IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. J. Biol. Chem. (0021-9258) 2006 imex curation Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp Accepted 2015-JAN-16 by ORCHARD J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. J. Biol. Chem. (0021-9258) 2006 imex curation Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp Accepted 2015-JAN-16 by ORCHARD J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. J. Biol. Chem. (0021-9258) 2006 imex curation Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-molt-4 Homo sapiens MOLT-4 T-cell leukaemia line molt-4 Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yoshida K., Liu H., Miki Y. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The PKC delta constructs are described in PMID:9774685 and PMID:11723252. Expression and purification of constructs is based on other papers from these authors and their department (e.g. PMID:17349958, PMID:18307534 and PMID:19911008). Information about recombinant active PKC delta was retrieved from the supplier's website (Calbiochem). yos.mgen@mri.tmd.ac.jp Accepted 2015-JAN-16 by ORCHARD J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation kpcd_human Protein kinase C delta type nPKC-delta PRKCD Tyrosine-protein kinase PRKCD PKCD protein protein human Homo sapiens Human MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED DIP protein Q05655 original sequence version: 92 DIP protein Q05655 original sequence version: 171 013F4314A2EE331A p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 t53i1_human Tumor protein p53-inducible nuclear protein 1 TP53INP1 P53DINP1 SIP Stress-induced protein p53-dependent damage-inducible nuclear protein 1 protein protein human Homo sapiens Human MFQRLNKMFVGEVSSSSNQEPEFNEKEDDEWILVDFIDTCTGFSAEEEEEEEDISEESPTEHPSVFSCLPASLECLADTSDSCFLQFESCPMEESWFITPPPCFTAGGLTTIKVETSPMENLLIEHPSMSVYAVHNSCPGLSEATRGTDELHSPSSPRVEAQNEMGQHIHCYVAALAAHTTFLEQPKSFRPSQWIKEHSERQPLNRNSLRRQNLTRDCHPRQVKHNGWVVHQPCPRQYNY 1950D66EDDB4A186 prkcd-tp53 2 5 enzyme target enzyme target substrate substrate neutral component neutral component n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P04637:p.Ser46Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:51:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser46ala' Tue Jun 30 16:18:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser46Ala' ecolx Escherichia coli 4 enzyme enzyme neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation Fig. 2d P04637 ser-46 O-phospho-L-serine Q05655 is required for phosphorylation of P04637 CHEBI:28748 increases binding of Q05655 to P04637 prkcd-tp53-1 3 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 3a adriamycin (ADR) (CHEBI:28748), a DNA-damaging agent tp53-prkcd 3 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey ser/thr_dual-sp_kinase sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3b adriamycin (ADR) (CHEBI:28748), a DNA-damaging agent prkcd-tp53-2 1 5 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ser/thr_dual-sp_kinase required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range ecolx Escherichia coli physical association physical association Fig. 3c tp53inp1-prkcd 3 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig. 4b adriamycin (ADR) (CHEBI:28748), a DNA-damaging agent The authors also showed co-immunoprecipitation of GFP-tagged p53DINP1 with full-length FLAG-tagged PKC delta using anti-FLAG antibody after transfection of 293T cells with GFP-p53DINP1 and FLAG-PKC delta constructs; however, the origin of the GFP-p53DINP1 construct could not be established, hence this interaction was not captured.