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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
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        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
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        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
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        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Prenylated Rab acceptor protein is a receptor for prenylated small GTPases.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="11335720" refType="primary-reference" refTypeAc="MI:0358"/>
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            <secondaryRef db="imex" dbAc="MI:0670" id="IM-26228" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Prenylated Rab acceptor protein is a receptor for prenylated small GTPases.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2001</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Figueroa C., Taylor J., Vojtek AB.</attribute>
            <attribute name="dataset" nameAc="MI:0875">Synapse - Interactions of proteins with an established role in the presynapse.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
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        </bibref>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="4932">
            <names>
              <shortLabel>yeasx</shortLabel>
              <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
              <alias type="synonym" typeAc="MI:1041">Baker's yeast</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>2 hybrid</shortLabel>
            <fullName>two hybrid</fullName>
            <alias type="go synonym" typeAc="MI:0303">2H</alias>
            <alias type="go synonym" typeAc="MI:0303">2h</alias>
            <alias type="go synonym" typeAc="MI:0303">Gal4 transcription regeneration</alias>
            <alias type="go synonym" typeAc="MI:0303">two-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">yeast two hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">2-hybrid</alias>
            <alias type="go synonym" typeAc="MI:0303">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">classical two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2H</alias>
            <alias type="synonym" typeAc="MI:1041">two-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">2-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Gal4 transcription regeneration</alias>
            <alias type="synonym" typeAc="MI:1041">2h</alias>
            <alias type="synonym" typeAc="MI:1041">yeast two hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">Y2H</alias>
            <alias type="synonym" typeAc="MI:1041">Y-2H</alias>
            <alias type="synonym" typeAc="MI:1041">2 hybrid</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0018" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Figueroa C., Taylor J., Vojtek AB.</attribute>
          <attribute name="dataset" nameAc="MI:0875">Synapse - Interactions of proteins with an established role in the presynapse.</attribute>
          <attribute name="negative">This experiment relates to a negative interaction.</attribute>
          <attribute name="accepted">Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01).</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2001</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>rap1a_human</shortLabel>
          <fullName>Ras-related protein Rap-1A</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">GTP-binding protein smg p21A</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Ras-related protein Krev-1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">C21KG</alias>
          <alias type="gene name synonym" typeAc="MI:0302">G-22K</alias>
          <alias type="gene name" typeAc="MI:0301">RAP1A</alias>
          <alias type="gene name synonym" typeAc="MI:0302">KREV1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P62834" version="SP_47" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P10113" version="SP_47" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000348786" version="SP_186" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001010935.1"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6798695" version="SP_186"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-9656223" version="SP_186"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_016857451.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_016857452.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_016857453.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-29106N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P62834 original sequence version: 37</attribute>
          <attribute name="remark-internal">DIP protein P62834 original sequence version: 93</attribute>
          <attribute name="crc64">42C39290C98E0A92</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>praf1_mouse</shortLabel>
          <fullName>Prenylated Rab acceptor protein 1</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">PRA1 family protein 1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Prenylin</alias>
          <alias type="gene name" typeAc="MI:0301">Rabac1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Pra</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Pra1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Praf1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9Z0S9" version="SP_41" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q3TDB4" version="SP_41" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-476965" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUSG00000003380" version="SP_142" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSMUST00000076961" version="SP_142" refType="transcript"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="SP_142"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_142"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0070064" version="SP_142"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR004895" version="SP_142"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_034391.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactorType>
        <organism ncbiTaxId="10090">
          <names>
            <shortLabel>mouse</shortLabel>
            <fullName>Mus musculus</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse</alias>
          </names>
        </organism>
        <sequence>MAAQKDQQKDAEGEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQMEPADGEELQMEPV</sequence>
        <attributeList>
          <attribute name="crc64">86C82C0502B5CB5B</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>rap1a-rabac1-2</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-492194" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>P62834:p.Gly12Val</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-492199" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation</shortLabel>
                    <fullName>mutation</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0118" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456558" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="12"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="12"/>
                    <resultingSequence>
                      <originalSequence>G</originalSequence>
                      <newSequence>V</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Wed May 02 19:33:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:36:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val'</attribute>
                </attributeList>
              </feature>
            </featureList>
          </participant>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <negative>true</negative>
        <attributeList>
          <attribute name="comment" nameAc="MI:0612">The mutation from gly-val makes the GTP-bound Ras mutant constitutively active. GTP required for activity.</attribute>
          <attribute name="remark-internal">Revisit when small molecules in place.</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>