IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Mol. Cell. Biochem. (0300-8177) 2010 imex curation Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn Accepted 2014-OCT-23 by ORCHARD Mol. Cell. Biochem. (0300-8177) 2010 Only protein-protein interactions imex curation Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Mol. Cell. Biochem. (0300-8177) 2010 imex curation Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn Accepted 2014-OCT-20 by ORCHARD Mol. Cell. Biochem. (0300-8177) 2010 Only protein-protein interactions imex curation Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Ubiquitin ligase Smurf1 targets TRAF family proteins for ubiquitination and degradation. Mol. Cell. Biochem. (0300-8177) 2010 imex curation Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions baker's yeast-mav203 Saccharomyces cerevisiae mav203 Saccharomyces cerevisiae 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li S., Lu K., Wang J., An L., Yang G., Chen H., Cui Y., Yin X., Xie P., Xing G., He F., Zhang L. IBD - Inflammatory bowel disease hefc@nic.bmi.ac.cn,zhanglq@nic.bmi.ac.cn Accepted 2014-OCT-20 by ORCHARD human adult brain cDNA library Mol. Cell. Biochem. (0300-8177) 2010 Only protein-protein interactions imex curation traf4_human TNF receptor-associated factor 4 TRAF4 CART1 MLN62 RNF83 Cysteine-rich domain associated with RING and Traf domains protein 1 Metastatic lymph node gene 62 protein RING finger protein 83 protein protein human Homo sapiens Human MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKPGTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS A3F57E0E1081AB88 q9hce7-2 E3 ubiquitin-protein ligase SMURF1 HECT-type E3 ubiquitin transferase SMURF1 SMURF1 KIAA1625 SMAD ubiquitination regulatory factor 1 SMAD-specific E3 ubiquitin-protein ligase 1 protein protein human Homo sapiens Human MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE F106E00CA3C210AA smurf1-traf4 3 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait ww_dom sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 8 physical association physical association Fig. 1a smurf1-traf4-1 1 4 unspecified role unspecified role prey prey math sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9BUZ4:p.[Pro60_Tyr63delinsAlaAlaAlaAla;Pro194_Tyr197delinsAlaAlaAlaAla;Pro335Ala;Phe337_Tyr338delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PLDY AAAA certain certain sequence position certain certain certain sequence position certain FY AA certain certain sequence position certain certain certain sequence position certain PCTY AAAA Thu Jul 07 12:17:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was pro60ala_leu61ala_asp62ala_tyr63ala_pro194ala_cys195ala_thr196ala_tyr197ala_pro335ala_phe337ala_tyr338ala Fri Jul 22 10:56:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro60ala,leu61ala,asp62ala,tyr63ala,pro194ala,cys195ala,thr196ala,tyr197ala,pro335ala,phe337ala,tyr338ala' Wed May 02 19:44:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_leu_asp_tyr60-63ala_ala_ala_ala,pro_cys_thr_tyr194-197ala_ala_ala_ala,pro335ala,phe_tyr337-338ala_ala' Tue Jun 30 18:38:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro60_Tyr63delinsAlaAlaAlaAla;Pro194_Tyr197delinsAlaAlaAlaAla;Pro335Ala;Phe337_Tyr338delinsAlaAla]' znf_ring sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range znf_traf sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1b, 2b, 2e traf4-smurf1 1 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww_dom required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range Interacting construct contains the second WW domain of Smurf1. physical association physical association Fig. 1b, 2a traf4-smurf1-1 2 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Fig. 1c Q9HCE7-2 increases degradation at protein level of Q9BUZ4 Q9HCE7-2 is required for ubiquitination of Q9BUZ4