UniProt UniProt http://www.uniprot.org In vitro farnesoid X receptor ligand sensor assay using surface plasmon resonance and based on ligand-induced coactivator association. In vitro farnesoid X receptor ligand sensor assay using surface plasmon resonance and based on ligand-induced coactivator association. The Journal of steroid biochemistry and molecular biology 2003 imex curation Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T., Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T. IBD - Inflammatory bowel disease mogami@nihs.go.jp 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T., Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T. IBD - Inflammatory bowel disease FXR ligand binding domain has been preincubated with or without different ligands (CDCA, TDCA, GDCA, CDC-sul, UDCA CA and LCA) mogami@nihs.go.jp Accepted 2016-FEB-08 AT 13:52 GMT AT 13:52 GMT by MGT The Journal of steroid biochemistry and molecular biology 2003 Only protein-protein interactions imex curation imex curation CHEBI:90999 - sodium 3α,7α-dihydroxy-5β-cholane-24-sulfonate CDC-sul submitted chenodeoxycholic acid chenodeoxycholic acid 3alpha,7alpha-Dihydroxy-5beta-cholanic acid 7alpha-hydroxylithocholic acid anthropodeoxycholic acid anthropodesoxycholic acid CDCA chenic acid Chenix Chenodeoxycholic acid Chenodiol gallodesoxycholic acid 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa1_human Nuclear receptor coactivator 1 Steroid receptor coactivator 1 RIP160 Protein Hin-2 Renal carcinoma antigen NY-REN-52 NCOA1 SRC1 BHLHE74 Class E basic helix-loop-helix protein 74 protein protein human Homo sapiens Human MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE DIP protein Q15788-1 original sequence version: 60 DIP protein Q15788-1 original sequence version: 62 DIP protein Q15788 original sequence version: 88 DIP protein Q15788 original sequence version: 107 DIP protein Q15788 original sequence version: 96 25EF6F389489121E taurochenodeoxycholic acid taurochenodeoxycholic acid Chenodeoxycholoyltaurine taurine chenodeoxycholate taurochenodeoxycholate taurochenodeoxycholic acid Taurochenodeoxycholic acid 2-[(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]ethanesulfonic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate CDC-SUL sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glycochenodeoxycholic acid glycochenodeoxycholic acid chenodeoxycholylglycine Chenodeoxyglycocholate chenodeoxyglycocholic acid GCDCA glycine chenodeoxycholate Glycochenodeoxycholate GLYCOCHENODEOXYCHOLIC ACID Glycochenodeoxycholic acid [(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]acetic acid N-(3alpha,7alpha-dihydroxy-5beta-cholan-24-oyl)glycine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q96ri1-2 Bile acid receptor NR1H4 BAR FXR Farnesoid X-activated receptor Farnesol receptor HRR-1 HRR1 Nuclear receptor subfamily 1 group H member 4 RIP14 Retinoid X receptor-interacting protein 14 FXRalpha1(-) FXRalpha2 protein protein human Homo sapiens Human MGSKMNLIEHSHLPTTDEFSFSENLFGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ Produced by alternative splicing of isoform 3. DIP protein Q96RI1-2 original sequence version: 75 DIP protein Q96RI1-2 original sequence version: 109 0A53D414B9049C4C ncoa1-nr1h4-2 1 7 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 2 stimulator stimulator unspecified role unspecified role 3 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:15:41 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'glu685ala' region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q15788:p.[Leu690Ala;Leu693Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:00:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu690ala,leu693ala' Tue Jun 30 19:30:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu690Ala;Leu693Ala]' c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction 1, 2, Table 1 kd without CDCA 36.2 x 10-6 molar CHEBI:16755 is required for the binding of Q15788 to Q96RI1-2 CHEBI:16755 - chenodeoxycholic acid ncoa1-nr1h4-3 1 7 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 stimulator stimulator unspecified role unspecified role 3 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:15:40 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'glu685ala' direct interaction direct interaction Table 1, fig 3 CHEBI:36274 is required for the binding of Q15788 to Q96RI1-2 CHEBI:36274 -glycochenodeoxycholic acid ncoa1-nr1h4-1 1 7 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 stimulator stimulator unspecified role unspecified role 3 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:15:28 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'glu685ala' region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Table 1, fig 3 taurochenodeoxycholic acid CHEBI:16525 CHEBI:16525 is required for the binding of Q15788 to Q96RI1-2 ncoa1-nr1h4-11 1 7 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 stimulator stimulator unspecified role unspecified role 3 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:10:42 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'glu685ala' direct interaction direct interaction Table 1, fig 3 CHEBI:90999 CHEBI:90999 is required for the binding of Q15788 to Q96RI1-2