IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Homo- and heterodimerization of APP family members promotes intercellular adhesion. Homo- and heterodimerization of APP family members promotes intercellular adhesion. EMBO J. (0261-4189) 2005 imex curation Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de 16-Dec-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de Accepted 2008-Not a month!-10 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Homo- and heterodimerization of APP family members promotes intercellular adhesion. Homo- and heterodimerization of APP family members promotes intercellular adhesion. EMBO J. (0261-4189) 2005 imex curation Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de 16-Dec-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de Accepted 2008-Not a month!-10 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Homo- and heterodimerization of APP family members promotes intercellular adhesion. Homo- and heterodimerization of APP family members promotes intercellular adhesion. EMBO J. (0261-4189) 2005 imex curation Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de 16-Dec-2008: Contacted by JYOTI. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de Accepted 2008-Not a month!-10 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation Homo- and heterodimerization of APP family members promotes intercellular adhesion. Homo- and heterodimerization of APP family members promotes intercellular adhesion. EMBO J. (0261-4189) 2005 imex curation Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. Alzheimers - Interactions investigated in the context of Alzheimers disease Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de 16-Dec-2008: Contacted by JYOTI. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Soba P., Eggert S., Wagner K., Zentgraf H., Siehl K., Kreger S., Lower A., Langer A., Merdes G., Paro R., Masters CL., Muller U., Kins S., Beyreuther K. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional methods available in supplementary information. myc tagged and HA tagged APP, APLP1 or APLP2 transiently expressed in Cos-7 cells. Homo- and heterodimerization detected by western blot with anti HA and anti-myc antibodies. p.soba@zmbh.uni-heidelberg.de Accepted 2008-Not a month!-10 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2005 Only protein-protein interactions imex curation aplp1_mouse Amyloid beta precursor like protein 1 Amyloid-like protein 1 Amyloid beta (A4) precursor-like protein 1 Aplp1 protein protein mouse Mus musculus Mouse MGPTSPAARGQGRRWRPPPLPLLLPLSLLLLRAQLAVGNLAVGSPSAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLLDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWCGGTRSGRCAHPHHEVVPFHCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPATPNPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEEERAPPPSSHTPVMVSRVTPTPRPTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHLAQELRPQIQELLLAEHLGPSELDASVPGSSSEDKGSLQPPESKDDPPVTLPKGSTDQESSSSGREKLTPLEQYEQKVNASAPRGFPFHSSDIQRDELAPSGTGVSREALSGLLIMGAGGGSLIVLSLLLLRKKKPYGTISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP D72A3065D8D95F68 aplp2_human Amyloid beta precursor like protein 2 APPH APPL2 APLP2 Amyloid protein homolog CDEI box-binding protein Amyloid beta (A4) precursor-like protein 2 Amyloid-like protein 2 Sperm membrane protein YWK-II protein protein human Homo sapiens Human MAATGTAAAAATGRLLLLLLVGLTAPALALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCFETKEEVLQYCQEMYPELQITNVMEANQRVSIDNWCRRDKKQCKSRFVTPFKCLVGEFVSDVLLVPEKCQFFHKERMEVCENHQHWHTVVKEACLTQGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKIIGSVSKEEEEEDEEEEEEEDEEEDYDVYKSEFPTEADLEDFTEAAVDEDDEDEEEGEEVVEDRDYYYDTFKGDDYNEENPTEPGSDGTMSDKEITHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKAMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRMALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTASISETPVDVRVSSEESEEIPPFHPFHPFPALPENEDTQPELYHPMKKGSGVGEQDGGLIGAEEKVINSKNKVDENMVIDETLDVKEMIFNAERVGGLEEERESVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI DIP protein Q06481 original sequence version: 94 CA3A7D6DDB8A28D0 a4_mouse Amyloid-beta precursor protein App Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 988D89E089092A3E aplp1_human Amyloid beta precursor like protein 1 APLP1 Amyloid beta (A4) precursor-like protein 1 Amyloid-like protein 1 protein protein human Homo sapiens Human MGPASPAARGLSRRPGQPPLPLLLPLLLLLLRAQPAIGSLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTLEEQQLRELQRHGYENPTYRFLEERP DIP protein P51693 original sequence version: 82 DIP protein P51693-1 original sequence version: 124 B95F0F4D1C5CBAC7 aplp2_mouse Amyloid beta precursor like protein 2 APPL2 Amyloid beta (A4) precursor-like protein 2 Amyloid protein homolog Amyloid-like protein 2 Sperm membrane protein YWK-II CDEI box-binding protein Aplp2 protein protein mouse Mus musculus Mouse MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKRQCKSHIVIPFKCLVGEFVSDVLLVPDNCQFFHQERMEVCEKHQRWHTLVKEACLTEGLTLYSYGMLLPCGVDQFHGTEYVCCPQTKTVDSDSTMSKEEEEEEEDEEDEEEDYDLDKSEFPTEADLEDFTEAAADEEEEDEEEGEEVVEDRDYYYDPFKGDDYNEENPTEPSSEGTISDKEIVHDVKVPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRNRMDRVKKEWEEAELQAKNLPKTERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPLHPFPSLSENEDTQPELYHPMKKGSGMAEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPESVGPLREDFSLSSNALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI BBB8C3E25A6CC503 p05067-4 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 695 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067-4 original sequence version: 183 DIP protein P05067-4 original sequence version: 198 DIP protein P05067-4 original sequence version: 224 A major isoform. 4F6EA0139F969D56 app-app-1 2 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 10 unspecified role unspecified role prey prey app e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-aplp1 2 8 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp2-aplp2 2 6 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range e1 domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-app 2 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 app-aplp2 2 6 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range e1 region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 10 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp2-aplp1 2 8 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 6 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range e1 region required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 app-app-2 1 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 3 aplp1-aplp1-1 1 8 unspecified role unspecified role unspecified role unspecified role region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction Fig 3 aplp2-aplp2-1 1 6 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig 3 aplp1-app-1 3 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey direct interaction direct interaction Fig 6 C-terminal antibody 57 used to immunoprecipitate APLP1. At least 3 distinct forms of APP with molecular mass of 100, 108, and 120 KDa were detected likely representing different post-translationally modified APP 695 species. Only the 108 and 120 forms were coimmunoprcipated with APLP1. app-aplp2-1 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait direct interaction direct interaction Fig 6 APP immunoprecipated with antibody 22734. APLP2 only detected in coimmunoprecipates from WT and APLP1-/- brains. aplp1-aplp2 3 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait direct interaction direct interaction Fig 6 APLP1 immunoprecipated with antibody 57. APLP2 only detected in coimmunoprecipates from WT and APP-/- brains. Only the mature form (120kDa) of APLP2 coimmunoprecipitated with APLP1. aplp1-app-2 4 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig 7C C-terminal antibody 57 used to immunoprecipitate APLP1. 108, and 120 KDa forms of APP specifically coimmunoprecipated. aplp1-app-4 2 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 aplp2-aplp1-1 2 6 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 app-aplp2-2 2 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 6 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 app-app-3 2 10 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait app e1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Fig 1, Fig 2 and supplementary Fig 1 aplp1-aplp1-2 2 8 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 8 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1 aplp2-aplp2-2 2 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 6 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association Supplementary methods describe in detail the deletion constructs and APP and APLP1 as having N-terminal myc/HA tags and APLP2 as having C-terminal myc/HA tags. Supplementary Fig 1