IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Repression of endogenous Smad7 by Ski. Repression of endogenous Smad7 by Ski. J. Biol. Chem. (0021-9258) 2004 imex curation Denissova N.G., Liu F. IBD - Inflammatory bowel disease The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Denissova N.G., Liu F. IBD - Inflammatory bowel disease A derived cell line called SW480.7, which does not express Smad4, was used. The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu Accepted 2015-JAN-29 by ORCHARD J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation Repression of endogenous Smad7 by Ski. Repression of endogenous Smad7 by Ski. J. Biol. Chem. (0021-9258) 2004 imex curation Denissova N.G., Liu F. IBD - Inflammatory bowel disease The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Denissova N.G., Liu F. IBD - Inflammatory bowel disease The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu Accepted 2015-JAN-29 by ORCHARD J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation Repression of endogenous Smad7 by Ski. Repression of endogenous Smad7 by Ski. J. Biol. Chem. (0021-9258) 2004 imex curation Denissova N.G., Liu F. IBD - Inflammatory bowel disease The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Denissova N.G., Liu F. IBD - Inflammatory bowel disease The authors also show co-precipitation of Smad7 promoter DNA (SBE region) with anti-Smad2/3 antibody from HepG2 cells treated with TGF-beta; however, due to the broad specificity of the antibody, the exact identity of the Smad protein participating in this interaction cannot be established, hence this interaction was not captured here. The origin of the constructs used for overexpression of Smad4, Ski, SnoN and TGIF was confirmed to be human by the corresponding author via e-mail. fangliu@cabm.rutgers.edu Accepted 2015-JAN-29 by ORCHARD J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation smad4_human Mothers against decapentaplegic homolog 4 SMAD4 DPC4 MADH4 SMAD family member 4 Deletion target in pancreatic carcinoma 4 protein protein human Homo sapiens Human MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD DIP protein Q13485 original sequence version: 149 DIP protein Q13485 original sequence version: 177 7EE3C4647712DA90 ski_human Ski oncogene SKI Proto-oncogene c-Ski protein protein human Homo sapiens Human MEAAAGGRGCFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAATEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYGNKYKRRVPRVSSEPPASIRPKTDDTSSQSPAPSEKDKPSSWLRTLAGSSNKSLGCVHPRQRLSAFRPWSPAVSASEKELSPHLPALIRDSFYSYKSFETAVAPNVALAPPAQQKVVSSPPCAAAVSRAPEPLATCTQPRKRKLTVDTPGAPETLAPVAAPEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLGSPGARALPSAVPDAAAPADAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQARVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRARPEAAGSEGAAELEP DIP protein P12755 original sequence version: 126 DIP protein P12755 original sequence version: 130 9B78C4840A28C2DA smad7_human_gene Mothers against decapentaplegic homolog 7 SMAD7 MADH7 MADH8 Mothers against decapentaplegic homolog 8 SMAD family member 7 gene gene human Homo sapiens Human smad4-smad7-3 3 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey promoter region smad-binding element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3a, 3b transforming growth factor-beta (TGF-beta) ski-smad7-4 3 6 unspecified role unspecified role prey prey promoter region smad-binding element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait association association Fig. 3a, 3b transforming growth factor-beta (TGF-beta) P12755 decreases expression at RNA level of ENSG00000101665 ski-smad7-1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey promoter region smad-binding element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3e P12755 decreases expression at RNA level of ENSG00000101665 smad4-smad7-1 1 6 unspecified role unspecified role prey prey promoter region smad-binding element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait nucl infection nucleic acid delivery by infection nucl infection association association Fig. 3c Q13485 decreases expression at RNA level of ENSG00000101665 ski-smad7-2 1 6 unspecified role unspecified role prey prey promoter region smad-binding element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait association association Fig. 3c human Smad4 (Q13485) This interaction was only observed in SW480.7 cells in which a retroviral Smad4 expression vector was introduced. Q13485 increases binding of P12755 to ENSG00000101665