IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells. Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells. Leukemia (0887-6924) 2008 imex curation Lesniewski M.L., Haviernik P., Weitzel R.P., Kadereit S., Kozik M.M., Fanning L.R., Yang Y.C., Hegerfeldt Y., Finney M.R., Ratajczak M.Z., Greco N., Paul P., Maciejewski J., Laughlin M.J. IBD - Inflammatory bowel disease In Fig. 4b, the ChIP experiment with anti-NFAT1/NFATc2 is performed in both UCB and adult PB CD4+ T cells; however, both lane 3 and 4 are labeled with UCB CD4+, while the text and figure legend state that lane 3 (negative result) uses genomic DNA from UCB T cells and lane 4 (positive result) uses genomic DNA from PB T cells. mary.laughlin@case.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-pb_t-cell_cd4+ Homo sapiens peripheral blood cd4 positive T cell pb_t-cell_cd4+ peripheral blood cd4 positive T cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lesniewski M.L., Haviernik P., Weitzel R.P., Kadereit S., Kozik M.M., Fanning L.R., Yang Y.C., Hegerfeldt Y., Finney M.R., Ratajczak M.Z., Greco N., Paul P., Maciejewski J., Laughlin M.J. IBD - Inflammatory bowel disease In Fig. 4b, the ChIP experiment with anti-NFAT1/NFATc2 is performed in both UCB and adult PB CD4+ T cells; however, both lane 3 and 4 are labeled with UCB CD4+, while the text and figure legend state that lane 3 (negative result) uses genomic DNA from UCB T cells and lane 4 (positive result) uses genomic DNA from PB T cells. mary.laughlin@case.edu Accepted 2015-JAN-29 by ORCHARD Leukemia (0887-6924) 2008 Only protein-protein interactions imex curation Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells. Regulation of IL-2 expression by transcription factor BACH2 in umbilical cord blood CD4+ T cells. Leukemia (0887-6924) 2008 imex curation Lesniewski M.L., Haviernik P., Weitzel R.P., Kadereit S., Kozik M.M., Fanning L.R., Yang Y.C., Hegerfeldt Y., Finney M.R., Ratajczak M.Z., Greco N., Paul P., Maciejewski J., Laughlin M.J. IBD - Inflammatory bowel disease In Fig. 4b, the ChIP experiment with anti-NFAT1/NFATc2 is performed in both UCB and adult PB CD4+ T cells; however, both lane 3 and 4 are labeled with UCB CD4+, while the text and figure legend state that lane 3 (negative result) uses genomic DNA from UCB T cells and lane 4 (positive result) uses genomic DNA from PB T cells. mary.laughlin@case.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-ucb_t-cell_cd4+ Homo sapiens umbilical cord blood cd4 positive T cell ucb_t-cell_cd4+ umbilical cord blood cd4 positive T cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lesniewski M.L., Haviernik P., Weitzel R.P., Kadereit S., Kozik M.M., Fanning L.R., Yang Y.C., Hegerfeldt Y., Finney M.R., Ratajczak M.Z., Greco N., Paul P., Maciejewski J., Laughlin M.J. IBD - Inflammatory bowel disease In Fig. 4b, the ChIP experiment with anti-NFAT1/NFATc2 is performed in both UCB and adult PB CD4+ T cells; however, both lane 3 and 4 are labeled with UCB CD4+, while the text and figure legend state that lane 3 (negative result) uses genomic DNA from UCB T cells and lane 4 (positive result) uses genomic DNA from PB T cells. mary.laughlin@case.edu Accepted 2015-JAN-29 by ORCHARD Leukemia (0887-6924) 2008 Only protein-protein interactions imex curation bach2_human Transcription regulator protein BACH2 BACH2 BTB and CNC homolog 2 protein protein human Homo sapiens Human MSVDEKPDSPMYVYESTVHCTNILLGLNDQRKKDILCDVTLIVERKEFRAHRAVLAACSEYFWQALVGQTKNDLVVSLPEEVTARGFGPLLQFAYTAKLLLSRENIREVIRCAEFLRMHNLEDSCFSFLQTQLLNSEDGLFVCRKDAACQRPHEDCENSAGEEEDEEEETMDSETAKMACPRDQMLPEPISFEAAAIPVAEKEEALLPEPDVPTDTKESSEKDALTQYPRYKKYQLACTKNVYNASSHSTSGFASTFREDNSSNSLKPGLARGQIKSEPPSEENEEESITLCLSGDEPDAKDRAGDVEMDRKQPSPAPTPTAPAGAACLERSRSVASPSCLRSLFSITKSVELSGLPSTSQQHFARSPACPFDKGITQGDLKTDYTPFTGNYGQPHVGQKEVSNFTMGSPLRGPGLEALCKQEGELDRRSVIFSSSACDQVSTSVHSYSGVSSLDKDLSEPVPKGLWVGAGQSLPSSQAYSHGGLMADHLPGRMRPNTSCPVPIKVCPRSPPLETRTRTSSSCSSYSYAEDGSGGSPCSLPLCEFSSSPCSQGARFLATEHQEPGLMGDGMYNQVRPQIKCEQSYGTNSSDESGSFSEADSESCPVQDRGQEVKLPFPVDQITDLPRNDFQMMIKMHKLTSEQLEFIHDVRRRSKNRIAAQRCRKRKLDCIQNLECEIRKLVCEKEKLLSERNQLKACMGELLDNFSCLSQEVCRDIQSPEQIQALHRYCPVLRPMDLPTASSINPAPLGAEQNIAASQCAVGENVPCCLEPGAAPPGPPWAPSNTSENCTSGRRLEGTDPGTFSERGPPLEPRSQTVTVDFCQEMTDKCTTDEQPRKDYT 4E926AC325952A93 junb_human Transcription factor jun-B JUNB protein protein human Homo sapiens Human MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGGVTEEQEGFADGFVKALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGAAVGTGSSYPTTTISYLPHAPPFAGGHPAQLGLGRGASTFKEEPQTVPEARSRDATPPVSPINMEDQERIKVERKRLRNRLAATKCRKRKLERIARLEDKVKTLKAENAGLSSTAGLLREQVAQLKQKVMTHVSNGCQLLLGVKGHAF DIP protein P17275 original sequence version: 151 DIP protein P17275 original sequence version: 118 DIP protein P17275 original sequence version: 143 DF8CD1CD4409C6BE nfac2_human Nuclear factor of activated T-cells, cytoplasmic 2 T-cell transcription factor NFAT1 NFAT pre-existing subunit NFATC2 NFAT1 NFATP protein protein human Homo sapiens Human MNAPERQPQPDGGDAPGHEPGGSPQDELDFSILFDYEYLNPNEEEPNAHKVASPPSGPAYPDDVLDYGLKPYSPLASLSGEPPGRFGEPDRVGPQKFLSAAKPAGASGLSPRIEITPSHELIQAVGPLRMRDAGLLVEQPPLAGVAASPRFTLPVPGFEGYREPLCLSPASSGSSASFISDTFSPYTSPCVSPNNGGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVALPPGASPQRSRSPSPQPSSHVAPQDHGSPAGYPPVAGSAVIMDALNSLATDSPCGIPPKMWKTSPDPSPVSAAPSKAGLPRHIYPAVEFLGPCEQGERRNSAPESILLVPPTWPKPLVPAIPICSIPVTASLPPLEWPLSSQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIECSQRSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKVVFTEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKHIRTPVKVNFYVINGKRKRSQPQHFTYHPVPAIKTEPTDEYDPTLICSPTHGGLGSQPYYPQHPMVAESPSCLVATMAPCQQFRTGLSSPDARYQQQNPAAVLYQRSKSLSPSLLGYQQPALMAAPLSLADAHRSVLVHAGSQGQSSALLHPSPTNQQASPVIHYSPTNQQLRCGSHQEFQHIMYCENFAPGTTRPGPPPVSQGQRLSPGSYPTVIQQQNATSQRAAKNGPPVSDQKEVLPAGVTIKQEQNLDQTYLDDVNEIIRKEFSGPPARNQT DIP protein Q13469 original sequence version: 99 DIP protein Q13469 original sequence version: 100 DIP protein Q13469 original sequence version: 167 8DAE86855CCB58D3 il2_human_gene Human IL-2 gene IL2 gene gene human Homo sapiens Human nfatc2-il2-1 1 6 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait association association Fig. 4b IL-2 promoter DNA co-precipitated with anti-NFAT1/NFATc2 antibody in CD4+ T cells from adult peripheral blood but not from umbilical cord blood. bach2-il2-1 2 6 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait association association Fig. 4a, 4b IL-2 promoter DNA co-precipitated with anti-BACH2 antibody in CD4+ T cells from umbilical cord blood but not from adult peripheral blood. Q9BYV9 increases expression at RNA level of ENSG00000109471 Q9BYV9 increases expression at protein level of ENSG00000109471 junb-il2-1 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4b