IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-neuron Human neurons neuron Neuron [Neuronal cell; Neural cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. A monclonal antibody was used to detect Htt. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. Some participants were visualised using Tag fluorescence. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. Fibroblasts were used from a control or Huntingdon's disease patient. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. Mol. Cell (1097-2765) 2010 imex curation Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2A cannot be annoted - nucleporins are a family of proteins. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Mol. Cell (1097-2765) 2010 jyoti Only protein-protein interactions imex curation atx1_human Ataxin-1 ATXN1 ATX1 SCA1 Spinocerebellar ataxia type 1 protein protein protein human Homo sapiens Human MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK DIP protein P54253 original sequence version: 102 DIP protein P54253 original sequence version: 135 657876F8FD19ECB2 atg12_mouse Ubiquitin-like protein ATG12 Atg12 Apg12 Apg12l Autophagy-related protein 12 protein protein mouse Mus musculus Mouse MSEDSEVVLQLPSAPVGAGGESLPELSPETATPEPPSSAAVSPGTEEPPGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG DIP protein Q9CQY1 original sequence version: 111 440F9A625624A78B atg5_rat Autophagy protein 5 Atg5 protein protein rat Rattus norvegicus (Rat) Rat MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDVSEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPCKDAVEAHFMSCVKEADALKHKSQVINEMQRKDHKQLWMGLQNDRFDQFWTINRKLMEYPPEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLREVCPSAVAPEDGEKKSQVMIHGIEPLLETPLQWLSEHLSYPDNFLHISIVPQPTD 1F6EDC95C7870105 wdfy3_mouse WD repeat and FYVE domain-containing protein 3 Wdfy3 Beach domain, WD repeat and FYVE domain-containing protein 1 protein protein mouse Mus musculus Mouse MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPNTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKTVDCMTTMSVPSTLVKCLYLFFDLPHVPEAGGGAQNELPLAERRGLLQKAFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSSDVSQTLLDDFRIWQGYNFLCDLLLRLEQGKEAECRDALKDLVSLVTSLTTYGVSELKPAGVTTGAPFLLPGFAVPQPAGKGHSVRNIQAFAVLQNAFLKAKTNFLAQIILDAITNIYMADNANYFILESQHTLSQFAEKISKLPEVQNKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSYHCSIIAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPAQALNEQGDSRNNSSVEDQKHLALLVMEALTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGEDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSSPPKNGWEKVSQSQVLELLHTVFCTLTAALRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAVNVFPSNTQPFQRLLEEGAVSVDSVSPTLRHCSKLFIYLYKVATDSFDSHAEQIPPCLTSESSLPSPWGTPALSRKRHAFHCVSTPPVYPAKNVTDLKLQVTSSPLQSSDAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCGAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSFEPEMRSSVITSLEGLGSDNVFSSHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNTVTTGLTDGAVVSGMGSGERFFPPPSGLSYSCWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYLDGQLVSTVKLHYVHSTPGGSGSANPPVLSTVYAYVGTPPAQRQIASLVWRLGPTHFLEEVLPPSSVTTIYELGPNYVGSFQAVCVPCKDAKSEGVTPSPVSLVAEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLVHNAAGHLNGPARTIGAALIGYLGVRTFVPKPVATTLQYIGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKKRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAISNVLSFLLQGFPNSNDLLRFGQFISSTLPTFAVCEKFVVMEINNEEKPDPGAEEEFGGLVSANLILLRNRLLDILLKLVYTSKEKTNINLQACEELVRTLGFDWIMMFMEEHLHPTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFLVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVTSSRCKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTESAFLLLGMLRSMLNSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWLSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTHVLDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHAGSVEGFGLEAEARMTTWHIMIPSDIEPDGGYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEELFKVSLPVNDRGHVDIALARPLIEEAGLKCWQNHLAHEKKCISRGEALVPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLHKHSPSPQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQEASVGKPARYRRAISYDSKEYYLRLASGNPAIVQDAIVESSEGEATQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEETQEQLQDQIAESGSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILSDYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGDNIGISVPPGATSDKIFFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPAWNKTFAWGYADLSCRLGTYESDKAVTVYECLSEWGQILCAVCPNPKLVITGGTSTVVCVWEMGTSKEKAKPLTLKQALLGHTDTVTCATASLAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGRSQQIVCCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPVEDLEMQEGCPEAQIGQQAQDDDSSDSETEEPSVSQDPKDTSSQPSSTSHRPRAASCRATATWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQTRAHLQGPLAHPHPNPIEARSYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNTHPAEVTALGVSKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYSLQHERGAEDGPRNC 9718CB1AABCB3B8E atg5_mouse Autophagy protein 5 Atg5 Apg5l APG5-like protein protein mouse Mus musculus Mouse MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDVSEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAVEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPPEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLREVCPSAVAPEDGEKRSQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIVPQPTD DIP protein Q99J83 original sequence version: 54 F3FCE652D627B694 wdfy3_human WD repeat and FYVE domain-containing protein 3 Autophagy-linked FYVE protein WDFY3 KIAA0993 protein protein human Homo sapiens Human MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPNTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKTVDCMTTMSVPSTLVKCLYLFFDLPHVPEAVGGAQNELPLAERRGLLQKVFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSSDVSQTLLDDFRIWQGYNFLCDLLLRLEQAKEAESKDALKDLVNLITSLTTYGVSELKPAGITTGAPFLLPGFAVPQPAGKGHSVRNVQAFAVLQNAFLKAKTSFLAQIILDAITNIYMADNANYFILESQHTLSQFAEKISKLPEVQNKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSYHCSIIAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPTQALNEQGDSRNNSSVEDQKHLALLVMETLTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGDDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSCPPKNGWEKVNQNQVFELLHTVFCTLTAAMRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAMNVFPSNTQPFQRLLEEDVISIESVSPTLRHCSKLFIYLYKVATDSFDSRAEQIPPCLTSESSLPSPWGTPALSRKRHAYHSVSTPPVYPPKNVADLKLHVTTSSLQSSDAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCSAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSYEPEMRSSMITSLEGLGTDNVFSLHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNTVTTGLIDGAVVSGIGSGERFFPPPSGLSYSSWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVKLHYVHSTPGGSGSANPPVVSTVYAYIGTPPAQRQIASLVWRLGPTHFLEEVLPSSNVTTIYELGPNYVGSFQAVCMPCKDAKSEGVVPSPVSLVPEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLIHNSAGHLNGSARTIGAALIGYLGVRTFVPKPVATTLQYVGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKKRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAISNVLSFLLQGFPSSNDLLRFGQFISSTLPTFAVCEKFVVMEINNEEKLDTGTEEEFGGLVSANLILLRNRLLDILLKLIYTSKEKTSINLQACEELVKTLGFDWIMMFMEEHLHSTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFPVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVISCRSKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTEAVFLLLGMLRSMLTSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWMSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTYILDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHVGSNVDGFGLEAEARMTTWHIMIPSDIEPDGSYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEELFKVTLPVNERGHVDIATARPLIEEAALKCWQNHLAHEKKCISRGEALAPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLNKHSLSTQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQETNVASEIPSKQPETPDDIPQKKPARYRRAVSYDSKEYYMRLASGNPAIVQDAIVESSEGEAAQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEENQEQLQDQIAEGSSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILADYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGDNAGISVLPGSTSDKIFFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPTWNKTFAWGYADLSCRLGTYESDKAMTVYECLSEWGQILCAICPNPKLVITGGTSTVVCVWEMGTSKEKAKTVTLKQALLGHTDTVTCATASLAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGRSQQIICCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPAEVLEMQEDCPEAQIGQEAQDEDSSDSEADEQSISQDPKDTPSQPSSTSHRPRAASCRATAAWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQTRAHLQGPLSHPHPNPIEVRNYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNAHPAEVTALGISKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYNLQHERGSEDGPRNC F4D518E8C9C12E23 a16l1_human Autophagy-related protein 16-1 APG16-like 1 UNQ9393/PRO34307 ATG16L1 APG16L protein protein human Homo sapiens Human MSSGLRAADFPRWKRHISEQLRRRDRLQRQAFEEIILQYNKLLEKSDLHSVLAQKLQAEKHDVPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAECLQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTTEENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFGRRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVAWSPSGSHVVSVDKGCKAVLWAQY DIP protein Q676U5 original sequence version: 96 5A5816AE2CF03CA0 nbr1_human Next to BRCA1 gene 1 protein Neighbor of BRCA1 gene 1 protein Membrane component chromosome 17 surface marker 2 Protein 1A1-3B NBR1 1A13B KIAA0049 M17S2 Cell migration-inducing gene 19 protein MIG19 protein protein human Homo sapiens Human MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVPCDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRLPAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLTLASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNIEKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDLLSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKAENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEAVMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGVEAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPVTIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQPIISEDQTAALMAHLFEMGFCDRQLNLRLLKKHNYNILQVVTELLQLNNNDWYSQRY 6A057E67947838EF sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD sqstm_rat Sequestosome-1 Sqstm1 Zip Protein kinase C-zeta-interacting protein Ubiquitin-binding protein p62 protein protein rat Rattus norvegicus (Rat) Rat MASLTVKAYLLGKEEAAREIRRFSFCFSPEPEAEAAAGPGPCERLLSRVAVLFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEARSMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPSEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL E611F296E2B11464 syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA DIP protein P37840 original sequence version: 124 DIP protein P37840 original sequence version: 175 DIP protein P37840 original sequence version: 178 6BB2F12128931663 atg5_human Autophagy protein 5 APG5-like Apoptosis-specific protein ATG5 APG5L ASP protein protein human Homo sapiens Human MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD DIP protein Q9H1Y0 original sequence version: 63 DIP protein Q9H1Y0 original sequence version: 56 DIP protein Q9H1Y0-1 original sequence version: 105 DIP protein Q9H1Y0 original sequence version: 105 C33A1E0B3C1DBE5C hd_human Huntingtin HTT IT15 HD Huntington disease protein protein protein human Homo sapiens Human MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC DIP protein P42858 original sequence version: 148 DIP protein P42858 original sequence version: 145 DIP protein P42858 original sequence version: 142 DIP protein P42858 original sequence version: 157 DIP protein P42858 original sequence version: 158 DIP protein P42858 original sequence version: 156 A267509E84D52F0D d4a7d8_rat Wdfy3 protein protein rat Rattus norvegicus (Rat) Rat MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPSTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKAVDCMTTMSVPSTLVKCLYLFFDLPHVPEAGGGAQSELPLAERRGLLQKAFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSNSLSSRFLDCFLKKKNIRYFCKYFIQLEQAKEAECRDALKDLVSLVTSLTTYGVSELKPAGVTTGAPFLLPGFSVPQPAGKGHSVRNIQAFAVLQNAFLKAKTNFLAQIILDAITDIYMADSANYFILESQHTLSQFAEKISKLPEVQSKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSHHCSIVAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPAQALGEQGDSRNNSSVEDQKHLALLVMEALTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGEDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSSPPKNGWEKVNQNQVFELLHTVFCTLTAALRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAVNVFPSNTSPFQRLLEEGAVPMDSVSPTLRHCSKLFIYLYKVATDSFDSRTEQIPPCLTSESSLPSPWGTPALSRKRHAFHCVSTPPVYPAKSVTDLKLQVTSSPLPSSEAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCGAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSFEPDMRSSVVTSLEGLGSDSVFSSHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNAVTTGLTDGAVVSGIGSGERFFPPPSGLSYSCWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYIDGQLVSTVKLHYVHSTPGGSGSANPPVLSTVYAYIGTPPAQRQMASLVWRLGPTHFLEEVLPPSSVTAIYELGPDYVGSFQAVLVPCKDSKSEGIIPSPVSLVAEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLVHNAAGHLNGPARTIGAALIGYLGVRTFVPKPVATTLQYIGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKRRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAMSNVLSHLLQGFPNSNDLLRFGQFISSTLPTFAVCEKFVVMEIINEGKPDSGAEEEFGGLVSANLILLRNRLLDILLKLVYTSKEKTNINLQACEELVRTLGFDWIMMFMEEHLHPTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFLVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVTNSRCKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTESAFLLLGMLRSMLNSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWLSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTHILDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHAGSSVEGFGLEAEARMTTWHIMIPSDIEPDGGYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEEVFKVSLPVNDRGHVDIAVARPLIEEAGLKGWQNHLAHEKKCISRGEALVPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLHKHSPSPQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQEASVGKPARYRRAISYDSKEYYLRLASGNPTIVQDAIVESSEGEATQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEETQEQLQDQIAESGSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILADYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGENAGLSVPPGATSDKXFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPAWNKTFAWGYADLSCRLGTYESDKAVAVYECLSEWGQILCAVCPSPKLVITGGTSTVVCVWEMGTSKEKAKPLTLKQALLGHTDTVTCATASIAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYVHVWSINGNPIVSVNTFTGRSQQIVCCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPVEDLEMQEGCPEAQIGQQAQDEDSSDSEAEEPSVSQDPKDTPSQPSSTSHRPRAASCRATATWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQARAHPQGSHSHPNPTEARSYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNTHPAEVTALGVSKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYSLQHERGADDGPRNC The sequence has been withdrawn from uniprot. A7B7CEDF22562D5B mlp3b_rat Microtubule-associated proteins 1A/1B light chain 3B Map1lc3b Map1alc3 Map1lc3 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 light chain 3 beta protein protein rat Rattus norvegicus (Rat) Rat MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTALAVTYMSALKATATGREPCL D07E9D063C125E32 atg12_human Ubiquitin-like protein ATG12 Autophagy-related protein 12 ATG12 APG12 APG12L protein protein human Homo sapiens Human MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG DIP protein O94817 original sequence version: 63 DIP protein O94817-1 original sequence version: 102 109258521C2D194F mlp3b_human Microtubule-associated proteins 1A/1B light chain 3B MAP1LC3B MAP1ALC3 Microtubule-associated protein 1 light chain 3 beta MAP1A/MAP1B light chain 3 B MAP1 light chain 3-like protein 2 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B protein protein human Homo sapiens Human MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV DIP protein Q9GZQ8 original sequence version: 69 DIP protein Q9GZQ8 original sequence version: 108 DIP protein Q9GZQ8 original sequence version: 129 DIP protein Q9GZQ8 original sequence version: 132 DIP protein Q9GZQ8 original sequence version: 148 DIP protein Q9GZQ8 original sequence version: 152 DIP protein Q9GZQ8 original sequence version: 111 DIP protein Q9GZQ8 original sequence version: 153 BB141DEC7653E83F atg5-alfy 4 17 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4B. IPR011046 added as least inappropriate domain for Alfy. alfy-httq68 11 24 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_q68 Exon1 amino acids from Htt with 68 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 2B. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I got an error message when trying to enter a range of 1-?. The reference given - Bjorkoy et al. 2005, states the construct used contained Htt amino acids 1-171. However this paper states the Htt sequence was taken from Exon 1 only, which is amino acids 1~90 (including the 21 glutamines residues according to P42858) - this would give a total number of ~140 amino acids used from Htt in this construct, rather than 171. atg5-alfy-wt 11 17 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait colocalization colocalization Fig 4A panel 1. gst_atg5-mbp_alfy 3 17 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 4C. Constructs MBP-Alfy2888-3526 = MBP-AlfyCT639 - as 3526 minus 2888 = ~639 atg5-alfy-2 7 17 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait physical association physical association Fig 4D. alfy-atg5 8 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Fig 4E. htt103q_atg16_atg12 4 27 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_q103 Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 18 unspecified role unspecified role prey prey association association Fig 4F. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. htt65q 4 17 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range association association Fig 5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. LC3 molecule was identified by blasting the sequence given in the Kabeya-2000 Reference. Image and the image legend do not give the same antibodies - ones on the image have been entered, only. htt103q- 4 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 28 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey association association Fig 5A, 5D. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. (Human) LC3 molecule was identified by blasting the sequence given in the Kabeya-2000 Reference. Image and the image legend do not give the same antibodies - ones on the image have been entered, only. Fig 5 (D+E) image legend states "Experiments were performed similarly to (B)" - but these look much more like "A" to me. Alfie knockdown (si-Alfy) diminished the interaction with Atg5, and a diminished amount of LC3 was present in the complex. htt72q-wdfy3 1 24 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 23 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey association association Fig 5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. LC3 molecule was identified by blasting the sequence given in the Kabeya-2000 Reference. Image and the image legend do not give the same antibodies - ones on the image have been entered, only. htt103q-1 4 17 unspecified role unspecified role prey prey n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association Fig 5B. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The image legend states the c-terminal sequence of Alfy is aa 2543-3527, but the protein ends at aa 3526. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. Both the N- and C-terminal fragments of Alfy can interact with the polyQ protein. htt103q-2 10 24 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey association association Fig 5C. LC3 molecule was identified by blasting the sequence given in the Kabeya-2000 Reference. Immunoprecipitation with Alfy revealed that endogenous Htt comes down with Alfy only in HD patient samples. htt72q-1 9 21 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 26 unspecified role unspecified role prey prey association association Fig 5E. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. The Rat Alfy Trembl entry (D4A7D8) will presumably be "uprated" to something other than "predicted"? (I dont have to DO anything, do I?). Alfie knockdown (sh-Alfy) diminished the interaction with Atg5, and a diminished amount of LC3 was present in the complex. htt65q-1 11 19 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range colocalization colocalization Fig 5F. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. NO cellular location is given for the co-localisation w.r.t. this image. htt65q-2 4 19 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association Fig 5G. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. htt103q-3 4 19 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association Fig 5G. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The image legend states the c-terminal sequence of Alfy is aa 2543-3527, but the protein ends at aa 3526. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. alfy-httq72_or_79 2 17 unspecified role unspecified role neutral component neutral component td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 24 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_q72 Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range colocalization colocalization Fig 6A, S7C. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The number of glutamines is stated to be 79 on the image, but 72 on the image legend. Expression of Alfy2981-3526 led to a significant reduction in the aggregate load in the neurons. Overexpression of the full-length 400 kDa Alfy was very difficult to achieve, but in the few cells that were successfully transfected, the frequency of inclusions was fewer, as for Alfy2981-3526. as far as I can see the 2 figures look to contain the same data except the full length version is in S7 only. alfy-httq68-1 6 17 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_q68 amino acids from Htt with 68 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 26 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig S1A. atg5-htt 6 23 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range colocalization colocalization Fig S5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. htt103q_alfy 5 24 unspecified role unspecified role neutral component neutral component c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 17 unspecified role unspecified role neutral component neutral component n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain td_tomato tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig S6A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. Both the N- and C-terminal fragments of Alfy interact with the polyQ protein:The N-terminal fragment completely colocalized with the Htt aggregate, while the C-terminal fragment was only found to decorate the outside of the inclusion. htt72q-2 8 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association Fig S6B. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. I have entered the possible range of amino acids for the C terminus of the Htt sequence used.- I previously got an error message when trying to enter a range of 1-?. Alfy can IP in the presence of polyQ proteins in ATG5 knockout cells. td_alfy-afg5 6 23 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig 4A panels 2, 4 and 6. I have entered the suff. to bind as aa 2981-3526, as the yellow colour in panel 6 six looks to be reduced. No construct mentioning aas 2981-3311 - as used in panel 6, is mentioned in the Supplement or main body of the paper. I have added the InterPro domain aas 2840 - 3439 as the least inappropriate - several other contenders possible. alfy-ataxin1 11 12 unspecified role unspecified role neutral component neutral component P54253:p.Lys771Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K T Wed May 02 15:12:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys771thr' Tue Jun 30 15:53:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys771Thr' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig S 2C/D. Ataxin1 (Q82) containing the nuclear localisation signal mutation K772T did not colocalize in the nucleus with Alfy, though some cytoplasmic colocalisation can be seen. a_syn_ 4 22 unspecified role unspecified role bait bait region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey association association Fig S6E. (Human) LC3 molecule was identified by blasting the sequence given in the Kabeya-2000 Reference. Image and the image legend do not give the same antibodies - ones on the image have been entered, only. This must be an anti-TAG IP as there are no mention of any antibodies to alph-syn.