom). Figs. 2A, 2B grin2b-dapk1-7 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Membrane extracts were precipitated with nonspecific IgG (IgG) or anti-NR2B in the presence of 50 mM NR2BCT or sNR2BCT and probed with anti-DAPK1, or anti-PSD-95, or anti-CaMK-IIa. Fig. 2D When immunoprecipitation were performed in the presence of Nr2b C-terminal peptide, the interaction with Dak1 but not Dlg4 or Camk2a was disrupted. grin2b-dapk1-8 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Anti-NR2B precipitates were blotted with anti-DAPK1. Supp. Fig. 2E NMDA/glycine - activates endogenous DAPK1.