IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science (0036-8075) 1999 imex curation Vassar R., Bennett BD., Babu-Khan S., Kahn S., Mendiaz EA., Denis P., Teplow DB., Ross S., Amarante P., Loeloff R., Luo Y., Fisher S., Fuller J., Edenson S., Lile J., Jarosinski MA., Biere AL., Curran E., Burgess T., Louis JC., Collins F., Treanor J., Rogers G., Citron M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease 293 cells were stable transfected with BACE-HA and transiently transfected with APP containing the Swedish mutation (KM670/671NL). For immunohistochemistry the cells were then costained with anti-HA antibodies and antibodies against the C terminal neoepitope generated upon B-secretase cleavage of APP (APPsBsw). mcitron@amgen.com 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Vassar R., Bennett BD., Babu-Khan S., Kahn S., Mendiaz EA., Denis P., Teplow DB., Ross S., Amarante P., Loeloff R., Luo Y., Fisher S., Fuller J., Edenson S., Lile J., Jarosinski MA., Biere AL., Curran E., Burgess T., Louis JC., Collins F., Treanor J., Rogers G., Citron M. Synapse - Interactions of proteins with an established role in the presynapse. 293 cells were stable transfected with BACE-HA and transiently transfected with APP containing the Swedish mutation (KM670/671NL). For immunohistochemistry the cells were then costained with anti-HA antibodies and antibodies against the C terminal neoepitope generated upon B-secretase cleavage of APP (APPsBsw). mcitron@amgen.com Accepted 2007-JUN-12 by ORCHARD. Science (0036-8075) 1999 Only protein-protein interactions imex curation Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science (0036-8075) 1999 imex curation Vassar R., Bennett BD., Babu-Khan S., Kahn S., Mendiaz EA., Denis P., Teplow DB., Ross S., Amarante P., Loeloff R., Luo Y., Fisher S., Fuller J., Edenson S., Lile J., Jarosinski MA., Biere AL., Curran E., Burgess T., Louis JC., Collins F., Treanor J., Rogers G., Citron M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Alzheimers - Interactions investigated in the context of Alzheimers disease 293 cells were stable transfected with BACE-HA and transiently transfected with APP containing the Swedish mutation (KM670/671NL). For immunohistochemistry the cells were then costained with anti-HA antibodies and antibodies against the C terminal neoepitope generated upon B-secretase cleavage of APP (APPsBsw). mcitron@amgen.com 27-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Vassar R., Bennett BD., Babu-Khan S., Kahn S., Mendiaz EA., Denis P., Teplow DB., Ross S., Amarante P., Loeloff R., Luo Y., Fisher S., Fuller J., Edenson S., Lile J., Jarosinski MA., Biere AL., Curran E., Burgess T., Louis JC., Collins F., Treanor J., Rogers G., Citron M. Synapse - Interactions of proteins with an established role in the presynapse. 293 cells were stable transfected with BACE-HA and transiently transfected with APP containing the Swedish mutation (KM670/671NL). For immunohistochemistry the cells were then costained with anti-HA antibodies and antibodies against the C terminal neoepitope generated upon B-secretase cleavage of APP (APPsBsw). mcitron@amgen.com Accepted 2007-JUN-12 by ORCHARD. 27-Oct-2007: Contacted by JYOTI. Science (0036-8075) 1999 Only protein-protein interactions imex curation a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 p56817-1 Beta-secretase 1 BACE-1A BAC-501 BACE1 BACE KIAA1149 Aspartyl protease 2 Beta-site amyloid precursor protein cleaving enzyme 1 Memapsin-2 Membrane-associated aspartic protease 2 protein protein human Homo sapiens Human MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK 377CE4C824ACEF05 app-bace1 1 3 unspecified role unspecified role neutral component neutral component P05067:p.Lys670_Met671delinsMetLeu mutation mutation certain certain sequence position certain certain certain sequence position certain KM ML Thu Jul 07 12:17:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys670met_asn671leu Fri Jul 22 11:03:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys670met,asn671leu' Wed May 02 15:44:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_met670-671met_leu' Tue Jun 30 18:32:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys670_Met671delinsMetLeu' 4 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig 4 Bace-1 isoform determined by examination of sequence in publication. app-bace1-1 2 3 enzyme target enzyme target substrate substrate neutral component neutral component P05067:p.Lys670_Met671delinsMetLeu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain KM ML Thu Jul 07 12:07:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys670met_asn671leu Fri Jul 22 11:00:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys670met,asn671leu' Wed May 02 16:17:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_met670-671met_leu' Tue Jun 30 18:30:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys670_Met671delinsMetLeu' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 enzyme enzyme neutral component neutral component luminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal Ig fusion fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. protein cleavage protein cleavage Fig 8 Bace-1 isoform determined by examination of sequence in publication.