IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FRA2 is a STAT5 target gene regulated by IL-2 in human CD4 T cells. FRA2 is a STAT5 target gene regulated by IL-2 in human CD4 T cells. PLoS ONE 2014 imex curation Rani A., Greenlaw R., Runglall M., Jurcevic S., John S. IBD - Inflammatory bowel disease stipo.jurcevic@kcl.ac.uk,susan.john@kcl.ac.uk 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-t-lymphoblast Homo sapiens T-lymphoblast t-lymphoblast T-lymphoblast ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Rani A., Greenlaw R., Runglall M., Jurcevic S., John S. IBD - Inflammatory bowel disease T-lymphoblasts were generated by activating freshly isolated CD4+ T cells using phytohemagglutinin (PHA) (2 micrograms/ml for 72 hours). stipo.jurcevic@kcl.ac.uk,susan.john@kcl.ac.uk Accepted 2015-JAN-29 by ORCHARD PLoS ONE 2014 Only protein-protein interactions imex curation fosl2_human_gene Fos-related antigen 2 (FOSL2) human gene gene gene human Homo sapiens Human sta5a_human Signal transducer and activator of transcription 5A STAT5A STAT5 protein protein human Homo sapiens Human MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS DIP protein P42229 original sequence version: 162 C64237295F88CFBE sta5b_human Signal transducer and activator of transcription 5B STAT5B protein protein human Homo sapiens Human MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS AA2F1CAB20955ACA stat5a-fosl2-1 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey promoter region gas2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined promoter region gas1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4c human interleukin-2 (IL-2) (P60568) (Teceleukin/TECIN) More GAS1 (intronic site)-containing DNA co-precipitated with anti-STAT5A antibody compared to GAS2 (upstream site)-containing DNA. P42229 increases expression at RNA level of ENSG00000075426 P60568 increases binding of P42229 to ENSG00000075426 P60568 indirectly increases expression at RNA level of ENSG00000075426 stat5b-fosl2-1 1 2 unspecified role unspecified role prey prey promoter region gas1 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined promoter region gas2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait association association Fig. 4c More GAS1 (intronic site)-containing DNA co-precipitated with anti-STAT5B antibody compared to GAS2 (upstream site)-containing DNA. For ChIP with anti-STAT5B antibody, only results for IL-2-treated T-lymphoblasts are shown, not for untreated control cells, hence IL-2 was not added as an agonist here. P51692 increases expression at RNA level of ENSG00000075426