DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2007 imex curation Wang B., Yang L., Wang Z., Zheng H. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Species of origin of APLP1 and APLP2 supplied by author. huiz@bcm.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang B., Yang L., Wang Z., Zheng H. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of origin of APLP1 and APLP2 supplied by author. huiz@bcm.edu Accepted 2008-DEC-19 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2007 jyoti IMEx aplp2_human Amyloid beta precursor like protein 2 APPH APPL2 APLP2 Amyloid protein homolog CDEI box-binding protein Amyloid beta (A4) precursor-like protein 2 Amyloid-like protein 2 Sperm membrane protein YWK-II protein protein human Homo sapiens Human MAATGTAAAAATGRLLLLLLVGLTAPALALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEIPPFHPFHPFPALPENEDTQPELYHPMKKGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNAERVGGLEEERESVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI DIP protein Q06481 original sequence version: 94 CA3A7D6DDB8A28D0 sc5a7_mouse High affinity choline transporter 1 Slc5a7 Cht1 Hemicholinium-3-sensitive choline transporter Solute carrier family 5 member 7 protein protein mouse Mus musculus Mouse MSFHVEGLVAIILFYLLIFLVGIWAAWKTKNSGNPEERSEAIIVGGRDIGLLVGGFTMTATWVGGGYINGTAEAVYGPGCGLAWAQAPIGYSLSLILGGLFFAKPMRSKGYVTMLDPFQQIYGKRMGGLLFIPALMGEMFWAAAIFSALGATISVIIDVDVNISVIVSALIAILYTLVGGLYSVAYTDVVQLFCIFIGLWISVPFALSHPAVTDIGFTAVHAKYQSPWLGTIESVEVYTWLDNFLLLMLGGIPWQAYFQRVLSSSSATYAQVLSFLAAFGCLVMALPAICIGAIGASTDWNQTAYGYPDPKTKEEADMILPIVLQYLCPVYISFFGLGAVSAAVMSSADSSILSASSMFARNIYQLSFRQNASDKEIVWVMRITVLVFGASATAMALLTKTVYGLWYLSSDLVYIIIFPQLLCVLFIKGTNTYGAVAGYIFGLFLRITGGEPYLYLQPLIFYPGYYSDKNGIYNQRFPFKTLSMVTSFFTNICVSYLAKYLFESGTLPPKLDVFDAVVARHSEENMDKTILVRNENIKLNELAPVKPRQSLTLSSTFTNKEALLDVDSSPEGSGTEDNLQ 6154CE6622772A41 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 aplp1_human Amyloid beta precursor like protein 1 APLP1 Amyloid beta (A4) precursor-like protein 1 Amyloid-like protein 1 protein protein human Homo sapiens Human MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP DIP protein P51693 original sequence version: 82 DIP protein P51693-1 original sequence version: 124 B95F0F4D1C5CBAC7 a4-slc5a7-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Immunoprecipitation and Western blotting (IP-WB) of HEK293 cells transfected with full-length APP (APP-FL) and CHT. Fig. 4 a4-slc5a7-2 1 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey c-terminal fragment sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association Immunoprecipitation and Western blotting (IP-WB) of HEK293 cells transfected with full-length APP (APP-FL) and CHT. Fig. 4A and B Author described the APP construct as a C-terminal fragment. There are 3 naturally occuring C-terminal fragment, the positions in the sequence have been annotated to the longest of these. Because of the fuzziness of the sequence positions I have annotated to the full-length protein, not dealt with it as a chain. aplp1-slc5a7-1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey c-terminal fragment sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association IP-WB analysis of HEK293 cells transfected with CHT and C-terminal fragment (CTF) of APP (APP-CTF), APLP1 (APLP1-CTF), Fig. 4B Author describes the APLP1 construct as a C-terminal fragment. This normally refers to the C30 cleaved product, so this is sequence length given for the protein, but this is not made clear in the article. because of the fuzziness of the coordinates, I've dealt with this as a fragment of the full length protein rather than a chain. aplp2-slc5a7-2 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey c-terminal fragment sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association IP-WB analysis of HEK293 cells transfected with CHT and C-terminal fragment (CTF) of APP (APP-CTF), APLP1 (APLP1-CTF), Fig. 4B Author describes the APLP2 construct as a C-terminal fragment. The coordinates of the cytoplasmic domain as given in UniProtKB have been used for this, as exact coordinates are not given in the article.