IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. J. Biol. Chem. (0021-9258) 2001 imex curation Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2001 IMEx Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. J. Biol. Chem. (0021-9258) 2001 imex curation Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Only protein-protein interactions imex curation in vitro In vitro pull down pull down predetermined predetermined participant predetermined Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Human 293 cells have been transfected, and modified and unmodified GST-Ha-Ras was purified after Triton X-114 partitioning. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2001 IMEx Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. Prenylated Rab acceptor protein is a receptor for prenylated small GTPases. J. Biol. Chem. (0021-9258) 2001 imex curation Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Figueroa C., Taylor J., Vojtek AB. Synapse - Interactions of proteins with an established role in the presynapse. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Biol. Chem. (0021-9258) 2001 IMEx rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS DIP protein P01112 original sequence version: 91 DIP protein P01112 original sequence version: 106 DIP protein P01112 original sequence version: 144 DIP protein P01112 original sequence version: 118 DIP protein P01112 original sequence version: 143 DIP protein P01112 original sequence version: 152 DIP protein P01112-1 original sequence version: 159 DIP protein P01112 original sequence version: 170 DIP protein P01112-1 original sequence version: 185 DIP protein P01112 original sequence version: 192 DIP protein P01112-1 original sequence version: 196 DIP protein P01112-1 original sequence version: 202 EE6DC2D933E2856A rap1a_human Ras-related protein Rap-1A GTP-binding protein smg p21A Ras-related protein Krev-1 C21KG G-22K RAP1A KREV1 protein protein human Homo sapiens Human MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL DIP protein P62834 original sequence version: 37 DIP protein P62834 original sequence version: 93 42C39290C98E0A92 rhoa_human Transforming protein RhoA Rho cDNA clone 12 ARHA RHO12 RHOA ARH12 protein protein human Homo sapiens Human MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL DIP protein P61586 original sequence version: 86 DIP protein P61586 original sequence version: 123 DIP protein P61586 original sequence version: 57 DIP protein P61586 original sequence version: 88 DIP protein P61586 original sequence version: 87 DIP protein P61586 original sequence version: 85 DIP protein P61586 original sequence version: 125 DIP protein P61586 original sequence version: 132 C4DA2DC31FF858BC rras2_human Ras-related protein R-Ras2 Ras-like protein TC21 Teratocarcinoma oncogene RRAS2 TC21 protein protein human Homo sapiens Human MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF BA7D4759DC49446F praf1_mouse Prenylated Rab acceptor protein 1 PRA1 family protein 1 Prenylin Rabac1 Pra Pra1 Praf1 protein protein mouse Mus musculus Mouse MAAQKDQQKDAEGEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQMEPADGEELQMEPV 86C82C0502B5CB5B hras-rabac1-1 1 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P01112:p.Ser189delinsLysLysLysLysLysLys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S KKKKKK Wed May 02 15:21:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser189lys_lys_lys_lys_lys_lys' Tue Jun 30 17:08:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser189delinsLysLysLysLysLysLys' farnesylation-ser189 required farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm P01112:p.Gly12Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 18:55:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 20:24:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' physical association physical association Fig. 3 The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity. rras2-rabac1-1 1 7 unspecified role unspecified role bait bait P62070:p.Gly23Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 18:37:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly23val' Tue Jun 30 18:29:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly23Val' 8 unspecified role unspecified role prey prey physical association physical association Fig 2 The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity. hras-rabac1-2 3 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait P01112:p.Gly12Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 20:17:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 17:08:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:45:42 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'caax box' [EBI-491377]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 190-190, sequence length 189 [EBI-491377]187-190 region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity. Revisit when small molecules in place. rhoa-rabac1-2 3 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait P61586:p.Gln63Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:58:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln63leu' Tue Jun 30 16:58:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln63Leu' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association The mutation from Gln to Leu makes the GTP-bound Ras mutant constitutively active. GTP required for activity. Revisit when small molecules in place. rap1a-rabac1-1 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association hras-rabac1-3 2 8 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-492085]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 186-186, sequence length 185 [EBI-492085]20-186 region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined prenylation required modres protein modification ModRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5 The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity. Revisit when small molecules in place. hras-rabac1-4 1 8 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-492113]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 186-186, sequence length 185 [EBI-492113]20-186 4 unspecified role unspecified role bait bait P01112:p.Gly12Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 18:59:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 15:44:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' physical association physical association Fig. 2 The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity. rhoa-rabac1-3 1 8 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-492133]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 186-186, sequence length 185 [EBI-492133]20-186 6 unspecified role unspecified role bait bait P61586:p.Gln63Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:13:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln63leu' Tue Jun 30 16:36:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln63Leu' physical association physical association Fig 2 The mutation from Gln to Leu makes the GTP-bound Ras mutant constitutively active. GTP required for activity. Revisit when small molecules in place. rras2-rabac1-2 1 8 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-492147]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 186-186, sequence length 185 [EBI-492147]20-186 7 unspecified role unspecified role bait bait P62070:p.Gly23Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 17:21:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly23val' Tue Jun 30 18:38:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly23Val' physical association physical association Fig. 2 The mutation from Gly to Val makes the GTP-bound Ras mutant constitutively active. GTP required for activity.