IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Synapse - Interactions of proteins with an established role in the presynapse. Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Synapse - Interactions of proteins with an established role in the presynapse. Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Synapse - Interactions of proteins with an established role in the presynapse. Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. (0006-291X) 2003 imex curation Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Magklara A., Mellati AA., Wasney GA., Little SP., Sotiropoulou G., Becker GW., Diamandis EP. Synapse - Interactions of proteins with an established role in the presynapse. Klk6 was expressed in 293 cells stably transfected with plasmid encoding Klk6. Cells were grown for 3 weeks and cell supernatents were concentrated and stored at 4 degrees for 1 week to allow autoactivation. Enzyme was then purified by reverse phase HPLC. Klk6 was mixed with proteins or fluorescent complexes in reaction buffer. Reaction mixes were incubated at 37 degrees for up to 8 hours. Aliquots taken at different time points were analysed by SDS page and coomassie staining. ediamandis@mtsinai.on.ca Biochem. Biophys. Res. Commun. (0006-291X) 2003 Only protein-protein interactions imex curation co1a2_bovin Collagen alpha-2(I) chain COL1A2 Alpha-2 type I collagen protein protein bovin Bos taurus (Bovine) Bovine MLSFVDTRTLLLLAVTSCLATCQSLQEATARKGPSGDRGPRGERGPPGPPGRDGDDGIPGPPGPPGPPGPPGLGGNFAAQFDAKGGGPGPMGLMGPRGPPGASGAPGPQGFQGPPGEPGEPGQTGPAGARGPPGPPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGENGTPGQTGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPGPAGPAGPRGEVGLPGLSGPVGPPGNPGANGLPGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATGARGLVGEPGPAGSKGESGNKGEPGAVGQPGPPGPSGEEGKRGSTGEIGPAGPPGPPGLRGNPGSRGLPGADGRAGVMGPAGSRGATGPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPSGDPGKAGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGLPGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGTKGPKGENGPVGPTGPVGAAGPSGPNGPPGPAGSRGDGGPPGATGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRSGETGASGPPGFVGEKGPSGEPGTAGPPGTPGPQGLLGAPGFLGLPGSRGERGLPGVAGSVGEPGPLGIAGPPGARGPPGNVGNPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNAGPVGAAGAPGPQGPVGPVGKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGPAGPRGPAGPSGPAGKDGRIGQPGAVGPAGIRGSQGSQGPAGPPGPPGPPGPPGPSGGGYEFGFDGDFYRADQPRSPTSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTGETCIRAQPEDIPVKNWYRNSKAKKHVWVGETINGGTQFEYNVEGVTTKEMATQLAFMRLLANHASQNITYHCKNSIAYMDEETGNLKKAVILQGSNDVELVAEGNSRFTYTVLVDGCSKKTNEWQKTIIEYKTNKPSRLPILDIAPLDIGGADQEIRLNIGPVCFK 5593F4D6B9ED119A fiba_human Fibrinogen alpha chain FGA protein protein human Homo sapiens Human MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRDCDDVLQTHPSGTQSGIFNIKLPGSSKIFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDEGEGEFWLGNDYLHLLTQRGSVLRVELEDWAGNEAYAEYHFRVGSEAEGYALQVSSYEGTAGDALIEGSVEEGAEYTSHNNMQFSTFDRDADQWEENCAEVYGGGWWYNNCQAANLNGIYYPGGSYDPRNNSPYEIENGVVWVSFRGADYSLRAVRMKIRPLVTQ DIP protein P02671 original sequence version: 118 DIP protein P02671-1 original sequence version: 160 DIP protein P02671 original sequence version: 157 EA73A81204D8AEC4 aspproargamc Asp-Pro-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt co4a3_human Collagen alpha-3(IV) chain COL4A3 Goodpasture antigen protein protein human Homo sapiens Human MSARTAPRPQVLLLPLLLVLLAAAPAASKGCVCKDKGQCFCDGAKGEKGEKGFPGPPGSPGQKGFTGPEGLPGPQGPKGFPGLPGLTGSKGVRGISGLPGFSGSPGLPGTPGNTGPYGLVGVPGCSGSKGEQGFPGLPGTLGYPGIPGAAGLKGQKGAPAKEEDIELDAKGDPGLPGAPGPQGLPGPPGFPGPVGPPGPPGFFGFPGAMGPRGPKGHMGERVIGHKGERGVKGLTGPPGPPGTVIVTLTGPDNRTDLKGEKGDKGAMGEPGPPGPSGLPGESYGSEKGAPGDPGLQGKPGKDGVPGFPGSEGVKGNRGFPGLMGEDGIKGQKGDIGPPGFRGPTEYYDTYQEKGDEGTPGPPGPRGARGPQGPSGPPGVPGSPGSSRPGLRGAPGWPGLKGSKGERGRPGKDAMGTPGSPGCAGSPGLPGSPGPPGPPGDIVFRKGPPGDHGLPGYLGSPGIPGVDGPKGEPGLLCTQCPYIPGPPGLPGLPGLHGVKGIPGRQGAAGLKGSPGSPGNTGLPGFPGFPGAQGDPGLKGEKGETLQPEGQVGVPGDPGLRGQPGRKGLDGIPGTPGVKGLPGPKGELALSGEKGDQGPPGDPGSPGSPGPAGPAGPPGYGPQGEPGLQGTQGVPGAPGPPGEAGPRGELSVSTPVPGPPGPPGPPGHPGPQGPPGIPGSLGKCGDPGLPGPDGEPGIPGIGFPGPPGPKGDQGFPGTKGSLGCPGKMGEPGLPGKPGLPGAKGEPAVAMPGGPGTPGFPGERGNSGEHGEIGLPGLPGLPGTPGNEGLDGPRGDPGQPGPPGEQGPPGRCIEGPRGAQGLPGLNGLKGQQGRRGKTGPKGDPGIPGLDRSGFPGETGSPGIPGHQGEMGPLGQRGYPGNPGILGPPGEDGVIGMMGFPGAIGPPGPPGNPGTPGQRGSPGIPGVKGQRGTPGAKGEQGDKGNPGPSEISHVIGDKGEPGLKGFAGNPGEKGNRGVPGMPGLKGLKGLPGPAGPPGPRGDLGSTGNPGEPGLRGIPGSMGNMGMPGSKGKRGTLGFPGRAGRPGLPGIHGLQGDKGEPGYSEGTRPGPPGPTGDPGLPGDMGKKGEMGQPGPPGHLGPAGPEGAPGSPGSPGLPGKPGPHGDLGFKGIKGLLGPPGIRGPPGLPGFPGSPGPMGIRGDQGRDGIPGPAGEKGETGLLRAPPGPRGNPGAQGAKGDRGAPGFPGLPGRKGAMGDAGPRGPTGIEGFPGPPGLPGAIIPGQTGNRGPPGSRGSPGAPGPPGPPGSHVIGIKGDKGSMGHPGPKGPPGTAGDMGPPGRLGAPGTPGLPGPRGDPGFQGFPGVKGEKGNPGFLGSIGPPGPIGPKGPPGVRGDPGTLKIISLPGSPGPPGTPGEPGMQGEPGPPGPPGNLGPCGPRGKPGKDGKPGTPGPAGEKGNKGSKGEPGPAGSDGLPGLKGKRGDSGSPATWTTRGFVFTRHSQTTAIPSCPEGTVPLYSGFSFLFVQGNQRAHGQDLGTLGSCLQRFTTMPFLFCNVNDVCNFASRNDYSYWLSTPALMPMNMAPITGRALEPYISRCTVCEGPAIAIAVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRASPFLECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMKKRH AA65D50903D82B99 glnglyargamc Gln-Gly-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt propheargamc Pro-Phe-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt casa1_human Alpha-S1-casein CSN1S1 CASA CSN1 protein protein human Homo sapiens Human MRLLILTCLVAVALARPKLPLRYPERLQNPSESSEPIPLESREEYMNGMNRQRNILREKQTDEIKDTRNESTQNCVVAEPEKMESSISSSSEEMSLSKCAEQFCRLNEYNQLQLQAAHAQEQIRRMNENSHVQVPFQQLNQLAAYPYAVWYYPQIMQYVPFPPFSDISNPTAHENYEKNNVMLQW A7717899CDC7D563 co1a1_bovin Collagen alpha-1(I) chain COL1A1 Alpha-1 type I collagen protein protein bovin Bos taurus (Bovine) Bovine MFSFVDLRLLLLLAATALLTHGQEEGQEEGQEEDIPPVTCVQNGLRYHDRDVWKPVPCQICVCDNGNVLCDDVICDELKDCPNAKVPTDECCPVCPEGQESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGNDGATGAAGPPGPTGPAGPPGFPGAVGAKGEGGPQGPRGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGPPGPKGNSGEPGAPGSKGDTGAKGEPGPTGIQGPPGPAGEEGKRGARGEPGPAGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDKGEAGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPAGRPGEVGPPGPPGPAGEKGAPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPIGPVGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDAGPAGPPGPPGPPGPPGPPSGGYDLSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKEKRHVWYGESMTGGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTYDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPACFL 8A6E17F276C4C6FA co4a4_human Collagen alpha-4(IV) chain COL4A4 protein protein human Homo sapiens Human MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS C55711CDF14A57DB pheserargamc Phe-Ser-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt co4a1_human Collagen alpha-1(IV) chain COL4A1 protein protein human Homo sapiens Human MGPRLSVWLLLLPAALLLHEEHSRAAAKGGCAGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFAGNPGPPGLPGMKGDPGEILGHVPGMLLKGERGFPGIPGTPGPPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGLSFQGPKGDKGDQGVSGPPGVPGQAQVQEKGDFATKGEKGQKGEPGFQGMPGVGEKGEPGKPGPRGKPGKDGDKGEKGSPGFPGEPGYPGLIGRQGPQGEKGEAGPPGPPGIVIGTGPLGEKGERGYPGTPGPRGEPGPKGFPGLPGQPGPPGLPVPGQAGAPGFPGERGEKGDRGFPGTSLPGPSGRDGLPGPPGSPGPPGQPGYTNGIVECQPGPPGDQGPPGIPGQPGFIGEIGEKGQKGESCLICDIDGYRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGVAGVPGPQGTPGLIGQPGAKGEPGEFYFDLRLKGDKGDPGFPGQPGMPGRAGSPGRDGHPGLPGPKGSPGSVGLKGERGPPGGVGFPGSRGDTGPPGPPGYGPAGPIGDKGQAGFPGGPGSPGLPGPKGEPGKIVPLPGPPGAEGLPGSPGFPGPQGDRGFPGTPGRPGLPGEKGAVGQPGIGFPGPPGPKGVDGLPGDMGPPGTPGRPGFNGLPGNPGVQGQKGEPGVGLPGLKGLPGLPGIPGTPGEKGSIGVPGVPGEHGAIGPPGLQGIRGEPGPPGLPGSVGSPGVPGIGPPGARGPPGGQGPPGLSGPPGIKGEKGFPGFPGLDMPGPKGDKGAQGLPGITGQSGLPGLPGQQGAPGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPGLPGEKGDHGFPGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKDGQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKGQAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGSPGLPGEKGDKGLPGLDGIPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEPGLPGRGFPGFPGAKGDKGSKGEVGFPGLAGSPGIPGSKGEQGFMGPPGPQGQPGLPGSPGHATEGPKGDRGPQGQPGLPGLPGPMGPPGLPGIDGVKGDKGNPGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGPKGLPGLQGIKGDQGDQGVPGAKGLPGPPGPPGPYDIIKGEPGLPGPEGPPGLKGLQGLPGPKGQQGVTGLVGIPGPPGIPGFDGAPGQKGEMGPAGPTGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTIDDPQCPSGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGENIRPFISRCAVCEAPAMVMAVHSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT 3BEBA6DFFB9B8A84 klk6_human Kallikrein-6 PRSS9 KLK6 PRSS18 Protease M Neurosin Zyme SP59 Serine protease 9 Serine protease 18 protein protein human Homo sapiens Human MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAK AEA03F9145D87AAB valproargamc Val-Pro-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt glyglyargamc Gly-Gly-Arg-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt co4a2_human Collagen alpha-2(IV) chain COL4A2 protein protein human Homo sapiens Human MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLAGVKKFDVPCGGRDCSGGCQCYPEKGGRGQPGPVGPQGYNGPPGLQGFPGLQGRKGDKGERGAPGVTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGTQGDSGPQGPPGSEGFTGPPGPQGPKGQKGEPYALPKEERDRYRGEPGEPGLVGFQGPPGRPGHVGQMGPVGAPGRPGPPGPPGPKGQQGNRGLGFYGVKGEKGDVGQPGPNGIPSDTLHPIIAPTGVTFHPDQYKGEKGSEGEPGIRGISLKGEEGIMGFPGLRGYPGLSGEKGSPGQKGSRGLDGYQGPDGPRGPKGEAGDPGPPGLPAYSPHPSLAKGARGDPGFPGAQGEPGSQGEPGDPGLPGPPGLSIGDGDQRRGLPGEMGPKGFIGDPGIPALYGGPPGPDGKRGPPGPPGLPGPPGPDGFLFGLKGAKGRAGFPGLPGSPGARGPKGWKGDAGECRCTEGDEAIKGLPGLPGPKGFAGINGEPGRKGDRGDPGQHGLPGFPGLKGVPGNIGAPGPKGAKGDSRTITTKGERGQPGVPGVPGMKGDDGSPGRDGLDGFPGLPGPPGDGIKGPPGDPGYPGIPGTKGTPGEMGPPGLGLPGLKGQRGFPGDAGLPGPPGFLGPPGPAGTPGQIDCDTDVKRAVGGDRQEAIQPGCIGGPKGLPGLPGPPGPTGAKGLRGIPGFAGADGGPGPRGLPGDAGREGFPGPPGFIGPRGSKGAVGLPGPDGSPGPIGLPGPDGPPGERGLPGEVLGAQPGPRGDAGVPGQPGLKGLPGDRGPPGFRGSQGMPGMPGLKGQPGLPGPSGQPGLYGPPGLHGFPGAPGQEGPLGLPGIPGREGLPGDRGDPGDTGAPGPVGMKGLSGDRGDAGFTGEQGHPGSPGFKGIDGMPGTPGLKGDRGSPGMDGFQGMPGLKGRPGFPGSKGEAGFFGIPGLKGLAGEPGFKGSRGDPGPPGPPPVILPGMKDIKGEKGDEGPMGLKGYLGAKGIQGMPGIPGLSGIPGLPGRPGHIKGVKGDIGVPGIPGLPGFPGVAGPPGITGFPGFIGSRGDKGAPGRAGLYGEIGATGDFGDIGDTINLPGRPGLKGERGTTGIPGLKGFFGEKGTEGDIGFPGITGVTGVQGPPGLKGQTGFPGLTGPPGSQGELGRIGLPGGKGDDGWPGAPGLPGFPGLRGIRGLHGLPGTKGFPGSPGSDIHGDPGFPGPPGERGDPGEANTLPGPVGVPGQKGDQGAPGERGPPGSPGLQGFPGITPPSNISGAPGDKGAPGIFGLKGYRGPPGPPGSAALPGSKGDTGNPGAPGTPGTKGWAGDSGPQGRPGVFGLPGEKGPRGEQGFMGNTGPTGAVGDRGPKGPKGDPGFPGAPGTVGAPGIAGIPQKIAVQPGTVGPQGRRGPPGAPGEMGPQGPPGEPGFRGAPGKAGPQGRGGVSAVPGFRGDEGPIGHQGPIGQEGAPGRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL E0DABEEAB349D8AF fibb_human Fibrinogen beta chain FGB protein protein human Homo sapiens Human MKRMVSWSFHKLKTMKHLLLLLLCVFLVKSQGVNDNEEGFFSARGHRPLDKKREEAPSLRPAPPPISGGGYRARPAKAAATQKKVERKAPDAGGCLHADPDLGVLCPTGCQLQEALLQQERPIRNSVDELNNNVEAVSQTSSSSFQYMYLLKDLWQKRQKQVKDNENVVNEYSSELEKHQLYIDETVNSNIPTNLRVLRSILENLRSKIQKLESDVSAQMEYCRTPCTVSCNIPVVSGKECEEIIRKGGETSEMYLIQPDSSVKPYRVYCDMNTENGGWTVIQNRQDGSVDFGRKWDPYKQGFGNVATNTDGKNYCGLPGEYWLGNDKISQLTRMGPTELLIEMEDWKGDKVKAHYGGFTVQNEANKYQISVNKYRGTAGNALMDGASQLMGENRTMTIHNGMFFSTYDRDNDGWLTSDPRKQCSKEDGGGWWYNRCHAANPNGRYYWGGQYTWDMAKHGTDDGVVWMNWKGSWYSMRKMSMKIRPFFPQQ DIP protein P02675 original sequence version: 111 DIP protein P02675 original sequence version: 153 DIP protein P02675 original sequence version: 149 DIP protein P02675 original sequence version: 147 B92FFB9976AB53C5 valleulysamc Val-Leu-Lys-7-amino-4-methylcoumarin synthetic peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro An amino acid sequence which is not represented in UniProt co4a5_human Collagen alpha-5(IV) chain COL4A5 protein protein human Homo sapiens Human MKLRGVSLAAGLFLLALSLWGQPAEAAACYGCSPGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNPGYPGPPGIQGLPGPTGIPGPIGPPGPPGLMGPPGPPGLPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDVEFQKGDQGLPGDRGPPGPPGIRGPPGPPGGEKGEKGEQGEPGKRGKPGKDGENGQPGIPGLPGDPGYPGEPGRDGEKGQKGDTGPPGPPGLVIPRPGTGITIGEKGNIGLPGLPGEKGERGFPGIQGPPGLPGPPGAAVMGPPGPPGFPGERGQKGDEGPPGISIPGPPGLDGQPGAPGLPGPPGPAGPHIPPSDEICEPGPPGPPGSPGDKGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGFPGQKGEKGQAGATGPKGLPGIPGAPGAPGFPGSKGEPGDILTFPGMKGDKGELGSPGAPGLPGLPGTPGQDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNIGPMGPPGFGPPGPVGEKGIQGVAGNPGQPGIPGPKGDPGQTITQPGKPGLPGNPGRDGDVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGLPGPPGPKGFPGIPGPPGAPGTPGRIGLEGPPGPPGFPGPKGEPGFALPGPPGPPGLPGFKGALGPKGDRGFPGPPGPPGRTGLDGLPGPKGDVGPNGQPGPMGPPGLPGIGVQGPPGPPGIPGPIGQPGLHGIPGEKGDPGPPGLDVPGPPGERGSPGIPGAPGPIGPPGSPGLPGKAGASGFPGTKGEMGMMGPPGPPGPLGIPGRSGVPGLKGDDGLQGQPGLPGPTGEKGSKGEPGLPGPPGPMDPNLLGSKGEKGEPGLPGIPGVSGPKGYQGLPGDPGQPGLSGQPGLPGPPGPKGNPGLPGQPGLIGPPGLKGTIGDMGFPGPQGVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKGEPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNPGLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLSGQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPPGRPGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGRPGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIKGDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT 4450A6762F12A626 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 co4a6_human Collagen alpha-6(IV) chain COL4A6 protein protein human Homo sapiens Human MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARGRPGPIGIQGPTGPQGFTGSTGLSGLKGERGFPGLLGPYGPKGDKGPMGVPGFLGINGIPGHPGQPGPRGPPGLDGCNGTQGAVGFPGPDGYPGLLGPPGLPGQKGSKGDPVLAPGSFKGMKGDPGLPGLDGITGPQGAPGFPGAVGPAGPPGLQGPPGPPGPLGPDGNMGLGFQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPKGFPGISGPPGFPGLGTTGEKGEKGEKGIPGLPGPRGPMGSEGVQGPPGQQGKKGTLGFPGLNGFQGIEGQKGDIGLPGPDVFIDIDGAVISGNPGDPGVPGLPGLKGDEGIQGLRGPSGVPGLPALSGVPGALGPQGFPGLKGDQGNPGRTTIGAAGLPGRDGLPGPPGPPGPPSPEFETETLHNKESGFPGLRGEQGPKGNLGLKGIKGDSGFCACDGGVPNTGPPGEPGPPGPWGLIGLPGLKGARGDRGSGGAQGPAGAPGLVGPLGPSGPKGKKGEPILSTIQGMPGDRGDSGSQGFRGVIGEPGKDGVPGLPGLPGLPGDGGQGFPGEKGLPGLPGEKGHPGPPGLPGNGLPGLPGPRGLPGDKGKDGLPGQQGLPGSKGITLPCIIPGSYGPSGFPGTPGFPGPKGSRGLPGTPGQPGSSGSKGEPGSPGLVHLPELPGFPGPRGEKGLPGFPGLPGKDGLPGMIGSPGLPGSKGATGDIFGAENGAPGEQGLQGLTGHKGFLGDSGLPGLKGVHGKPGLLGPKGERGSPGTPGQVGQPGTPGSSGPYGIKGKSGLPGAPGFPGISGHPGKKGTRGKKGPPGSIVKKGLPGLKGLPGNPGLVGLKGSPGSPGVAGLPALSGPKGEKGSVGFVGFPGIPGLPGIPGTRGLKGIPGSTGKMGPSGRAGTPGEKGDRGNPGPVGIPSPRRPMSNLWLKGDKGSQGSAGSNGFPGPRGDKGEAGRPGPPGLPGAPGLPGIIKGVSGKPGPPGFMGIRGLPGLKGSSGITGFPGMPGESGSQGIRGSPGLPGASGLPGLKGDNGQTVEISGSPGPKGQPGESGFKGTKGRDGLIGNIGFPGNKGEDGKVGVSGDVGLPGAPGFPGVAGMRGEPGLPGSSGHQGAIGPLGSPGLIGPKGFPGFPGLHGLNGLPGTKGTHGTPGPSITGVPGPAGLPGPKGEKGYPGIGIGAPGKPGLRGQKGDRGFPGLQGPAGLPGAPGISLPSLIAGQPGDPGRPGLDGERGRPGPAGPPGPPGPSSNQGDTGDPGFPGIPGPKGPKGDQGIPGFSGLPGELGLKGMRGEPGFMGTPGKVGPPGDPGFPGMKGKAGPRGSSGLQGDPGQTPTAEAVQVPPGPLGLPGIDGIPGLTGDPGAQGPVGLQGSKGLPGIPGKDGPSGLPGPPGALGDPGLPGLQGPPGFEGAPGQQGPFGMPGMPGQSMRVGYTLVKHSQSEQVPPCPIGMSQLWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPMMPVSQTQIPQYISRCSVCEAPSQAIAVHSQDITIPQCPLGWRSLWIGYSFLMHTAAGAEGGGQSLVSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERQQFGELPVSETLKAGQLHTRVSRCQVCMKSL 9313294D4CE63067 app-klk6 1 23 enzyme target enzyme target substrate substrate neutral component neutral component app_peptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cleavage site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage Fig 5 klk6-casa1 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 enzyme target enzyme target substrate substrate neutral component neutral component protein cleavage protein cleavage Fig 3A fgb-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 20 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 3B First, hK6 cleaves the Aa chain of the protein; this follows the pattern of plasmin digestion of fibrinogen, where the Aa carboxy-domains are rapidly cleaved, generating a 40-kDa fragment, which is subsequently degraded into smaller pieces [27]. At the same time, extensive digestion of chain Bb takes place, while the gamma chain appears intact. col1a1-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 12 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined bovin Bos taurus (Bovine) Bovine protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). col4a6-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 24 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. fga-klk6 2 16 enzyme enzyme unspecified role unspecified role klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 enzyme target enzyme target substrate substrate unspecified role unspecified role fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 3B First, hK6 cleaves the Aa chain of the protein; this follows the pattern of plasmin digestion of fibrinogen, where the Aa carboxy-domains are rapidly cleaved, generating a 40-kDa fragment, which is subsequently degraded into smaller pieces [27]. At the same time, extensive digestion of chain Bb takes place, while the gamma chain appears intact. col1a2-klk6 2 5 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined bovin Bos taurus (Bovine) Bovine 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). col4a1-klk6 2 15 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. col4a2-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 19 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. col4a3-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. col4a4-klk6 2 13 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. col4a5-klk6 2 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 22 enzyme target enzyme target substrate substrate neutral component neutral component fluorophore tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human protein cleavage protein cleavage Fig 4A Collagen type I is a better substrate for Klk6, being rapidly degraded by low enzyme concentrations (1uM). Collagen type IV degradation by Klk6 required longer incubation times and higher Klk6 concentrations (10uM). Collagen alpha chain composition is not specified in publication thus all 6 potential chains are included in the interaction curation. klk6-pheserargamc 3 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.41 kcat(min-1) = 38.3 kcat/Km (mM-1 min-1) = 93.4 An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. glyglyargamc-klk6 3 18 enzyme target enzyme target substrate substrate neutral component neutral component 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.455, kcat(min-1) = 6.5, kcat/Km (mM-1 min-1) = 14.3, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. aspproargamc-klk6 3 7 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.335, kcat(min-1) = 11.7, kcat/Km (mM-1 min-1) = 34.9, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. glnglyargamc-klk6 3 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.758, kcat(min-1) = 6.25, kcat/Km (mM-1 min-1) = 8.2, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. klk6-propheargamc 3 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 10 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.625, kcat(min-1) = 4.6, kcat/Km (mM-1 min-1) = 7.4, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. klk6-valproargamc 3 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 17 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage Table 1 Km(mM)= 0.271, kcat(min-1) = 22.8, kcat/Km (mM-1 min-1) = 84.4, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. klk6-valleulysamc 3 21 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 16 enzyme enzyme neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. cleavage cleavage reaction cleavage Table 1 Km(mM)= 1.72, kcat(min-1) = 0.22, kcat/Km (mM-1 min-1) = 0.13, An additional substrate, Glu-Lys-Lys-AMC was not hydrolysed by Klk6. Klk6 was also tested with a chymotrypsin-specific substrate, Ala-Ala-Pro-Phe-AMC, however no hydrolysis was detected, suggesting that human Klk6 posesses no such activity. klk6 4 16 unspecified role unspecified role neutral component neutral component klk6-propeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain arg-80_glu-81 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. protein cleavage protein cleavage Numbering for auto-cleavage site in figure legend and text differs from that used in the curation as it refers to a chemotrypsin based numbering of the amino acids, illustrated in reference 28. Fig 2