IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers. Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers. Nature structural & molecular biology 2020 imex curation Mandala VS., McKay MJ., Shcherbakov AA., Dregni AJ., Kolocouris A., Hong M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus meihong@mit.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid state nmr solid state nmr ssnmr solid state nmr predetermined predetermined participant predetermined Mandala VS., McKay MJ., Shcherbakov AA., Dregni AJ., Kolocouris A., Hong M. Virus - Publications including interactions involving viral proteins SARS-CoV-2 virus, envelope protein E transmembrane domain (ETM) was reconstituted in phospholipid bilayers. meihong@mit.edu Accepted 2021-FEB-03 AT 12:54 GMT AT 12:54 GMT by MARTA Nature structural & molecular biology 2020 Only protein-protein interactions imex curation imex curation 5-(n,n-hexamethylene)amiloride 5-(N,N-hexamethylene)amiloride HMA small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) amantadine amantadine 1-adamantanamine 1-adamantylamine 1-aminoadamantane amantadina amantadine Amantadine amantadinum Amantidine Aminoadamantane tricyclo[3.3.1.1(3,7)]decan-1-amine tricyclo[3.3.1.1(3,7)]decan-1-ylamine tricyclo[3.3.1.1(3,7)]decane-1-amine Viregyt Virosol adamantan-1-ylamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) DKNWSYNQZKUICI-UHFFFAOYSA-N DKNWSYNQZKUICI-UHFFFAOYSA-N vemp_sars2 Envelope small membrane protein E 4 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV e-1 1 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2D, Table 1, supp. Tables 1+2. e-e-3 1 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4'-19fluoro-L-phenylalanine isotope label isotope label certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2, Extended Data 5, supp. Table 3 An equimolar mixture of 13C-labeled protein and 4-19F-Phe-labeled protein was used to measure intermolecular 13C-19F distances using the rotational-echo double-resonance (REDOR) technique. A single pentamer model, characterized by having Asn15 and Val25 at similar pore-facing orientations and all three Phe residues facing lipids, was found to best describe the experimental data. as i cannot add a pentamer with 2 different proteins, I have just added the comment "..experimental data." 5_(n,n_hexamethylene)amiloride-e-1 1 2 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 The CSPs (Chemical Shift Perturbations) of HMA are larger than those of AMT. amantadine-e-1 1 3 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 4C, Extended Data Fig. 10 amantadine-e-2 1 3 unspecified role unspecified role neutral component neutral component 3-19Fluorine isotope label isotope label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component purified purified transmembrane domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Extended Data Fig. 10