IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer (0020-7136) 2009 imex curation Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. yeonsooseo@kaist.ac.kr, misun@kribb.re.kr 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells orchard Only protein-protein interactions human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Kim DM., Chung KS., Choi SJ., Jung YJ., Park SK., Han GH., Ha JS., Song KB., Choi NS., Kim HM., Won M., Seo YS. Apoptosis - Interactions involving proteins with a function related to apoptosis yeonsooseo@kaist.ac.kr, misun@kribb.re.kr Accepted 2009-Not a month!-4 by ORCHARD. 23-Feb-2010: Contacted by JYOTI. pGAL4AD-cDNA library from HeLa cells Int. J. Cancer (0020-7136) 2009 orchard Only protein-protein interactions imex curation map1a_human Microtubule-associated protein 1A MAP1A Proliferation-related protein p80 MAP1L protein protein human Homo sapiens Human MDGVAEFSEYVSETVDVPSPFDLLEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGFNILVDGGSDRKSCFWKLVRHLDRIDSVLLTHIGADNLPGINGLLQRKVAELEEEQSQGSSSYSDWVKNLISPELGVVFFNVPEKLRLPDASRKAKRSIEEACLTLQHLNRLGIQAEPLYRVVSNTIEPLTLFHKMGVGRLDMYVLNPVKDSKEMQFLMQKWAGNSKAKTGIVLPNGKEAEISVPYLTSITALVVWLPANPTEKIVRVLFPGNAPQNKILEGLEKLRHLDFLRYPVATQKDLASGAVPTNLKPSKIKQRADSKESLKATTKTAVSKLAKREEVVEEGAKEARSELAKELAKTEKKAKESSEKPPEKPAKPERVKTESSEALKAEKRKLIKDKVGKKHLKEKISKLEEKKDKEKKEIKKERKELKKDEGRKEEKKDAKKEEKRKDTKPELKKISKPDLKPFTPEVRKTLYKAKVPGRVKIDRSRAIRGEKELSSEPQTPPAQKGTVPLPTISGHRELVLSSPEDLTQDFEEMKREERALLAEQRDTGLGDKPFPLDTAEEGPPSTAIQGTPPSVPGLGQEEHVMKEKELVPEVPEEQGSKDRGLDSGAETEEEKDTWEEKKQREAERLPDRTEAREESEPEVKEDVIEKAELEEMEEVHPSDEEEEDATKAEGFYQKHMQEPLKVTPRSREAFGGRELGLQGKAPEKETSLFLSSLTTPAGATEHVSYIQDETIPGYSETEQTISDEEIHDEPEERPAPPRFHTSTYDLPGPEGAGPFEASQPADSAVPATSGKVYGTPETELTYPTNIVAAPLAEEEHVSSATSITECDKLSSFATSVAEDQSVASLTAPQTEETGKSSLLLDTVTSIPSSRTEATQGLDYVPSAGTISPTSSLEEDKGFKSPPCEDFSVTGESEKRGEIIGKGLSGERAVEEEEEETANVEMSEKLCSQYGTPVFSAPGHALHPGEPALGEAEERCLSPDDSTVKMASPPPSGPPSATHTPFHQSPVEEKSEPQDFQEADSWGDTKRTPGVGKEDAAEETVKPGPEEGTLEKEEKVPPPRSPQAQEAPVNIDEGLTGCTIQLLPAQDKAIVFEIMEAGEPTGPILGAEALPGGLRTLPQEPGKPQKDEVLRYPDRSLSPEDAESLSVLSVPSPDTANQEPTPKSPCGLTEQYLHKDRWPEVSPEDTQSLSLSEESPSKETSLDVSSKQLSPESLGTLQFGELNLGKEEMGHLMQAEDTSHHTAPMSVPEPHAATASPPTDGTTRYSAQTDITDDSLDRKSPASSFSHSTPSGNGKYLPGAITSPDEHILTPDSSFSKSPESLPGPALEDIAIKWEDKVPGLKDRTSEQKKEPEPKDEVLQQKDKTLEHKEVVEPKDTAIYQKDEALHVKNEAVKQQDKALEQKGRDLEQKDTALEQKDKALEPKDKDLEEKDKALEQKDKIPEEKDKALEQKDTALEQKDKALEPKDKDLEQKDRVLEQKEKIPEEKDKALDQKVRSVEHKAPEDTVAEMKDRDLEQTDKAPEQKHQAQEQKDKVSEKKDQALEQKYWALGQKDEALEQNIQALEENHQTQEQESLVQEDKTRKPKMLEEKSPEKVKAMEEKLEALLEKTKALGLEESLVQEGRAREQEEKYWRGQDVVQEWQETSPTREEPAGEQKELAPAWEDTSPEQDNRYWRGREDVALEQDTYWRELSCERKVWFPHELDGQGARPHYTEERESTFLDEGPDDEQEVPLREHATRSPWASDFKDFQESSPQKGLEVERWLAESPVGLPPEEEDKLTRSPFEIISPPASPPEMVGQRVPSAPGQESPIPDPKLMPHMKNEPTTPSWLADIPPWVPKDRPLPPAPLSPAPGPPTPAPESHTPAPFSWGTAEYDSVVAAVQEGAAELEGGPYSPLGKDYRKAEGEREEEGRAEAPDKSSHSSKVPEASKSHATTEPEQTEPEQREPTPYPDERSFQYADIYEQMMLTGLGPACPTREPPLGAAGDWPPCLSTKEAAAGRNTSAEKELSSPISPKSLQSDTPTFSYAALAGPTVPPRPEPGPSMEPSLTPPAVPPRAPILSKGPSPPLNGNILSCSPDRRSPSPKESGRSHWDDSTSDSELEKGAREQPEKEAQSPSPPHPIPMGSPTLWPETEAHVSPPLDSHLGPARPSLDFPASAFGFSSLQPAPPQLPSPAEPRSAPCGSLAFSGDRALALAPGPPTRTRHDEYLEVTKAPSLDSSLPQLPSPSSPGAPLLSNLPRPASPALSEGSSSEATTPVISSVAERFSPSLEAAEQESGELDPGMEPAAHSLWDLTPLSPAPPASLDLALAPAPSLPGDMGDGILPCHLECSEAATEKPSPFQVPSEDCAANGPTETSPNPPGPAPAKAENEEAAACPAWERGAWPEGAERSSRPDTLLSPEQPVCPAGGSGGPPSSASPEVEAGPQGCATEPRPHRGELSPSFLNPPLPPSIDDRDLSTEEVRLVGRGGRRRVGGPGTTGGPCPVTDETPPTSASDSGSSQSDSDVPPETEECPSITAEAALDSDEDGDFLPVDKAGGVSGTHHPRPGHDPPPLPQPDPRPSPPRPDVCMADPEGLSSESGRVERLREKEKVQGRVGRRAPGKAKPASPARRLDLRGKRSPTPGKGPADRASRAPPRPRSTTSQVTPAEEKDGHSPMSKGLVNGLKAGPMALSSKGSSGAPVYVDLAYIPNHCSGKTADLDFFRRVRASYYVVSGNDPANGEPSRAVLDALLEGKAQWGENLQVTLIPTHDTEVTREWYQQTHEQQQQLNVLVLASSSTVVMQDESFPACKIEF DIP protein P78559 original sequence version: 128 94733902420F1FFE ngef_human Ephexin-1 Eph-interacting exchange protein Neuronal guanine nucleotide exchange factor NGEF protein protein human Homo sapiens Human METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRMPPCRTAMQTDPGAQEMSESSSTPGNGATPEEWPALADSPTTLTEALRMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIEDNTNGSPASEDTPEEEEEEEEEEEPASPPERKTLPQICLLSNPHSRFNLWQDLPEIRSSGVLEILQPEEIKLQEAMFELVTSEASYYKSLNLLVSHFMENERIRKILHPSEAHILFSNVLDVLAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPFSSFLILPFQRITRLKLLVQNILKRVEERSERECTALDAHKELEMVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFHEIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRVEELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTDDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ 82A4CA71D4E7AE6E eif1b_human Eukaryotic translation initiation factor 1b Protein translation factor SUI1 homolog GC20 EIF1B protein protein human Homo sapiens Human MSTIQNLQSFDPFADATKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLLEVGIVKEEQLKVHGF 4522A8B692654FE1 rhob_human Rho-related GTP-binding protein RhoB Rho cDNA clone 6 RHOB ARH6 ARHB protein protein human Homo sapiens Human MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTELARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQKRYGSQNGCINCCKVL CCE6FD53AE00CD83 arhg1_human Rho guanine nucleotide exchange factor 1 ARHGEF1 115 kDa guanine nucleotide exchange factor Sub1.5 protein protein human Homo sapiens Human MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT 1E773D041652190D ppig_human Peptidyl-prolyl cis-trans isomerase G PPIG Cyclophilin G Clk-associating RS-cyclophilin CASP10 Rotamase G protein protein human Homo sapiens Human MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRRYRTPSRSRSRDRFRRSETPPHWRQEMQRAQRMRVSSGERWIKGDKSELNEIKENQRSPVRVKERKITDHRNVSESPNRKNEKEKKVKDHKSNSKERDIRRNSEKDDKYKNKVKKRAKSKSRSKSKEKSKSKERDSKHNRNEEKRMRSRSKGRDHENVKEKEKQSDSKGKDQERSRSKEKSKQLESKSNEHDHSKSKEKDRRAQSRSRECDITKGKHSYNSRTRERSRSRDRSRRVRSRTHDRDRSRSKEYHRYREQEYRRRGRSRSRERRTPPGRSRSKDRRRRRRDSRSSEREESQSRNKDKYRNQESKSSHRKENSESEKRMYSKSRDHNSSNNSREKKADRDQSPFSKIKQSSQDNELKSSMLKNKEDEKIRSSVEKENQKSKGQENDHVHEKNKKFDHESSPGTDEDKSG D4884808D8060CD6 sera_human D-3-phosphoglycerate dehydrogenase PHGDH PGDH3 2-oxoglutarate reductase Malate dehydrogenase protein protein human Homo sapiens Human MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF C58EB72275C45B35 dut_human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial DUT dUTP pyrophosphatase protein protein human Homo sapiens Human MTPLCPRPALCYHFLTSLLRSAMQNARGARQRAEAAVLSGPGPPLGRAAQHGIPRPLSSAGRLSQGCRGASTVGAAGWKGELPKAGGSPAPGPETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN 8E2EBC9ED5B0FAD2 nek2_human Serine/threonine-protein kinase Nek2 NEK2A NimA-like protein kinase 1 HSPK 21 NEK2 NLK1 Never in mitosis A-related kinase 2 protein protein human Homo sapiens Human MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR D33A37778ABB6D9E praf2_human PRA1 family protein 2 PRAF2 JM4 protein protein human Homo sapiens Human MSEVRLPPLRALDDFVLGSARLAAPDPCDPQRWCHRVINNLLYYQTNYLLCFGIGLALAGYVRPLHTLLSALVVAVALGVLVWAAETRAAVRRCRRSHPAACLAAVLAVGLLVLWVAGGACTFLFSIAGPVLLILVHASLRLRNLKNKIENKIESIGLKRTPMGLLLEALGQEQEAGS E62695D7DC40550A srtd1_human SERTA domain-containing protein 1 Transcriptional regulator interacting with the PHD-bromodomain 1 CDK4-binding protein p34SEI1 SERTAD1 SEI1 TRIPBR1 protein protein human Homo sapiens Human MLSKGLKRKREEEEEKEPLAVDSWWLDPGHTAVAQAPPAVASSSLFDLSVLKLHHSLQQSEPDLRHLVLVVNTLRRIQASMAPAAALPPVPSPPAAPSVADNLLASSDAALSASMASLLEDLSHIEGLSQAPQPLADEGPPGRSIGGAAPSLGALDLLGPATGCLLDDGLEGLFEDIDTSMYDNELWAPASEGLKPGPEDGPGKEEAPELDEAELDYLMDVLVGTQALERPPGPGR 8659505783FBF50B gdir1_human Rho GDP-dissociation inhibitor 1 Rho-GDI alpha ARHGDIA GDIA1 protein protein human Homo sapiens Human MAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVVVTGLTLVCSSAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGPRAEEYEFLTPVEEAPKGMLARGSYSIKSRFTDDDKTDHLSWEWNLTIKKDWKD 59CB6F42E3B3BCCA ndkb_human Nucleoside diphosphate kinase B nm23-H2 C-myc purine-binding transcription factor PUF NME2 NM23B Histidine protein kinase NDKB protein protein human Homo sapiens Human MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE DIP protein P22392 original sequence version: 182 1A5C3F84D7AD272C bacd2_human BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 BACURD2 BTB/POZ domain-containing protein TNFAIP1 Protein B12 TNFAIP1 EDP1 Tumor necrosis factor, alpha-induced protein 1, endothelial protein protein human Homo sapiens Human MSGDTCLCPASGAKPKLSGFKGGGLGNKYVQLNVGGSLYYTTVRALTRHDTMLKAMFSGRMEVLTDKEGWILIDRCGKHFGTILNYLRDDTITLPQNRQEIKELMAEAKYYLIQGLVNMCQSALQDKKDSYQPVCNIPIITSLKEEERLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRSRSQASPSEDEETFELRDRVRRIHVKRYSTYDDRQLGHQSTHRD D20B810A00507DCF fbn1_human Fibrillin-1 FBN1 FBN protein protein human Homo sapiens Human MRRGRLLEIALGFTVLLASYTSHGADANLEAGNVKETRASRAKRRGGGGHDALKGPNVCGSRYNAYCCPGWKTLPGGNQCIVPICRHSCGDGFCSRPNMCTCPSGQIAPSCGSRSIQHCNIRCMNGGSCSDDHCLCQKGYIGTHCGQPVCESGCLNGGRCVAPNRCACTYGFTGPQCERDYRTGPCFTVISNQMCQGQLSGIVCTKTLCCATVGRAWGHPCEMCPAQPHPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECKCPAGHKLNEVSQKCEDIDECSTIPGICEGGECTNTVSSYFCKCPPGFYTSPDGTRCIDVRPGYCYTALTNGRCSNQLPQSITKMQCCCDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRPEYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRYLCQNGRCIPTPGSYRCECNKGFQLDLRGECIDVDECEKNPCAGGECINNQGSYTCQCRAGYQSTLTRTECRDIDECLQNGRICNNGRCINTDGSFHCVCNAGFHVTRDGKNCEDMDECSIRNMCLNGMCINEDGSFKCICKPGFQLASDGRYCKDINECETPGICMNGRCVNTDGSYRCECFPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCIKPLFGAVTKSECCCASTEYAFGEPCQPCPAQNSAEYQALCSSGPGMTSAGSDINECALDPDICPNGICENLRGTYKCICNSGYEVDSTGKNCVDINECVLNSLLCDNGQCRNTPGSFVCTCPKGFIYKPDLKTCEDIDECESSPCINGVCKNSPGSFICECSSESTLDPTKTICIETIKGTCWQTVIDGRCEININGATLKSQCCSSLGAAWGSPCTLCQVDPICGKGYSRIKGTQCEDIDECEVFPGVCKNGLCVNTRGSFKCQCPSGMTLDATGRICLDIRLETCFLRYEDEECTLPIAGRHRMDACCCSVGAAWGTEECEECPMRNTPEYEELCPRGPGFATKEITNGKPFFKDINECKMIPSLCTHGKCRNTIGSFKCRCDSGFALDSEERNCTDIDECRISPDLCGRGQCVNTPGDFECKCDEGYESGFMMMKNCMDIDECQRDPLLCRGGVCHNTEGSYRCECPPGHQLSPNISACIDINECELSAHLCPNGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETFCTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLCYDGFMASEDMKTCVDVNECDLNPNICLSGTCENTKGSFICHCDMGYSGKKGKTGCTDINECEIGAHNCGKHAVCTNTAGSFKCSCSPGWIGDGIKCTDLDECSNGTHMCSQHADCKNTMGSYRCLCKEGYTGDGFTCTDLDECSENLNLCGNGQCLNAPGGYRCECDMGFVPSADGKACEDIDECSLPNICVFGTCHNLPGLFRCECEIGYELDRSGGNCTDVNECLDPTTCISGNCVNTPGSYICDCPPDFELNPTRVGCVDTRSGNCYLDIRPRGDNGDTACSNEIGVGVSKASCCCSLGKAWGTPCEMCPAVNTSEYKILCPGGEGFRPNPITVILEDIDECQELPGLCQGGKCINTFGSFQCRCPTGYYLNEDTRVCDDVNECETPGICGPGTCYNTVGNYTCICPPDYMQVNGGNNCMDMRRSLCYRNYYADNQTCDGELLFNMTKKMCCCSYNIGRAWNKPCEQCPIPSTDEFATLCGSQRPGFVIDIYTGLPVDIDECREIPGVCENGVCINMVGSFRCECPVGFFYNDKLLVCEDIDECQNGPVCQRNAECINTAGSYRCDCKPGYRFTSTGQCNDRNECQEIPNICSHGQCIDTVGSFYCLCHTGFKTNDDQTMCLDINECERDACGNGTCRNTIGSFNCRCNHGFILSHNNDCIDVDECASGNGNLCRNGQCINTVGSFQCQCNEGYEVAPDGRTCVDINECLLEPRKCAPGTCQNLDGSYRCICPPGYSLQNEKCEDIDECVEEPEICALGTCSNTEGSFKCLCPEGFSLSSSGRRCQDLRMSYCYAKFEGGKCSSPKSRNHSKQECCCALKGEGWGDPCELCPTEPDEAFRQICPYGSGIIVGPDDSAVDMDECKEPDVCKHGQCINTDGSYRCECPFGYILAGNECVDTDECSVGNPCGNGTCKNVIGGFECTCEEGFEPGPMMTCEDINECAQNPLLCAFRCVNTYGSYECKCPVGYVLREDRRMCKDEDECEEGKHDCTEKQMECKNLIGTYMCICGPGYQRRPDGEGCVDENECQTKPGICENGRCLNTRGSYTCECNDGFTASPNQDECLDNREGYCFTEVLQNMCQIGSSNRNPVTKSECCCDGGRGWGPHCEICPFQGTVAFKKLCPHGRGFMTNGADIDECKVIHDVCRNGECVNDRGSYHCICKTGYTPDITGTSCVDLNECNQAPKPCNFICKNTEGSYQCSCPKGYILQEDGRSCKDLDECATKQHNCQFLCVNTIGGFTCKCPPGFTQHHTSCIDNNECTSDINLCGSKGICQNTPGSFTCECQRGFSLDQTGSSCEDVDECEGNHRCQHGCQNIIGGYRCSCPQGYLQHYQWNQCVDENECLSAHICGGASCHNTLGSYKCMCPAGFQYEQFSGGCQDINECGSAQAPCSYGCSNTEGGYLCGCPPGYFRIGQGHCVSGMGMGRGNPEPPVSGEMDDNSLSPEACYECKINGYPKRGRKRRSTNETDASNIEDQSETEANVSLASWDVEKTAIFAFNISHVSNKVRILELLPALTTLTNHNRYLIESGNEDGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDKDYLSGELGDNLKMKIQVLLH DIP protein P35555 original sequence version: 111 DIP protein P35555 original sequence version: 169 501258AF1756B9F7 bacd3_human BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3 BTB/POZ domain-containing protein KCTD10 Potassium channel tetramerization domain-containing protein 10 KCTD10 ULR061 MSTP028 protein protein human Homo sapiens Human MEEMSGESVVSSAVPAAATRTTSFKGTSPSSKYVKLNVGGALYYTTMQTLTKQDTMLKAMFSGRMEVLTDSEGWILIDRCGKHFGTILNYLRDGAVPLPESRREIEELLAEAKYYLVQGLVEECQAALQNKDTYEPFCKVPVITSSKEEQKLIATSNKPAVKLLYNRSNNKYSYTSNSDDNMLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIYEETLNILLYEAQDGRGPDNALLEATGGAAGRSHHLDEDEERERIERVRRIHIKRPDDRAHLHQ 199AF07A30D5BCA6 fbx42_human F-box only protein 42 FBXO42 FBX42 JFK KIAA1332 Just one F-box and Kelch domain-containing protein protein protein human Homo sapiens Human MASSSDSEDDSFMAVDQEETVLEGTMDQDEEPHPVLEAEETRHNRSMSELPEEVLEYILSFLSPYQEHKTAALVCKQWYRLIKGVAHQCYHGFMKAVQEGNIQWESRTYPYPGTPITQRFSHSACYYDANQSMYVFGGCTQSSCNAAFNDLWRLDLNSKEWIRPLASGSYPSPKAGATLVVYKDLLVLFGGWTRPSPYPLHQPERFFDEIHTYSPSKNWWNCIVTTHGPPPMAGHSSCVIDDKMIVFGGSLGSRQMSNDVWVLDLEQWAWSKPNISGPSPHPRGGQSQIVIDDATILILGGCGGPNALFKDAWLLHMHSGPWAWQPLKVENEEHGAPELWCHPACRVGQCVVVFSQAPSGRAPLSPSLNSRPSPISATPPALVPETREYRSQSPVRSMDEAPCVNGRWGTLRPRAQRQTPSGSREGSLSPARGDGSPILNGGSLSPGTAAVGGSSLDSPVQAISPSTPSAPEGYDLKIGLSLAPRRGSLPDQKDLRLGSIDLNWDLKPASSSNPMDGMDNRTVGGSMRHPPEQTNGVHTPPHVASALAGAVSPGALRRSLEAIKAMSSKGPSASAALSPPLGSSPGSPGSQSLSSGETVPIPRPGPAQGDGHSLPPIARRLGHHPPQSLNVGKPLYQSMNCKPMQMYVLDIKDTKEKGRVKWKVFNSSSVVGPPETSLHTVVQGRGELIIFGGLMDKKQNVKYYPKTNALYFVRAKR 938D0A018FC3DCBD nqo2_human Ribosyldihydronicotinamide dehydrogenase [quinone] NQO2 NMOR2 NRH dehydrogenase [quinone] 2 NRH:quinone oxidoreductase 2 Quinone reductase 2 protein protein human Homo sapiens Human MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ DIP protein P16083 original sequence version: 186 FE1F4D577517B972 rhob-tnap1-1 1 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-kcd10 1 10 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey physical association physical association rhob-nek2 1 10 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association rhob-ndkb 1 19 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-srtd1 1 10 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey physical association physical association rhob-fbn1 1 21 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-phgdh 1 10 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association rhob-nqo2 4 24 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-ngef 4 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-arhg1 4 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association rhob-gdir1 4 18 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-dut 4 10 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association rhob-ppig 4 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-eif1b 4 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-praf2 4 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-fbx42 4 23 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association rhob-map1a 4 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association rhob-tnap1-2 3 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role prey prey physical association physical association Fig. 2b TNFAIP1 coimmunoprecipitated with RhoB was detected by western blot using anti-TNFAIP1 antibody. rhob-tnap1-3 5 10 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 20 unspecified role unspecified role bait bait region 6X His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) physical association physical association Fig. 2c When His-TNFAIP1 protein was purified on His-bind resins after incubation of mixture with His-bind resins, only GST-RhoB was copurified with His-TNFAIP together as shown in Western blot rhob-tnap1-4 6 10 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role neutral component neutral component c-terminal rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 2d a, b Localization of RhoB and TNFAIP1 was observed by confocal fluorescence microscopy. rhob-tnap1-5 2 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 2a Yeast two-hybrid analysis demonstrated an interaction of RhoB with TNFAIP1 following Ura3, Ade2 and LacZ reporter assays (Fig. 2a).