IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell (0092-8674) 2007 imex curation Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu Accepted 2007-NOV-18 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 5 imex curation Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell (0092-8674) 2007 imex curation Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu Accepted 2007-NOV-18 by DAVET. 13-Nov-2007: Contacted by JYOTI. Cell (0092-8674) 2007 davet Only protein-protein interactions 5 imex curation Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell (0092-8674) 2007 imex curation Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 5 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kremer BE., Adang LA., Macara IG. Synapse - Interactions of proteins with an established role in the presynapse. Data from Supp. Fig. 3 not added as anti-NCK AB recognises both NCK1 and NCK2 igm9c@virginia.edu Accepted 2007-NOV-18 by DAVET. Cell (0092-8674) 2007 davet Only protein-protein interactions 5 imex curation sept6_human Septin-6 SEPTIN6 KIAA0128 SEP2 SEPT6 protein protein human Homo sapiens Human MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE DIP protein Q14141 original sequence version: 120 DIP protein Q14141 original sequence version: 152 DIP protein Q14141 original sequence version: 128 E684AE6A93B32362 o14512-1 Suppressor of cytokine signaling 7 SOCS7 NAP4 SOCS6 Nck, Ash and phospholipase C gamma-binding protein Nck-associated protein 4 protein protein human Homo sapiens Human MVFRNVGRPPEEEDVEAAPEPGPSELLCPRHRCALDPKALPPGLALERTWGPAAGLEAQLAALGLGQPAGPGVKTVGGGCCPCPCPPQPPPPQPQPPAAAPQAGEDPTETSDALLVLEGLESEAESLETNSCSEEELSSPGRGGGGGGRLLLQPPGPELPPVPFPLQDLVPLGRLSRGEQQQQQQQQPPPPPPPPGPLRPLAGPSRKGSFKIRLSRLFRTKSCNGGSGGGDGTGKRPSGELAASAASLTDMGGSAGRELDAGRKPKLTRTQSAFSPVSFSPLFTGETVSLVDVDISQRGLTSPHPPTPPPPPRRSLSLLDDISGTLPTSVLVAPMGSSLQSFPLPPPPPPHAPDAFPRIAPIRAAESLHSQPPQHLQCPLYRPDSSSFAASLRELEKCGWYWGPMNWEDAEMKLKGKPDGSFLVRDSSDPRYILSLSFRSQGITHHTRMEHYRGTFSLWCHPKFEDRCQSVVEFIKRAIMHSKNGKFLYFLRSRVPGLPPTPVQLLYPVSRFSNVKSLQHLCRFRIRQLVRIDHIPDLPLPKPLISYIRKFYYYDPQEEVYLSLKEAQLISKQKQEVEPST 3F326532262AF6AA sept2_human Septin-2 SEPTIN2 DIFF6 KIAA0158 NEDD5 Neural precursor cell expressed developmentally down-regulated protein 5 SEPT2 protein protein human Homo sapiens Human MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV 12CCBBE30806F92F 12CCBBE30806F92F socs7_human Suppressor of cytokine signaling 7 SOCS7 NAP4 SOCS6 Nck, Ash and phospholipase C gamma-binding protein Nck-associated protein 4 protein protein human Homo sapiens Human MVFRNVGRPPEEEDVEAAPEPGPSELLCPRHRCALDPKALPPGLALERTWGPAAGLEAQLAALGLGQPAGPGVKTVGGGCCPCPCPPQPPPPQPQPPAAAPQAGEDPTETSDALLVLEGLESEAESLETNSCSEEELSSPGRGGGGGGRLLLQPPGPELPPVPFPLQDLVPLGRLSRGEQQQQQQQQPPPPPPPPGPLRPLAGPSRKGSFKIRLSRLFRTKSCNGGSGGGDGTGKRPSGELAASAASLTDMGGSAGRELDAGRKPKLTRTQSAFSPVSFSPLFTGETVSLVDVDISQRGLTSPHPPTPPPPPRRSLSLLDDISGTLPTSVLVAPMGSSLQSFPLPPPPPPHAPDAFPRIAPIRAAESLHSQPPQHLQCPLYRPDSSSFAASLRELEKCGWYWGPMNWEDAEMKLKGKPDGSFLVRDSSDPRYILSLSFRSQGITHHTRMEHYRGTFSLWCHPKFEDRCQSVVEFIKRAIMHSKNGKFLYFLRSRVPGLPPTPVQLLYPVSRFSNVKSLQHLCRFRIRQLVRIDHIPDLPLPKPLISYIRKFYYYDPQEEVYLSLKEAQLISKQKQEVEPST 3F326532262AF6AA sept6-socs7-1 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association endogenous SEPT6 was immunoprecipitated from HeLa cell lysate, and the precipitates were probed for endogenous SOCS7 and SEPT2. Fig. 4A From the size of the coimmunoprecipitated SOCS7 protein and further analysis (see text for details) the authors concluded that "HeLa cells express an unusually long splice variant of SOCS7". The SOCS7 protein identified in this experiment had a significantly larger size (Daltons), as determined from SDS PAGE analysis, than the protein identified by the UniProt accession number O14512. sept6-sept2-1 3 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 4A Sept2 and Sept6 interact in cells depleted in SOCS7 sept6-socs7-2 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Septins interact with longer-variant SOCS7, but not with the splice variant NAP4. Fig. 4B sept6-socs7-4 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey association association Additionally, the isolated N-terminus of SOCS7 fused to GFP (GFP-SOCS7(1�124)) coprecipitated with the septin complex (Figure 4C). Fig. 4C