IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. The Journal of biological chemistry 2014 imex curation Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-t-cell Homo sapiens t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease Regulatory T cells (CD4+, CD25+, Cd127 low) in vitro expanded by IL-2/anti-CD3/CD28 exposure. The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn Accepted 2016-FEB-03 AT 09:06 GMT AT 09:06 GMT by ORCHARD The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. The Journal of biological chemistry 2014 imex curation Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease !!Ref 14 supplement describes Flag-FOXP3 - but althought the myc tag is N-terminal originally I did not assumed the FLAG/HA tags will be, as the vector used (pIRES-puromycin) can have both N and C terminal tags. However under Fig 4 the text stated that the FOXP3 FLAG tag is N-terminal and presumably the HA- PIM1 as well. The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn Accepted 2016-FEB-03 AT 09:06 GMT AT 09:06 GMT by ORCHARD The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. The Journal of biological chemistry 2014 imex curation Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-jurkat Homo sapiens jurkat Human T cell leukemia ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn Accepted 2016-FEB-03 AT 09:06 GMT AT 09:06 GMT by ORCHARD The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation. The Journal of biological chemistry 2014 imex curation Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Li Z., Lin F., Zhuo C., Deng G., Chen Z., Yin S., Gao Z., Piccioni M., Tsun A., Cai S., Zheng SG., Zhang Y., Li B. IBD - Inflammatory bowel disease The authors have stated that PIM1 constructs were constructed with human PIM1 cDNA. sh_zhangyu@hotmail.com, binli@sibs.ac.cn Accepted 2016-FEB-03 AT 09:06 GMT AT 09:06 GMT by ORCHARD The Journal of biological chemistry 2014 Only protein-protein interactions imex curation imex curation il2_human_gene Human IL-2 gene IL2 gene gene human Homo sapiens Human q9bzs1-1 Forkhead box protein P3 FOXP3 IPEX Scurfin JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP DIP protein Q9BZS1-1 original sequence version: 139 DIP protein Q9BZS1-1 original sequence version: 135 91737C3CEA665A15 p11309-1 Serine/threonine-protein kinase pim-1 Pim-1 PIM1 protein protein human Homo sapiens Human MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. 18C421B7CFF87818 foxp3-pim1-1 2 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BZS1-1:p.Ser422Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:34:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser422ala' Tue Jun 30 19:12:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser422Ala' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11309-1:p.Lys67Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:27:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67met' Tue Oct 30 11:33:51 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Met' Tue Jun 30 16:03:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Met' physical association physical association 1 C pim1-foxp3-1 4 6 enzyme target enzyme target substrate substrate neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser422 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecoli Escherichia coli (strain K12) 7 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P11309-1:p.Lys67Met mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:56:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67met' Tue Oct 30 13:23:52 GMT 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Met' Tue Jun 30 15:38:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Met' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1 E P11309-2 inhibits activity of Q9BZS1-1 pim1-foxp3-2 2 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BZS1-1:p.Ser422Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:41:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser422ala' Tue Jun 30 15:37:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser422Ala' Q9BZS1-1:p.Ser418Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:48:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418asp' Tue Jun 30 19:49:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Asp' Q9BZS1-1:p.Ser418Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S C Wed May 02 16:31:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418cys' Tue Jun 30 17:49:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Cys' Q9BZS1-1:p.Ser418Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:00:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418ala' Tue Jun 30 20:20:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Ala' Q9BZS1-1:p.Ser418Gly mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S G Wed May 02 16:46:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418gly' Tue Jun 30 19:11:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Gly' ser422 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 7 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4A Mutations of Ser422 or Ser418 either increased or decreased detection of Serine phosphorylated at residue 422. foxp3-il2_human_gene-1 3 5 unspecified role unspecified role prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BZS1-1:p.Ser418Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:31:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418asp' Tue Jun 30 17:11:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Asp' Q9BZS1-1:p.Ser422Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:44:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser422asp' Tue Jun 30 19:26:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser422Asp' association association 4 D/E P11309 decreases binding of Q9BZS1 to ENSG00000109471. Ectopic expression of PIM1 reduced the accumulation of wild-type FOXP3, but not the S422A and S418A mutants. foxp3-il2_human_gene-2 1 5 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BZS1-1:p.Ser418Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:08:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser418asp' Tue Jun 30 18:49:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser418Asp' Q9BZS1-1:p.Ser422Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 20:11:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser422asp' Tue Jun 30 18:36:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser422Asp' association association 7 A CHEBI:86453 increases binding of Q9BZS1-1 to ENSG00000109471. CHEBI:86453 - PIM1i - a specific inhibitor of PIM1. P11309 increases expression at RNA level of ENSG00000109471. the P11309 causality statement from fig 5A - based on fact KO of PIM1 causes loss of IL2 mRNA expression, so presence of PIM1 must increase expression.