IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phosphorylation of Carma1, but not Bcl10, by Akt regulates TCR/CD28-mediated NF-κB induction and cytokine production. Phosphorylation of Carma1, but not Bcl10, by Akt regulates TCR/CD28-mediated NF-κB induction and cytokine production. Mol. Immunol. (0161-5890) 2014 imex curation Cheng J., Hamilton K.S., Kane L.P. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The authors mention Akt autophosphorylation in Fig. 3b/c, but I didn't add this interaction yet as I don't really understand why the autophosphorylated Akt is not detected in lanes with Carma1 mutants? lkane@pitt.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Cheng J., Hamilton K.S., Kane L.P. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis This experiment is referred to in the text and figure 3 legend as being shown in Fig. 3a and 3b, while actual figure numbering is 3b and 3c, respectively. The authors mention Akt autophosphorylation in Fig. 3b/c, but I didn't add this interaction yet as I don't really understand why the autophosphorylated Akt is not detected in lanes with Carma1 mutants? Information about active recombinant human Akt1 proteins were obtained from the supplier's website (Millipore). lkane@pitt.edu Accepted 2015-JAN-21 by ORCHARD Mol. Immunol. (0161-5890) 2014 Only protein-protein interactions imex curation car11_human Caspase recruitment domain-containing protein 11 CARD11 CARMA1 CARD-containing MAGUK protein 1 protein protein human Homo sapiens Human MPGGGPEMDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPKKEQVLELERENEMLKTKNQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEESPEDSKYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNSFTKMQPPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHERYSFGPSSIHSSSSSHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQHTTLNGDSLTSQLTLLGGNARGSFVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVPLDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYRKLVKDMEDGLITSGDSFYIRLNLNISSQLDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLDMGTIPSYSRAQQLLLVKLQRLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLVRAFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSREKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL DIP protein Q9BXL7 original sequence version: 69 DIP protein Q9BXL7 original sequence version: 87 DIP protein Q9BXL7 original sequence version: 85 DIP protein Q9BXL7 original sequence version: 94 mint homomint Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10 2F3512D903795D18 akt1_human RAC-alpha serine/threonine-protein kinase AKT1 PKB RAC RAC-PK-alpha Protein kinase B Proto-oncogene c-Akt Protein kinase B alpha protein protein human Homo sapiens Human MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA DIP protein P31749 original sequence version: 184 DIP protein P31749 original sequence version: 168 DIP protein P31749 original sequence version: 102 DIP protein P31749 original sequence version: 110 DIP protein P31749 original sequence version: 160 DIP protein P31749 original sequence version: 136 DIP protein P31749 original sequence version: 175 DIP protein P31749 original sequence version: 201 DIP protein P31749 original sequence version: 166 DIP protein P31749 original sequence version: 178 DIP protein P31749-1 original sequence version: 194 DIP protein P31749 original sequence version: 106 DIP protein P31749 original sequence version: 141 6EAFF4F8AD436714 akt1-card11 1 2 enzyme target enzyme target substrate substrate neutral component neutral component Q9BXL7:p.Ser644Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:52:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser644ala' Tue Jun 30 18:44:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser644Ala' n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BXL7:p.[Ser644Ala;Ser652Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Fri Jul 22 11:04:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser652ala,ser644ala' Wed May 02 16:28:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser644ala,ser652ala' Tue Jun 30 16:59:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser644Ala;Ser652Ala]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BXL7:p.Ser652Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:53:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser652ala' Tue Jun 30 18:02:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser652Ala' Q9BXL7:p.Ser558_Ser559delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain SS AA Thu Jul 07 12:17:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ser558ala_ser559ala Fri Jul 22 11:12:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser558ala,ser559ala' Wed May 02 15:29:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_ser558-559ala_ala' Tue Jun 30 18:35:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser558_Ser559delinsAlaAla' ecobb Escherichia coli (strain B / BL21) 3 enzyme enzyme neutral component neutral component ser/thr_dual-sp_kinase sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 13 9 phosphorylation phosphorylation reaction phosphorylation Fig. 3b, 3c P31749 is required for phosphorylation of Q9BXL7 P31749 increases activity of Q9BXL7 Q9BXL7 ser-558 O-phospho-L-serine Q9BXL7 ser-644 O-phospho-L-serine Q9BXL7 ser-652 O-phospho-L-serine