IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hepatocytes Human-hepatocytes primary hepatocytes primary hepatocytes ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hepatocytes Human-hepatocytes primary hepatocytes primary hepatocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis ChIP signals for proteins that bind indirectly to DNA were improved by incubating cells with the homobifunctional, cleavable cross-linking reagent DTBP (dimethyl-3,3-dithiobispropionimidate-2HCl) on ice for 30 minutes. gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Dexamethasone-mediated up-regulation of human CYP2A6 involves the glucocorticoid receptor and increased binding of hepatic nuclear factor 4 alpha to the proximal promoter. Mol. Pharmacol. (0026-895X) 2008 imex curation Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease gkirby@uoguelph.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Onica T., Nichols K., Larin M., Ng L., Maslen A., Dvorak Z., Pascussi J.M., Vilarem M.J., Maurel P., Kirby G.M. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis gkirby@uoguelph.ca Accepted 2014-NOV-12 by ORCHARD Mol. Pharmacol. (0026-895X) 2008 Only protein-protein interactions imex curation gcr_human Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 NR3C1 GRL protein protein human Homo sapiens Human MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRPDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK C5C90C9A5DD16AAB h4_human Histone H4 H4-16 H4C1 H4C11 H4C12 H4C13 H4C14 H4C15 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A HIST2H4B HIST4H4 protein protein human Homo sapiens Human MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG DIP protein P62805 original sequence version: 68 DIP protein P62805 original sequence version: 111 DIP protein P62805 original sequence version: 94 DIP protein P62805 original sequence version: 97 DIP protein P62805 original sequence version: 116 DIP protein P62805 original sequence version: 105 DIP protein P62805 original sequence version: 120 DIP protein P62805 original sequence version: 144 DIP protein P62805 original sequence version: 150 DIP protein P62805 original sequence version: 154 DIP protein P62805 original sequence version: 156 DIP protein P62805 original sequence version: 112 DIP protein P62805 original sequence version: 121 DIP protein P62805 original sequence version: 123 DIP protein P62805 original sequence version: 126 DIP protein P62805 original sequence version: 132 DIP protein P62805 original sequence version: 136 DIP protein P62805 original sequence version: 119 A9E5DFD3F8B97598 hnf4a_human Hepatocyte nuclear factor 4-alpha Transcription factor HNF-4 Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 HNF4A HNF4 NR2A1 TCF14 protein protein human Homo sapiens Human MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI 5F1309B89D95DCAF cp2a6_human_gene Cytochrome P450 2A6 (CP2A6) human gene gene gene human Homo sapiens Human cp2a6_human_hnf4a-site_dna-probe Human CP2A6 gene promoter HNF4a response element DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human GTTGGGAGGTGAAATGAGGTAAT nr3c1-hnf4a 2 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 5b dexamethasone (CHEBI:41879), a synthetic glucocorticoid nr3c1-hnf4a-1 3 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 5b cp2a6-hnf4a 6 9 unspecified role unspecified role prey prey human-hepatocytes Human-hepatocytes primary hepatocytes primary hepatocytes 11 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 6a cp2a6-hnf4a-1 4 7 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 9 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 11 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) association association Fig. 6b dexamethasone (CHEBI:41879), a synthetic glucocorticoid Two isoforms of glucocorticoid receptor (GR) were detected by the anti-GR antibody, one around 90 kilodaltons (called beta) and one around 94 kilodaltons (called alpha). hnf4a-cp2a6 1 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 7a dexamethasone (CHEBI:41879), a synthetic glucocorticoid No CP2A6 promoter DNA was co-precipitated with anti-HNF4alpha antibody from cells not treated with the synthetic glucocorticoid dexamethasone (CHEBI:41879). P41235 increases expression at RNA level of ENSG00000255974 CHEBI:41879 increases binding of P41235 to ENSG00000255974 hist1h4a-cp2a6 1 8 unspecified role unspecified role bait bait possible acetyllys naclys N-acetylated L-lysine NAcLys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 7a dexamethasone (CHEBI:41879), a synthetic glucocorticoid No CP2A6 promoter DNA was co-precipitated with anti-acetyl-H4 antibody from cells not treated with the synthetic glucocorticoid dexamethasone (CHEBI:41879). CHEBI:41879 increases binding of P62805 to ENSG00000255974 nr3c1-cp2a6 5 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 7b dexamethasone (CHEBI:41879), a synthetic glucocorticoid No CP2A6 promoter DNA was co-precipitated with anti-GR antibody from cells not treated with the synthetic glucocorticoid dexamethasone (CHEBI:41879). CHEBI:41879 increases binding of P04150 to ENSG00000255974