IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. J. Biol. Chem. (0021-9258) 2009 imex curation Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease ychi@uky.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis ychi@uky.edu Accepted 2014-OCT-28 by ORCHARD J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. J. Biol. Chem. (0021-9258) 2009 imex curation Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease ychi@uky.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis Affinities calculated from the ITC assay are given as association constants (Ka) in table 2. The values given here are dissociation constants (Kd) obtained by transforming the Ka values from table 2 (Kd = 1/Ka). ychi@uky.edu Accepted 2014-OCT-28 by ORCHARD J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. J. Biol. Chem. (0021-9258) 2009 imex curation Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease ychi@uky.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis ychi@uky.edu Accepted 2014-OCT-28 by ORCHARD J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. J. Biol. Chem. (0021-9258) 2009 imex curation Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease ychi@uky.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Rha G.B., Wu G., Shoelson S.E., Chi Y.I. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis ychi@uky.edu Accepted 2014-OCT-28 by ORCHARD J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation prgc1_human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ligand effect modulator 6 PPARGC1A LEM6 PGC1 PGC1A PPARGC1 protein protein human Homo sapiens Human MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR DIP protein Q9UBK2 original sequence version: 127 DIP protein Q9UBK2 original sequence version: 104 97DDD3643051A5F1 pparg_human Peroxisome proliferator-activated receptor gamma Nuclear receptor subfamily 1 group C member 3 PPARG NR1C3 protein protein human Homo sapiens Human MGETLGDSPIDPESDSFTDTLSANISQEMTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY 3933EFF36A0E4CAF hnf4a_human Hepatocyte nuclear factor 4-alpha Transcription factor HNF-4 Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 HNF4A HNF4 NR2A1 TCF14 protein protein human Homo sapiens Human MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI 5F1309B89D95DCAF hnf4a-ppargc1a 4 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9UBK2:p.Leu147_Leu148delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:16:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala Fri Jul 22 11:10:44 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala' Wed May 02 17:33:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala' Tue Jun 30 16:33:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu147_Leu148delinsAlaAla' Q9UBK2:p.Tyr213_Leu214delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:16:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was tyr213ala_leu214ala Fri Jul 22 10:55:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr213ala,leu214ala' Wed May 02 16:05:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_leu213-214ala_ala' Tue Jun 30 18:01:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr213_Leu214delinsAlaAla' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain VL AA Thu Jul 07 12:16:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala Fri Jul 22 11:06:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala' Wed May 02 16:22:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala' Tue Jun 30 18:59:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla]' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain YL AA certain certain sequence position certain certain certain sequence position certain VL AA Thu Jul 07 12:17:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_tyr213ala_leu214ala Fri Jul 22 11:01:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,tyr213ala,leu214ala' Wed May 02 15:03:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 18:35:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Tyr213_Leu214delinsAlaAla]' Q9UBK2:p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:17:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 10:56:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 15:24:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 15:37:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:17:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 11:08:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 16:34:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 15:48:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hormone_rcpt_ligand-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Fig. 5a Q9UBK2 increases activity of P41235 pparg-ppargc1a-2 4 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined nucl_hormone_rcpt_ligand-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9UBK2:p.Leu147_Leu148delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:17:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala Fri Jul 22 11:07:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala' Wed May 02 15:11:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala' Tue Jun 30 18:31:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu147_Leu148delinsAlaAla' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:17:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala Fri Jul 22 10:57:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala' Wed May 02 15:16:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala' Tue Jun 30 18:42:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla]' Q9UBK2:p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:17:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 11:14:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 17:16:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 16:22:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:07:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 10:59:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 18:16:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 18:09:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' in vitro In vitro direct interaction direct interaction Suppl. Fig. S5b Triglitazone (1 micromolar) was added as an agonist for PPAR-gamma. Q9UBK2 increases activity of P37231 Based on the region they used I think they use P37231-2 isoform of PPARgamma, which is referred to as wt; however, this is never explicitly mentioned in the paper so I used the conanical P37231 sequence. hnf4a-ppargc1a-1 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9UBK2:p.Val91_Leu92delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain VL AA Thu Jul 07 12:16:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala Fri Jul 22 11:12:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala' Wed May 02 17:42:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala' Tue Jun 30 18:27:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val91_Leu92delinsAlaAla' Q9UBK2:p.Leu147_Leu148delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:16:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala Fri Jul 22 11:01:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala' Wed May 02 16:54:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala' Tue Jun 30 18:09:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu147_Leu148delinsAlaAla' Q9UBK2:p.Tyr213_Leu214delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:16:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was tyr213ala_leu214ala Fri Jul 22 11:11:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr213ala,leu214ala' Wed May 02 19:03:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_leu213-214ala_ala' Tue Jun 30 16:37:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr213_Leu214delinsAlaAla' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:16:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala Fri Jul 22 10:59:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala' Wed May 02 15:15:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala' Tue Jun 30 16:31:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla]' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain YL AA Thu Jul 07 12:16:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_tyr213ala_leu214ala Fri Jul 22 11:03:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,tyr213ala,leu214ala' Wed May 02 15:04:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 16:54:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Tyr213_Leu214delinsAlaAla]' Q9UBK2:p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain YL AA certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:16:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 10:51:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 15:49:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 19:01:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' Q9UBK2:p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain YL AA certain certain sequence position certain certain certain sequence position certain VL AA certain certain sequence position certain certain certain sequence position certain LL AA Thu Jul 07 12:16:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val91ala_leu92ala_leu147ala_leu148ala_tyr213ala_leu214ala Fri Jul 22 11:14:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val91ala,leu92ala,leu147ala,leu148ala,tyr213ala,leu214ala' Wed May 02 19:49:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_leu91-92ala_ala,leu_leu147-148ala_ala,tyr_leu213-214ala_ala' Tue Jun 30 18:37:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val91_Leu92delinsAlaAla;Leu147_Leu148delinsAlaAla;Tyr213_Leu214delinsAlaAla]' ecobd Escherichia coli (strain B / BL21-DE3) 7 unspecified role unspecified role neutral component neutral component nucl_hormone_rcpt_ligand-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 41 32 direct interaction direct interaction Fig. 5b, Table 2 Temp = 20C, pH = 6 The wild type PGC-1alpha construct (residues 74-219) binds with Kd = 1.23 x 10E-6 molar. Mutations in the different LxxLL motifs decrease binding to: Kd = 1.95 x 10E-6 molar (val91ala_leu92ala); Kd = 1.47 x 10E-5 molar (leu147ala_leu148ala); Kd = 2.32 x 10E-6 molar (tyr213ala_leu214ala); Kd = 1.82 x 10E-5 molar (val91ala_leu92ala_leu147ala_leu148ala); and Kd = 3.00 x 10E-6 molar (val91ala_leu92ala_tyr213ala_leu214ala). hnf4a-ppargc1a-2 3 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 7 unspecified role unspecified role neutral component neutral component nucl_hormone_rcpt_ligand-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 46 44 direct interaction direct interaction Table 1 2.20A working 19.0%, free 23.6% Although the PGC-1alpha construct contains three LxxLL motifs, which mediate binding to the ligand-binding domain (LBD) of nuclear receptors like HNF4alpha, the crystal structure shows only one LxxLL motif bound to HNF4alpha. However, the electron densities for the side chains of the bound motif are poorly defined except for except for the leucine residues that constitute the only common residues between the three motifs in PGC-1alpha. This indicates that the bound motif in the crystal structure is not a single sequence but an averaged structure of more than one LxxLL motif. hnf4a-ppargc1a-3 1 5 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 7 unspecified role unspecified role neutral component neutral component nucl_hormone_rcpt_ligand-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction Suppl. Fig. S1