IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation. Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation. The Journal of biological chemistry 2006 imex curation Chen C., Rowell EA., Thomas RM., Hancock WW., Wells AD. IBD - Inflammatory bowel disease adwells@mail.med.upenn.edu Results using anti-AcH3 have not been included due to lack of antibody specificity. 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Chen C., Rowell EA., Thomas RM., Hancock WW., Wells AD. IBD - Inflammatory bowel disease Minimal binding to the IL2/IFNgamma regulatory elements was observed in the absence of TCR stimulation (by anti-CD3/28 or "diacylglycerol analog and Ca2+ ionophore" ). cells were also stimulated with "diacylglycerol analog and Ca2+ ionophore" - but it is not specified if this means PMA +ionomycin. Cd4+ t cells transfected either with FOXP3 or stimulated to express FOXP3. adwells@mail.med.upenn.edu Results using anti-AcH3 have not been included due to lack of antibody specificity. Accepted 2015-OCT-01 AT 11:21 BST AT 11:21 BST by ORCHARD The Journal of biological chemistry 2006 Only protein-protein interactions imex curation imex curation Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation. Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation. The Journal of biological chemistry 2006 imex curation Chen C., Rowell EA., Thomas RM., Hancock WW., Wells AD. IBD - Inflammatory bowel disease adwells@mail.med.upenn.edu Results using anti-AcH3 have not been included due to lack of antibody specificity. 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-jurkat Homo sapiens jurkat Human T cell leukemia ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Chen C., Rowell EA., Thomas RM., Hancock WW., Wells AD. IBD - Inflammatory bowel disease adwells@mail.med.upenn.edu Results using anti-AcH3 have not been included due to lack of antibody specificity. Accepted 2015-OCT-01 AT 11:21 BST AT 11:21 BST by ORCHARD The Journal of biological chemistry 2006 Only protein-protein interactions imex curation imex curation foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP DIP protein Q99JB6 original sequence version: 120 DIP protein Q99JB6 original sequence version: 110 DIP protein Q99JB6 original sequence version: 114 DIP protein Q99JB6 original sequence version: 102 DIP protein Q99JB6 original sequence version: 100 28D5B8E67891840C il2_mouse_gene Interleukin-2 Il2 Il-2 gene gene mouse Mus musculus Mouse ifng_human_gene Interferon gamma IFNG gene gene human Homo sapiens Human il2_human_gene Human IL-2 gene IL2 gene gene human Homo sapiens Human ifng_mouse_gene Interferon gamma Ifng gene gene mouse Mus musculus Mouse foxp3-il2-4 1 4 unspecified role unspecified role prey prey regulatory_regions sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3 C/H/K/L CHEBI:4031 - Cyclsporin A CHEBI:4031 decreases the binding of Q99JB6 to ENSMUSG00000027720 Q99JB6 decreases expression at RNA level of ENSMUSG00000027720 Q99JB6 decreases expression at protein level of ENSMUSG00000027720 causality from Fig 2 E/F foxp3-ifng-1 1 7 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined intronic_enhancer_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3 D/I/J/M CHEBI:4031 decreases the binding of Q99JB6 to ENSMUSG00000055170 CHEBI:4031 -Cyclosporin A The inhibitory effects of Cyclosporin A are only shown for the IFNg intronic enhancer region. Q99JB6 decreases expression at RNA level of ENSMUSG00000055170 Q99JB6 decreases expression at protein level of ENSMUSG00000055170 causality= 2H foxp3-il2-5 1 4 unspecified role unspecified role prey prey regulatory_regions sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait association association Supp 1 foxp3-ifng-2 2 5 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined intronic_enhancer_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3G cells were stimulated by anti-CD28 plus "P/I" which may be PMA plus Ionomycin. Q99JB6 decreases expression at protein level of ENSG00000111537 Q99JB6 decreases expression at RNA level of ENSG00000111537 causality = 2G foxp3-il2-6 2 6 unspecified role unspecified role prey prey regulatory_regions sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 3 E/F cells were stimulated by anti-CD28 plus "P/I" which may be PMA plus Ionomycin.