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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
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        <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>The guggulsterone derivative GG-52 inhibits NF-κB signaling in bone marrow-derived dendritic cells and attenuates colitis in IL-10 knockout mice.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="23603141" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10899378" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-24370" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">The guggulsterone derivative GG-52 inhibits NF-κB signaling in bone marrow-derived dendritic cells and attenuates colitis in IL-10 knockout mice.</attribute>
            <attribute name="journal" nameAc="MI:0885">Life sciences</attribute>
            <attribute name="publication year" nameAc="MI:0886">2013</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Kang SJ., Kim JM., Koh SJ., Kim SH., Im JP., Jung HC., Kim JS.</attribute>
            <attribute name="dataset" nameAc="MI:0875">IBD - Inflammatory bowel disease</attribute>
            <attribute name="remark-internal">sorry - I had not realized there was so little in this publication before I got into doing it</attribute>
            <attribute name="contact-email" nameAc="MI:0634">jooskim@snu.ac.kr</attribute>
            <attribute name="author-announcement">17-Sep-2015: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="23603141" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-24370" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>emsa supershift</shortLabel>
            <fullName>electrophoretic mobility supershift assay</fullName>
            <alias type="synonym" typeAc="MI:1041">emsa supershift</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0412" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-705588" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="12169687" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>weight autoradiogra</shortLabel>
            <fullName>molecular weight estimation by autoradiography</fullName>
            <alias type="synonym" typeAc="MI:1041">weight autoradiogra</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0821" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218638" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Kang SJ., Kim JM., Koh SJ., Kim SH., Im JP., Jung HC., Kim JS.</attribute>
          <attribute name="dataset" nameAc="MI:0875">IBD - Inflammatory bowel disease</attribute>
          <attribute name="remark-internal">sorry - I had not realized there was so little in this publication before I got into doing it</attribute>
          <attribute name="contact-email" nameAc="MI:0634">jooskim@snu.ac.kr</attribute>
          <attribute name="accepted">Accepted 2015-JUL-29 by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">Life sciences</attribute>
          <attribute name="publication year" nameAc="MI:0886">2013</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>consensus_nfkb_site_probe</shortLabel>
          <fullName>NFKB CONSENSUS OLIGONUCLEOTIDE</fullName>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-10918357" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>ss dna</shortLabel>
            <fullName>single stranded deoxyribonucleic acid</fullName>
            <alias type="go synonym" typeAc="MI:0303">ss DNA</alias>
            <alias type="synonym" typeAc="MI:1041">ss dna</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0680" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-967913" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="-2">
          <names>
            <shortLabel>chemical synthesis</shortLabel>
            <fullName>Chemical synthesis (Chemical synthesis)</fullName>
          </names>
        </organism>
        <sequence>AGTTGAGGGGACTTTCCCAGGC</sequence>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>p25799-pro_0000030313</shortLabel>
          <fullName>Nuclear factor NF-kappa-B p50 subunit</fullName>
          <alias type="gene name" typeAc="MI:0301">Nfkb1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">DNA-binding factor KBF1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">EBP-1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">NF-kappa-B1 p84/NF-kappa-B1 p98</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P25799-PRO_0000030313" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1209141" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-643958" refType="chain-parent" refTypeAc="MI:0951"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="10090">
          <names>
            <shortLabel>mouse</shortLabel>
            <fullName>Mus musculus</fullName>
            <alias type="synonym" typeAc="MI:1041">Mouse</alias>
          </names>
        </organism>
        <sequence>MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHG</sequence>
        <attributeList>
          <attribute name="crc64">0597F8EA07635A94</attribute>
          <attribute name="chain-seq-start">1</attribute>
          <attribute name="chain-seq-end">431</attribute>
          <attribute name="crc64">0597F8EA07635A94</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-24370-1">
        <names>
          <shortLabel>consensus_nfkb_site_probe-nfkb1-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-10919179" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-24370-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="10090">
                <names>
                  <shortLabel>mouse-bmdc</shortLabel>
                  <fullName>Mus musculus bone marrow-derived dendritic cells</fullName>
                </names>
                <tissue>
                  <names>
                    <shortLabel>bmdm-1</shortLabel>
                    <fullName>bone marrow-derived macrophage</fullName>
                  </names>
                  <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="IA:2411" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-7296588" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="brenda" dbAc="MI:0864" id="BTO:0004732" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-446336" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                </tissue>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="6">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="7">
                <names>
                  <shortLabel>N/A</shortLabel>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-10919182" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>32p radiolabel</shortLabel>
                    <fullName>32p radiolabel</fullName>
                    <alias type="go synonym" typeAc="MI:0303">P32</alias>
                    <alias type="go synonym" typeAc="MI:0303">32P</alias>
                    <alias type="synonym" typeAc="MI:1041">32P</alias>
                    <alias type="synonym" typeAc="MI:1041">P32</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0236" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608952" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
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                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">2</attribute>
          <attribute name="antagonist" nameAc="MI:0626">GG-52 -a guggulsterone derivative</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
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