IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Human mutation 2017 imex curation Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch Accepted 2020-JUL-21 AT 15:46 BST AT 15:46 BST by MGT Human mutation 2017 Only protein-protein interactions imex curation imex curation Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Human mutation 2017 imex curation Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch Accepted 2020-JUL-21 AT 15:46 BST AT 15:46 BST by MGT Human mutation 2017 Only protein-protein interactions imex curation imex curation Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Human mutation 2017 imex curation Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease Differential scanning fluorimetry:The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye (Sypro Orange) with affinity for hydrophobic parts of the protein, which are exposed as the protein unfolds sean.froese@kispi.uzh.ch Accepted 2020-JUL-21 AT 15:46 BST AT 15:46 BST by MGT Human mutation 2017 Only protein-protein interactions imex curation imex curation Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Human mutation 2017 imex curation Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight identificat confirmation by molecular weight weight identificat Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch Accepted 2020-JUL-21 AT 15:46 BST AT 15:46 BST by MGT Human mutation 2017 Only protein-protein interactions imex curation imex curation Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria. Human mutation 2017 imex curation Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease sean.froese@kispi.uzh.ch 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Plessl T., Bürer C., Lutz S., Yue WW., Baumgartner MR., Froese DS. Rare diseases - Interactions investigated in the context of Rare genetic disease The fluorophore MANT GTP (2'-(or-3')-O-(N-Methylanthraniloyl) Guanosine 5'-Triphosphate, Trisodium Salt) is a nucleotide analog modified on the ribose moiety. Its emitted fluorescent signal increases dramatically upon binding to a GTPase, enabling the detection of the interaction sean.froese@kispi.uzh.ch Accepted 2020-JUL-21 AT 15:46 BST AT 15:46 BST by MGT Human mutation 2017 Only protein-protein interactions imex curation imex curation guanosine 5'-[beta,gamma-imido]triphosphate guanosine 5'-[beta,gamma-imido]triphosphate 5'-Guanylic acid, monoanhydride with imidodiphosphoric acid 5'-guanylylimidodiphosphate GMP-PNP GMP-Pnp Gpp(NH)-p Gpp(NH)p Guanylyl imidodiphosphate 5'-O-(hydroxy{[hydroxy(phosphonoamino)phosphoryl]oxy}phosphoryl)guanosine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) muta_human Methylmalonyl-CoA mutase, mitochondrial MMUT MUT Methylmalonyl-CoA isomerase protein protein human Homo sapiens Human MLRAKNQLFLLSPHYLRQVKESSGSRLIQQRLLHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV B5909729C08B562F mmaa_human Methylmalonic aciduria type A protein, mitochondrial MMAA protein protein human Homo sapiens Human MPMLLPHPHQHFLKGLLRAPFRCYHFIFHSSTHLGSGIPCAQPFNSLGLHCTKWMLLSDGLKRKLCVQTTLKDHTEGLSDKEQRFVDKLYTGLIQGQRACLAEAITLVESTHSRKKELAQVLLQKVLLYHREQEQSNKGKPLAFRVGLSGPPGAGKSTFIEYFGKMLTERGHKLSVLAVDPSSCTSGGSLLGDKTRMTELSRDMNAYIRPSPTRGTLGGVTRTTNEAILLCEGAGYDIILIETVGVGQSEFAVADMVDMFVLLLPPAGGDELQGIKRGIIEMADLVAVTKSDGDLIVPARRIQAEYVSALKLLRKRSQVWKPKVIRISARSGEGISEMWDKMKDFQDLMLASGELTAKRRKQQKVWMWNLIQESVLEHFRTHPTVREQIPLLEQKVLIGALSPGLAADFLLKAFKSRD AD9EA19DDB8DEEF8 mant-gtp MANT-GTP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 gtp-mmaa-1 1 7 stimulator stimulator neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme neutral component neutral component purified purified Q8IVH4:p.Arg98Gly mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 17:28:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gly' Q8IVH4:p.Gly147Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 17:01:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly147Glu' Q8IVH4:p.Gly188Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:34:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly188Arg' Q8IVH4:p.Gly192Asp mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 15:45:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Arg196Gln mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 17:31:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg196Gln' Q8IVH4:p.Tyr207Cys mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 18:21:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr207Cys' Q8IVH4:p.Arg209Ser mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 16:29:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg209Ser' Q8IVH4:p.Gly218Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:02:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly218Glu' Q8IVH4:p.Val220Met mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 19:11:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val220Met' Q8IVH4:p.Thr243Asn mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 16:18:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr243Asn' Q8IVH4:p.Glu250Lys mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 16:41:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Lys' Q8IVH4:p.Gly274Ser mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain G S Tue Jun 30 18:06:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Q8IVH4:p.Lys276Glu mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 20:24:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys276Glu' Q8IVH4:p.Asp292Val mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 20:23:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.Arg359Gly mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 19:52:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg359Gly' binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) gtpase reaction gtpase reaction Supp. Table 3, Fig. 3 a/c, Table 2 MUT protein (UniProt identifier: P22033) mmaa-mmut-1 4 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified Q8IVH4:p.Gly192Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 20:18:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Arg196Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 20:14:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg196Gln' Q8IVH4:p.Glu250Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 18:20:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Lys' Q8IVH4:p.Gly274Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G S Tue Jun 30 17:00:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Q8IVH4:p.Lys276Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 18:49:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys276Glu' Q8IVH4:p.Arg359Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 16:51:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg359Gly' Q8IVH4:p.Arg98Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 17:23:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gly' Q8IVH4:p.Gly188Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:36:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly188Arg' Q8IVH4:p.Gly218Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 20:19:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly218Glu' Q8IVH4:p.Gly147Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 20:07:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly147Glu' Q8IVH4:p.Tyr207Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 19:04:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr207Cys' Q8IVH4:p.Arg209Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 17:11:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg209Ser' Q8IVH4:p.Val220Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 16:37:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val220Met' Q8IVH4:p.Asp292Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 18:15:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.Thr243Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T N binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4a, S3, S4 GMPPNP (CHEBI:78408) - non-hydrolysable analogue of GTP Mg2+ mmaa-mmut-2 2 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q8IVH4:p.Gly192Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:02:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Thr243Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 18:13:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr243Asn' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4b, Supp. Figure S5 GMPPNP (CHEBI:78408) Mg2+ gtp-mmaa-2 5 8 unspecified role unspecified role neutral component neutral component purified purified Q8IVH4:p.Asp292Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 18:59:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.[Lys290Ala;Asp292Val] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V certain certain sequence position certain certain certain sequence position certain K A Mon Dec 16 10:42:25 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp292Val;Lys290Ala]' Tue Jun 30 19:52:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys290Ala;Asp292Val]' binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. S1 gdp-mmaa-1 3 10 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified Q8IVH4:p.Tyr207Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 16:49:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr207Cys' Q8IVH4:p.Arg209Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 20:11:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg209Ser' Q8IVH4:p.Val220Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 17:30:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val220Met' Q8IVH4:p.Gly218Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 15:52:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly218Glu' Q8IVH4:p.Thr243Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 18:15:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr243Asn' Q8IVH4:p.Glu250Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 18:47:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Lys' Q8IVH4:p.[Lys290Ala;Asp292Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 15:54:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Wed Sep 16 10:25:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q8IVH4:p.[Lys290Ala;Asp292Ala]' Q8IVH4:p.Asp292Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 18:44:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.Arg98Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 16:48:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gly' Q8IVH4:p.Gly147Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 16:45:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly147Glu' Q8IVH4:p.Gly188Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:27:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly188Arg' Q8IVH4:p.Gly192Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:26:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Arg196Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 15:44:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg196Gln' Q8IVH4:p.Gly274Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G S Tue Jun 30 20:18:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Q8IVH4:p.Lys276Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 19:16:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys276Glu' Q8IVH4:p.Arg359Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 16:07:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg359Gly' binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2a, 2c, Suppl. table 2 gtp-mmaa-5 1 11 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme neutral component neutral component purified purified Q8IVH4:p.Arg98Gly mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 17:28:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gly' Q8IVH4:p.Gly147Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 17:01:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly147Glu' Q8IVH4:p.Gly188Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:34:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly188Arg' Q8IVH4:p.Gly192Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 15:45:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Arg196Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 17:31:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg196Gln' Q8IVH4:p.Tyr207Cys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 18:21:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr207Cys' Q8IVH4:p.Arg209Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 16:29:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg209Ser' Q8IVH4:p.Gly218Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 18:02:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly218Glu' Q8IVH4:p.Val220Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 19:11:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val220Met' Q8IVH4:p.Thr243Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 16:18:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr243Asn' Q8IVH4:p.Glu250Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 16:41:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Lys' Q8IVH4:p.Gly274Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain G S Tue Jun 30 18:06:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Q8IVH4:p.Lys276Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 20:24:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys276Glu' Q8IVH4:p.Asp292Val mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 20:23:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.Arg359Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 19:52:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg359Gly' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8IVH4:p.[Lys290Ala;Asp292Ala] mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain D A Fri Sep 18 23:35:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q8IVH4:p.Lys290Ala;Asp292Ala' ecoli Escherichia coli (strain K12) gtpase reaction gtpase reaction Fig. 3b, Table 2 guanosine 5'_[beta,gamma_imido]triphosphate-mmaa-2 3 6 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified Q8IVH4:p.Tyr207Cys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y C Tue Jun 30 16:49:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr207Cys' Q8IVH4:p.Arg209Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 20:11:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg209Ser' Q8IVH4:p.Val220Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Tue Jun 30 17:30:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val220Met' Q8IVH4:p.Gly218Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 15:52:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly218Glu' Q8IVH4:p.Thr243Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T N Tue Jun 30 18:15:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr243Asn' Q8IVH4:p.Glu250Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 18:47:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Lys' Q8IVH4:p.[Lys290Ala;Asp292Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 15:54:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Wed Sep 16 11:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q8IVH4:p.[Lys290Ala;Asp292Ala]' Q8IVH4:p.Asp292Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 18:44:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp292Val' Q8IVH4:p.Arg98Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 16:48:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg98Gly' Q8IVH4:p.Gly147Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G E Tue Jun 30 16:45:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly147Glu' Q8IVH4:p.Gly188Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:27:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly188Arg' Q8IVH4:p.Gly192Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:26:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Asp' Q8IVH4:p.Arg196Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Tue Jun 30 15:44:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg196Gln' Q8IVH4:p.Gly274Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G S Tue Jun 30 20:18:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly274Ser' Q8IVH4:p.Lys276Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Tue Jun 30 19:16:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys276Glu' Q8IVH4:p.Arg359Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 16:07:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg359Gly' binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2b/c, Suppl. table 2