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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
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        <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>X-ray structure of a bifunctional protein kinase in complex with its protein substrate HPr.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="12359875" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1989613" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">X-ray structure of a bifunctional protein kinase in complex with its protein substrate HPr.</attribute>
            <attribute name="journal" nameAc="MI:0885">Proc. Natl. Acad. Sci. U.S.A. (0027-8424)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2002</attribute>
            <attribute name="author-list" nameAc="MI:0636">Fieulaine S., Morera S., Poncet S., Mijakovic I., Galinier A., Janin J., Deutscher J., Nessler S.</attribute>
            <attribute name="dataset" nameAc="MI:0875">PDBe - Data obtained from the Protein Data Bank Europe</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="12359875" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Fieulaine S., Morera S., Poncet S., Mijakovic I., Galinier A., Janin J., Deutscher J., Nessler S.</attribute>
          <attribute name="dataset" nameAc="MI:0875">PDBe - Data obtained from the Protein Data Bank Europe</attribute>
          <attribute name="accepted"/>
          <attribute name="journal" nameAc="MI:0885">Proc. Natl. Acad. Sci. U.S.A. (0027-8424)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2002</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>pthp_bacsu</shortLabel>
          <fullName>Phosphocarrier protein HPr</fullName>
          <alias type="gene name" typeAc="MI:0301">ptsH</alias>
          <alias type="locus name" typeAc="MI:0305">BSU13900</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Histidine-containing protein</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P08877" version="SP_92" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="CAB13263" version="SP_191" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1034482" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_389273.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001020"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000032"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002114"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1JEM"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1KKL"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1KKM"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SPH"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2FEP"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2HID"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2HPR"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3OQM"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3OQN"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3OQO"/>
          <secondaryRef db="ensemblbacteria" id="BSU_13900" version="SP_191" refType="gene"/>
          <secondaryRef db="ensemblbacteria" id="CAB13263" version="SP_191" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005737" version="SP_191"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009401" version="SP_191"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043610" version="SP_191"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_003154654.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR035895"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="224308">
          <names>
            <shortLabel>bacsu</shortLabel>
            <fullName>Bacillus subtilis</fullName>
          </names>
        </organism>
        <sequence>MAQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSEGLGE</sequence>
        <attributeList>
          <attribute name="crc64">2B13DBD63DD636A9</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>hprk_lacca</shortLabel>
          <fullName>HPr kinase/phosphorylase</fullName>
          <alias type="gene name" typeAc="MI:0301">hprK</alias>
          <alias type="gene name synonym" typeAc="MI:0302">HPr(Ser) kinase/phosphorylase</alias>
          <alias type="gene name synonym" typeAc="MI:0302">HPr kinase/phosphatase</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q9RE09" version="SP_37" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1034475" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR003755"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011104"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011126"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1JB1"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1KKL"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1KKM"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2QMH"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027417" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000155" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000287" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004674" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004712" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005524" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005975" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006109" version="SP_107"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_003564222.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR028979"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="1582">
          <names>
            <shortLabel>lacca</shortLabel>
            <fullName>Lactobacillus casei</fullName>
          </names>
        </organism>
        <sequence>MADSVTVRQLVKATKLEVYSGEEYLDQRQVVLSDISRPGLELTGYFNYYPHERIQLFGRTEISFARNMSSEERLLILKRMATEDTPAFLVSRGLEAPAEMITAATAAHIPVLGSRLPTTRLSSLITEYLDSQLAERRSMHGVLVDIYGLGVLITGDSGVGKSETALELVQRGHRLIADDRVDVYQQDEQTIVGAAPPILSHLLEIRGLGIIDVMNLFGAGAVREDTTISLIVHLENWTPDKTFDRLGSGEQTQLIFDVPVPKITVPVKVGRNLAIIIEVAAMNFRAKSMGYDATKTFEKNLNHLIEHNEETDQNSSGDK</sequence>
        <attributeList>
          <attribute name="crc64">96D27AE3D31132E8</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>hprk-ptsh-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="rcsb pdb" dbAc="MI:0460" id="1KKM" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1034497" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="6">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-1034502" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="2"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
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                      </xref>
                    </endStatus>
                    <end position="88"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="3"/>
          </participant>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="8">
                <names>
                  <shortLabel>region</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-1034499" refType="identity" refTypeAc="MI:0356"/>
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                <featureType>
                  <names>
                    <shortLabel>sufficient to bind</shortLabel>
                    <fullName>sufficient binding region</fullName>
                    <alias type="synonym" typeAc="MI:1041">sufficient to bind</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0442" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608899" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
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                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="128"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
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                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="319"/>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="3"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="assembly">dodecameric, multimeric</attribute>
          <attribute name="3d-r-factors" nameAc="MI:0631">working 80.0%, free 74.300%</attribute>
          <attribute name="3d-resolution" nameAc="MI:0632">2.8A</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>