IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). J. Biol. Chem. (0021-9258) 2004 imex curation Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Library - MATCHMAKER cDNA library (in the pGADT7 vector) derived from HeLa cells (Clontech) Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Library - MATCHMAKER cDNA library (in the pGADT7 vector) derived from HeLa cells (Clontech) J. Biol. Chem. (0021-9258) 2004 IMEx Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). J. Biol. Chem. (0021-9258) 2004 imex curation Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Library - MATCHMAKER cDNA library (in the pGADT7 vector) derived from HeLa cells (Clontech) Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2004 IMEx Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). Identification of snapin and three novel proteins (BLOS1, BLOS2, and BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome-related organelles complex-1 (BLOC-1). J. Biol. Chem. (0021-9258) 2004 imex curation Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Library - MATCHMAKER cDNA library (in the pGADT7 vector) derived from HeLa cells (Clontech) Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Starcevic M., Dell'Angelica EC. Synapse - Interactions of proteins with an established role in the presynapse. Cells were radiolabelled and participant identification was further confirmed by fluorography. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Library - MATCHMAKER cDNA library (in the pGADT7 vector) derived from HeLa cells (Clontech) J. Biol. Chem. (0021-9258) 2004 IMEx bl1s6_human Biogenesis of lysosome-related organelles complex 1 subunit 6 BLOC1S6 PA PLDN Pallid protein homolog Pallidin Syntaxin 13-interacting protein protein protein human Homo sapiens Human MSVPGPSSPDGALTRPPYCLEAGEPTPGLSDTSPDEGLIEDLTIEDKAVEQLAEGLLSHYLPDLQRSKQALQELTQNQVVLLDTLEQEISKFKECHSMLDINALFAEAKHYHAKLVNIRKEMLMLHEKTSKLKKRALKLQQKRQKEELEREQQREKEFEREKQLTARPAKRM 37902FCFD4802294 bl1s5_human Biogenesis of lysosome-related organelles complex 1 subunit 5 BLOC1S5 MUTED Protein Muted homolog protein protein human Homo sapiens Human MSGGGTETPVGCEAAPGGGSKKRDSLGTAGSAHLIIKDLGEIHSRLLDHRPVIQGETRYFVKEFEEKRGLREMRVLENLKNMIHETNEHTLPKCRDTMRDSLSQVLQRLQAANDSVCRLQQREQERKKIHSDHLVASEKQHMLQWDNFMKEQPNKRAEVDEEHRKAMERLKEQYAEMEKDLAKFSTF 790D4DE8E97D83D1 bl1s4_human Biogenesis of lysosome-related organelles complex 1 subunit 4 BLOC1S4 CNO Protein cappuccino homolog protein protein human Homo sapiens Human MEGSFSDGGALPEGLAEEAEPQGAAWSGDSGTVSQSHSSASGPWEDEGAEDGAPGRDLPLLRRAAAGYAACLLPGAGARPEVEALDASLEDLLTRVDEFVGMLDMLRGDSSHVVSEGVPRIHAKAAEMRRIYSRIDRLEAFVRMVGGRVARMEEQVTKAEAELGTFPRAFKKLLHTMNVPSLFSKSAPSRPQQAGYEAPVLFRTEDYFPCCSERPQL 46CFA7105DAF1071 snapn_human SNARE-associated protein Snapin SNAPIN SNAP25BP SNAPAP Synaptosomal-associated protein 25-binding protein BLOC1S7 Biogenesis of lysosome-related organelles complex 1 subunit 7 protein protein human Homo sapiens Human MAGAGSAAVSGAGTPVAGPTGRDLFAEGLLEFLRPAVQQLDSHVHAVRESQVELREQIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRRAMLDSGIYPPGSPGK 3EA402AC53C81FFF bl1s3_human Biogenesis of lysosome-related organelles complex 1 subunit 3 BLOC1S3 BLOS3 protein protein human Homo sapiens Human MASQGRRRRPLRRPETVVPGEATETDSERSASSSEEEELYLGPSGPTRGRPTGLRVAGEAAETDSEPEPEPEPTAAPRDLPPLVVQRESAEEAWGTEEAPAPAPARSLLQLRLAESQARLDHDVAAAVSGVYRRAGRDVAALASRLAAAQAAGLAAAHSVRLARGDLCALAERLDIVAGCRLLPDIRGVPGTEPEKDPGPRA EB072B856AB6B4CF bl1s1_human Biogenesis of lysosome-related organelles complex 1 subunit 1 BLOC1S1 BLOS1 RT14 GCN5L1 GCN5-like protein 1 Protein RT14 protein protein human Homo sapiens Human MAPGSRGERSSFRSRRGPGVPSPQPDVTMLSRLLKEHQAKQNERKELQEKRRREAITAATCLTEALVDHLNVGVAQAYMNQRKLDHEVKTLQVQAAQFAKQTGQWIGMVENFNQALKEIGDVENWARSIELDMRTIATALEYVYKGQLQSAPS B7D1FE64F242B25A dtbp1_human Dysbindin My031 DTNBP1 Dystrobrevin-binding protein 1 Hermansky-Pudlak syndrome 7 protein Dysbindin-1 BLOC1S8 Biogenesis of lysosome-related organelles complex 1 subunit 8 protein protein human Homo sapiens Human MLETLRERLLSVQQDFTSGLKTLSDKSREAKVKSKPRTVPFLPKYSAGLELLSRYEDTWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTHLEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQMDLMDISDQEALDVFLNSGGEENTVLSPALGPESSTCQNEITLQVPNPSELRAKPPSSSSTCTDSATRDISEGGESPVVQSDEEEVQVDTALATSHTDREATPDGGEDSDS 0504C86E12B66C08 bl1s2_human Biogenesis of lysosome-related organelles complex 1 subunit 2 BLOC1S2 BLOS2 CEAP Centrosome-associated protein protein protein human Homo sapiens Human MAAAAEGVLATRSDEPARDDAAVETAEEAKEPAEADITELCRDMFSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAGLQPYLDQINVIEEQVAALEQAAYKLDAYSKKLEAKYKKLEKR 023DDBAC66E1912B pldn-dtnbp1-1 1 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association pldn-bloc1s1-1 1 4 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association pldn-pldn-1 2 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig. 6B pldn-dtnbp1-2 2 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 6A pldn-bloc1s1-2 2 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait physical association physical association Fig. 2A, 6A pldn-cno-1 2 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 6A muted-dtnbp1-1 2 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 6A muted-bloc1s2-1 2 5 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig. 6A cno-pldn-1 2 6 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig. 6A cno-snapap-1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 6A cno-bloc1s2-1 2 6 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig. 6A snapap-bloc1s2-1 2 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig. 6A snapap-dtnbp1-1 2 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 6A bloc1s1-pldn-1 2 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2B, 6A bloc1s1-snapap-1 2 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 6A bloc1s1-bloc1s2-1 2 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 6A bloc1s2-cno-1 2 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 6A bloc1s2-snapap-1 2 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 6A bloc1s3-bloc1s2-1 2 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig. 6A pldn-bloc1s1-3 3 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Fig. 4 bloc1s2-bloc1s1-1 3 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4 dtnbp1-bloc1s1-1 3 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait association association Fig. 4 bloc1s3-bloc1s1-1 3 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig 4