IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Science advances 2020 imex curation Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus Virus - Publications including interactions involving viral proteins The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Accepted 2020-SEP-29 AT 17:10 BST AT 17:10 BST by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Science advances 2020 imex curation Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus Virus - Publications including interactions involving viral proteins The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Accepted 2020-SEP-29 AT 17:10 BST AT 17:10 BST by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Science advances 2020 imex curation Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus Virus - Publications including interactions involving viral proteins The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk 13-Nov-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel sequence tag sequence tag identification sequence tag Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus A fraction from H1N1 virus-infected MDCK cells was incubated with azido-ACA, and further incubated with DyLight 488-Phosphine to allow linkage of fluorescent dye with the azido group. The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Accepted 2020-SEP-29 AT 17:10 BST AT 17:10 BST by KALPANAP Science advances 2020 Only protein-protein interactions imex curation imex curation e2quu5_canlf 60 kDa chaperonin 60 kDa heat shock protein, mitochondrial Chaperonin 60 Heat shock protein 60 HSPD1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MLRLPAVLRHVRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSITPANEDQRIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF 9E8BCAD3F40CBFD5 f1ppt2_canlf KIF21A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MFFITQHVTSCMWIITTELLSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIIEEEQGIISRAVKHLFKSIEEKKHTAIKNGHPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHLCQTRMCPQTDAENATDNKVISESSQLNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESNREERSVASKEDNTDTDQEKKEEKGITERENNELEVEESQEVSDHEEEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVRSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDRVAGKVTRKLSSSDTPVQDMGSTAAAVEADVSRAGAQQKMRIPVARVQALPTPTTNGTRKKYQRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLKQREELTKRREKLSKRREKIVKESEEGDKNVININEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEEAPLNSPGSEGSTLSSDLMKLCGEVKPKSKARRRTTTQMELLYADSSELASDTSTGDASLPGPLTPVAEGQEIGMNTETSGTSAREKELPPPSGFPSKIGSISRQSSLSEKKMPEPSPITRRKAYEKAEKSKAKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHSRSSRRGIINPFPASKGIRTSPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIVTGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPVLLSGCRGGILKLWNMDTFVPVGEMKGHESPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDIGDLGEDIASN 12E408EFA229C69A e2rcj9_canlf CNTN1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MKMWLLVSHLVIISFTSCLSEFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESPEGKVSLNCRARASPFPVYKWRMNNGDIDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPLPERTTKPYPADIVVQFKDIYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGTYECEAENNRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTHGTIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGRANNTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFIWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGIGEPSIPSNKIKTDGAAPNVAPSDVGGGGGSNRELTITWVPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMRPSTAFQVKVKAFNNKGDGPYSLTAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVVEKIVESYQIRYWAAHDKEAAAHRVQVTSQEYSARLENLLPDTQYFVEVRACNSAGCGPPSDMIETFTKKAPPSQPPRIISSIRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKISGVSTLSPTLLGLLLPALGIVVYLAI CF7CBD8DA178BBB1 e2r8d3_canlf Vasopressin V1a receptor AVPR1A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRIPGGPGAPSAGNSSRWWPASGRGANASGDAGALADGGGPPRDARNEELAKLEIAVLAVTFVVAVLGNSSVLVALHRTPRKTSRMHLFIRHLSLADLAVAFFQVLPQMCWDITYRFRGPDGLCRVVKHLQVFGMFVSPYMLVVMTADRYIAVCHPLKTLQQPTRRSRLMIAAAWVLSLVLSTPQYLVFSMVEVNNVTKANDCWATFIQPWGPRAYVTWMTAGIFVAPVVLLATCYGCICSHIWRSVRGRTALRAGAAAGAVPRGVLRAPGASSVKTISRAKMRTVKMTFVIVTVYIVCWAPFFILQMWSVWDDGFVWIESENPAITITALLASLNSCCNPWIYMFFSGHLLQDCVQSFPCCQNMKQTFNKVDSDSVSRRQTSYTNNRSPTNSMGTWKDSPKTSKSVKFIPVST 2D0E9C25E11D3A97 n-(p-amylcinnamoyl)anthranilic acid N-(p-amylcinnamoyl)anthranilic acid 4-amylcinnamoylanthranilic acid ACA p-amylcinnamoylanthranilic acid 2-{[(2E)-3-(4-pentylphenyl)prop-2-enoyl]amino}benzoic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) calr_canlf Calreticulin CRP55 Calregulin Endoplasmic reticulum resident protein 60 HACBP CALR protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris EPAIYFKEQFLDGXGFTDXRIKEK D5C76F2CB94B5C40 yxxø DYQRLN peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DYQRLN ap2m1_human AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain AP2M1 CLAPM1 KIAA0109 Adaptin-mu2 Plasma membrane adaptor AP-2 50 kDa protein HA2 50 kDa subunit Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein human Homo sapiens Human MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC 82803219BA279954 f1pk90_canlf RNA helicase DDX46 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKAENRSRSKEKTDSGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL F64C8689558976A7 a0a455r0b8_canlf TMPRSS2 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPLNSGSPPGVGPYYENHGYQPESLYPPQPATAPRAYPVYPAPYYPPAVPQYTPRVLTHTSTPAIHTQPKSPLGTGCTAKTKKALCITVVLGTILAGAAVAAVLLWKFMEDKCSVSGIECGSSGTCISPSQWCDGVLHCPSGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDNWSDSYGRAACQDMGYRNSFYSSQGIADDSGATSFMKLNISAGHMDLYKKLYHSDVCSSKTVVSLRCIECGVSAKGSRQSRIVGGTSASLGDWPWQVSLHVQGTHVCGGSIISPEWIVTAAHCVEEPLNNPRYWTAFAGILRQSFMFYGHGHRVGKVISHPNYDSKTKNNDIALMKLQTPLTFNDRVKPVCLPNPGMMLEPEQSCWISGWGATYEKGKTSDELNAVMVPLIEPWRCNSKYVYNNLVTPAMICAGFLRGGVDSCQGDSGGPLVTLKSRVWWLIGDTSWGSGCAKANRPGVYGNVTVFTDWIYRQMRANS 2AA5B0AA98A01BD4 ncap_mers1 Nucleoprotein N Nucleocapsid protein 6 protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC MASPAAPRAVSFADNNDITNTNLSRGRGRNPKPRAAPNNTVSWYTGLTQHGKVPLTFPPGQGVPLNANSTPAQNAGYWRRQDRKINTGNGIKQLAPRWYFYYTGTGPEAALPFRAVKDGIVWVHEDGATDAPSTFGTRNPNNDSAIVTQFAPGTKLPKNFHIEGTGGNSQSSSRASSVSRNSSRSSSQGSRSGNSTRGTSPGPSGIGAVGGDLLYLDLLNRLQALESGKVKQSQPKVITKKDAAAAKNKMRHKRTSTKSFNMVQAFGLRGPGDLQGNFGDLQLNKLGTEDPRWPQIAELAPTASAFMGMSQFKLTHQNNDDHGNPVYFLRYSGAIKLDPKNPNYNKWLELLEQNIDAYKTFPKKEKKQKAPKEESTDQMSEPPKEHRVQGTQRTRTRPSVQPGPMIDVNTD 8AF44237C8308608 q32ze1-pro_0000435838 Serine protease NS3 protein protein zikv Zika virus (strain Mr 766) ZIKV SGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMWHVTKGAALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGLSEVQLLAVPPGERARNIQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAITQGKREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKKRLRTVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLNIMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKTKNQEWDFVITTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPNKPGDEYMYGGGCAETDEGHAHWLEARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIMEDSVPAEVWTKYGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKR 1499 2115 30EA1BE527A33104 b9vuu3-pro_0000449080 Protein 2C protein protein EV71 Human enterovirus 71 EV-71 SASWLKKFNDMANAAKGLEWVSNKISKFIDWLKEKIVPAAKEKVEFLNNLKQLPLLENQISNLEQSAASQEDLEVMFGNVSYLAHFCRKFQPLYATEAKRVYALEKRMNNYMQFKSKHRIEPVCLIIRGSPGTGKSLATGIIARAIADKYHSSVYSLPPDPDHFDGYKQQVVTVMDDLCQNPDGKDMSLFCQMVSTVDFIPPMASLEEKGVSFTSKFVIASTNATNIIVPTVSDSDAIRRRFYMDCDIEVTDSYKTDLGRLDAGRAAKLCSENNTANFKRCSPLVCGKAIQLRDRKSKVRYSVDTVVSELIREYSNRSAIGNTIEALFQ 1112 1440 142AF9D060BC6ED4 ncap_i34a1 Nucleoprotein NP Nucleocapsid protein protein protein i34a1 Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDN DIP protein P03466 original sequence version: 99 4F750FEF05D6E668 a0a5f4bq86_canlf AP-2 complex subunit mu AP2M1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC 82803219BA279954 ap2m1-avpr1a-1 3 13 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 5 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 12 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 6 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 9 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 18 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line 8 unspecified role unspecified role neutral component neutral component azido group tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DyLight 488 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 29 30 association association 4D Gel plug of the target band was collected for LC-MS/MS, which identified eight candidate proteins that were physically associated with azido-ACA. ap2m1-yxx-1 2 10 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role bait bait cell lysate cell lysate Q96CW1:p.Asp176Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Fri Sep 18 22:20:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp176Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 5B, number 1 ap2m1-yxx-2 2 10 competitor competitor competitor prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role bait bait cell lysate cell lysate region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 5B, number 2 ap2m1-yxx-3 2 8 inhibitor inhibitor prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role bait bait cell lysate cell lysate region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q96CW1:p.Asp176Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Fri Sep 18 22:04:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp176Ala' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 5B, number 3 the pink and blue columns look to have the same treatment (ACA only added) to me? ap2m1-np-1 1 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Figure 7A ACA -CHEBI:139349 . ap2m1-np-2 1 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Figure 7B ACA -CHEBI:139349 . ap2m1-n-1 1 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Figure 7C ACA -CHEBI:139349 . ap2m1-n-2 1 16 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Figure 7D ACA -CHEBI:139349 .