UniProt UniProt http://www.uniprot.org SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. (0021-9258) 2000 Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu Accepted 2007-OCT-21 by DAVET. 2000 rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 davet SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. (0021-9258) 2000 Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library davet rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. PC12 cells have been treated with NGF to induce formation of neurites. lianli@med.unc.edu Accepted 2007-OCT-21 by DAVET. 2000 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 davet SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. (0021-9258) 2000 Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library davet in vitro In vitro pull down pull down antibody detection identification by antibody antibody detection Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu Accepted 2007-OCT-21 by DAVET. 2000 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 davet SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis. J. Biol. Chem. (0021-9258) 2000 Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chin LS., Nugent RD., Raynor MC., Vavalle JP., Li L. Synapse - Interactions of proteins with an established role in the presynapse. lianli@med.unc.edu Accepted 2007-OCT-21 by DAVET. 2000 12-Oct-2007: Contacted by JYOTI. rat hippocampal/cortical cDNA library J. Biol. Chem. (0021-9258) 2000 davet srcn1_rat SRC kinase signaling inhibitor 1 Srcin1 Snip p140Cap SNAP-25-interacting protein P140 p130Cas-associated protein protein protein rat Rattus norvegicus (Rat) Rat MQPWQCLRRFALAWWERTAEGRARSPREEVGPRDPGGRGEPDPERSSPPMLSADDAEYPREYRTLGGGGSGGSGGRRFSNVGLVHTSERRHTVIAAQSLEALSGLQKADADRKRDAFMDHLKSKYPQHALALRGQQDRMREQQPNYWSFKTRSSRHTQGAQPGLADQAAKLSYASAESLETMSEAELPLGFSRMNRFRQSLPLSRSASQTKLRSPGVLFLQFGEETRRVHITHEVSSLDTLHALIAHMFPQKLTMGMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPLYAAFPGSHLTNGDLRREMVYASRESSPTRRLNNLSPASHLASSSPPPGLPSGLPSGLPSGSPSRSRLSYAGGRPPSYAGSPVHHAAERLGGAPTSQGVSPSPSAILERRDVKPDEDLAGKAGGMVLVKGEGLYADPYGLLHEGRLSLAAAAGDPFAYPGAGGLYKRGSVRSLSTYSAAALQSDLEDSLYKAGAGGPLYGDGYGFRLPPSSPQKLADVSAPSGGPPPPHSPYSGPPSRGSPVRQSFRKDSGSSSVFAESPGGKARSTGASTAGAPPSELFPGPGERSLVGFGPPVPAKDTETRERMEAMEKQIASLTGLVQSALLRGSEPETPSEKIEGSNGAATPSAPVCGSGSRSSGATPVSGPPPPAVSSTPAGQPTAVSRLQMQMHLRGLQNSASDLRGQLQQLRKLQLQNQESVRALLKRTEAELSMRVSEAARRQEDPLQRQRTLVEEERLRYLNDEELITQQLNDLEKSVEKIQRDVAHNHRLVPGPELEEKALVLKQLGETLTELKAHFPGLQSKMRVVLRVEVEAVKFLKEEPQRLDGLLKRCRVVTDTLAQIRRQVDEGVWPPPNNLLNQSPKKVAAETDFSKGLDFEIPPPSPPLNLHELSGPAEGTPLTPKSGNPTKGLDAPSKRNMDKAVSVEAAERDWEEKRAALTQYSAKDINRLLEETQAELLKAIPDLDCASKTHPGPTPTPDHKPPKAPHGQKAAPRTEPSGRRGSDELTVPRYRTEKPSKSPPPPPPRRSFPSSHGLTTTRTGEVVVTSKKDSVFIKKAESEELEIQKPQVKLRRAVSEVVRPASTPPIMASAIKDEDDEERIIAELESGGGSVPPMKVVTPGASRLKAAQGPAGSPDKGKHGKQRTEYMRIQAQQQVRVGYQAPRPLEGCTPRLCYMPLS 8C9A2E630FB31C33 snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG DIP protein P60881 original sequence version: 105 DIP protein P60881 original sequence version: 40 DIP protein P60881 original sequence version: 106 DIP protein P60881 original sequence version: 78 DIP protein P60881 original sequence version: 75 DIP protein P60881 original sequence version: 100 DIP protein P60881 original sequence version: 120 DIP protein P60881 original sequence version: 131 DIP protein P60881 original sequence version: 93 FBED2B082A4CB6A6 p60879-1 Synaptosomal-associated protein 25 Snap25 Snap Super protein Synaptosomal-associated 25 kDa protein SNAP-25b protein protein mouse Mus musculus Mouse MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG FBED2B082A4CB6A6 snap25-snip-1 1 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association no figure snip-snap25-1 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey physical association physical association 2A Deletion studies revealed that SNIP specifically interacts with the Nbut not the C-terminal coiled-coil domain of SNAP-25 snap25-snip-2 4 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 2B snap25-snip-3 2 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 6 Co-localization occurs in filopodia, lamellipodia, neutitic processes and periphery of cell soma. Partial co-localization with actin cytoskeleton was shown using labeled F-actin. actin identity could not be mapped to sp.