UniProt UniProt http://www.uniprot.org Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org Accepted 2016-JAN-29 AT 08:26 GMT AT 08:26 GMT by ORCHARD American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org Accepted 2016-JAN-29 AT 08:26 GMT AT 08:26 GMT by ORCHARD American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org Accepted 2016-JAN-29 AT 08:26 GMT AT 08:26 GMT by ORCHARD American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org Accepted 2016-JAN-29 AT 08:26 GMT AT 08:26 GMT by ORCHARD American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. Direct methylation of FXR by Set7/9, a lysine methyltransferase, regulates the expression of FXR target genes. American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation Balasubramaniyan N., Ananthanarayanan M., Suchy FJ., Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Balasubramaniyan N., Ananthanarayanan M., Suchy FJ. IBD - Inflammatory bowel disease Sequence coordinates for NRH1H4/FXR (binding sites, mutants) adapted to UniProt canonical seq frederick.suchy@childrenscolorado.org Accepted 2016-JAN-29 AT 08:26 GMT AT 08:26 GMT by ORCHARD American journal of physiology. Gastrointestinal and liver physiology 2012 imex curation setd7_human Histone-lysine N-methyltransferase SETD7 SETD7 KIAA1717 SET7 KMT7 SET9 Histone H3-K4 methyltransferase SETD7 SET domain-containing protein 7 SET7/9 Lysine N-methyltransferase 7 protein protein human Homo sapiens Human MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK DIP protein Q8WTS6 original sequence version: 46 DIP protein Q8WTS6 original sequence version: 89 DIP protein Q8WTS6 original sequence version: 106 DIP protein Q8WTS6 original sequence version: 120 DIP protein Q8WTS6 original sequence version: 121 DIP protein Q8WTS6 original sequence version: 114 DIP protein Q8WTS6 original sequence version: 95 73A1217079E3BA13 nr1h4_human Bile acid receptor NR1H4 BAR FXR HRR1 RIP14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 protein protein human Homo sapiens Human MVMQFQGLENPIQISPHCSCTPSGFFMEMMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ C23283576A8CF76B nr1h4-setd7-1 1 6 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q96RI1:p.Lys218Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:03:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys218arg' Tue Jun 30 15:49:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys218Arg' Q96RI1:p.Lys220Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K R Wed May 02 17:42:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys220arg' Tue Jun 30 19:27:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys220Arg' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) methylation methylation reaction methylation 6c nr1h4-setd7-2 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 8a setd7-nr1h4-1 4 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 8b nr1h4-setd7-3 5 6 unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q96RI1:p.Lys220Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 15:59:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys220arg' Tue Jun 30 20:24:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys220Arg' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 8c nr1h4-setd7-4 2 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q96RI1:p.Lys220Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 14:31:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys220arg' Tue Jun 30 16:19:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys220Arg' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 9 GW 4064 (synthetic FXR agonist)