IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus. Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus. The Journal of biological chemistry 2012 imex curation Wu K., Peng G., Wilken M., Geraghty RJ., Li F. Coronavirus - Interactions investigated in the context of Coronavirus lifang@umn.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wu K., Peng G., Wilken M., Geraghty RJ., Li F. Coronavirus - Interactions investigated in the context of Coronavirus Viral rbds are taken from refs 26/7. The identity of the civet ACE2 molecule was taken from the PDBe entries. lifang@umn.edu Accepted 2020-APR-07 AT 11:08 BST AT 11:08 BST by ORCHARD The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus. Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus. The Journal of biological chemistry 2012 imex curation Wu K., Peng G., Wilken M., Geraghty RJ., Li F. Coronavirus - Interactions investigated in the context of Coronavirus lifang@umn.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wu K., Peng G., Wilken M., Geraghty RJ., Li F. Coronavirus - Interactions investigated in the context of Coronavirus viral rbds are not mentioned in the text - only refs 26/7 and these are only the same as PDBe ones for one entry. I know we do not normally enter chimeric proteins, but I had got half way through these before I saw the ACE2 was chimeric, and added for the sake of completeness. Viral RBDs taken from PDBe entries as are all sequences. lifang@umn.edu Accepted 2020-APR-07 AT 11:08 BST AT 11:08 BST by ORCHARD The Journal of biological chemistry 2012 Only protein-protein interactions imex curation imex curation spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 ace2_pagla Angiotensin-converting enzyme 2 ACE2 ACE-related carboxypeptidase protein protein Masked palm civet Paguma larvata MSGSFWLLLSFAALTAAQSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVTIWLIVFGVVMGAIVVGIVLLIVSGIRNRRKNDQAGSEENPYASVDLNKGENNPGFQHADDVQTSF CF6406851F73E378 ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 ace2-s-13 2 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P59594:p.[Tyr442Phe;Leu472Phe] mutation mutation certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 16:01:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr442Phe;Leu472Phe]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1, number 1 ace2-s-14 2 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role neutral component neutral component purified purified P59594:p.[Leu472Pro;Asn479_Asp480delinsArgGly] mutation mutation certain certain sequence position certain certain certain sequence position certain ND RG certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 20:03:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu472Pro;Asn479_Asp480delinsArgGly]' C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1, number 2 ace2-s-15 2 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Ile21Thr;Gln24Leu;Asp30_Lys31delinsGluThr;His34Tyr;Glu37_Asp38delinsGlnGlu;Phe40Ser;Leu45Val;Met82Thr] mutation mutation certain certain sequence position certain certain certain sequence position certain M T certain certain sequence position certain certain certain sequence position certain I T certain certain sequence position certain certain certain sequence position certain Q L certain certain sequence position certain certain certain sequence position certain DK ET certain certain sequence position certain certain certain sequence position certain H Y certain certain sequence position certain certain certain sequence position certain ED QE certain certain sequence position certain certain certain sequence position certain F S certain certain sequence position certain certain certain sequence position certain L V Tue Jun 30 18:55:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile21Thr;Gln24Leu;Asp30_Lys31delinsGluThr;His34Tyr;Glu37_Asp38delinsGlnGlu;Phe40Ser;Leu45Val;Met82Thr]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role neutral component neutral component purified purified P59594:p.[Leu472Pro;Asn479_Asp480delinsArgGly] mutation mutation certain certain sequence position certain certain certain sequence position certain ND RG certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 19:40:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu472Pro;Asn479_Asp480delinsArgGly]' C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1, number 3 ACE2 sequence is stated by PDBe to be partially that of the "Paguma larvata (Masked palm civet)". (Q56NL1) ace2-s-16 2 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Ile21Thr;Gln24Leu;Asp30_Lys31delinsGluThr;His34Tyr;Glu37_Asp38delinsGlnGlu;Phe40Ser;Leu45Val;Met82Thr] mutation mutation certain certain sequence position certain certain certain sequence position certain I T certain certain sequence position certain certain certain sequence position certain Q L certain certain sequence position certain certain certain sequence position certain DK ET certain certain sequence position certain certain certain sequence position certain H Y certain certain sequence position certain certain certain sequence position certain ED QE certain certain sequence position certain certain certain sequence position certain F S certain certain sequence position certain certain certain sequence position certain L V certain certain sequence position certain certain certain sequence position certain M T Tue Apr 28 10:29:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile21Thr;Gln24Leu;Asp30_Lys31delinsGluThr;His34Tyr;Glu37_Asp38delinsGlnGlu;Phe40Ser;Leu45Val;Met82thr]' Tue Jun 30 17:12:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile21Thr;Gln24Leu;Asp30_Lys31delinsGluThr;His34Tyr;Glu37_Asp38delinsGlnGlu;Phe40Ser;Leu45Val;Met82Thr]' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1, number 4 ACE2 sequence is stated by PDBe to be partially that of the "Paguma larvata (Masked palm civet)". (Q56NL1) ace2-s-17 1 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.Lys353Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 20:07:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Ala' Q9BYF1:p.Lys31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:56:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys31Ala' Q9BYF1:p.Lys31Thr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K T Tue Jun 30 20:07:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys31Thr' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Figure 2A s-ace2-5 1 5 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey purified purified P59594:p.Asn479Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N R Tue Jun 30 18:33:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Arg' P59594:p.Leu472Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 20:18:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu472Pro' P59594:p.Asp480Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D G Tue Jun 30 20:20:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp480Gly' P59594:p.Asn479Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 17:11:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.Thr487Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 19:49:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' P59594:p.[Tyr442Phe;Leu472Phe] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 20:15:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr442Phe;Leu472Phe]' P59594:p.Leu472Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 16:45:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu472Phe' P59594:p.[Leu472Pro;Asn479_Asp480delinsArgGly] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L P certain certain sequence position certain certain certain sequence position certain ND RG Tue Jun 30 17:24:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu472Pro;Asn479_Asp480delinsArgGly]' C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P59594:p.Tyr442Phe mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 19:03:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr442Phe' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Figure 3A ace2-s-18 1 3 unspecified role unspecified role prey prey purified purified P59594:p.Asn479Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N R Tue Jun 30 18:48:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Arg' P59594:p.Leu472Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L P Tue Jun 30 17:08:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu472Pro' P59594:p.Asp480Gly mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D G Tue Jun 30 16:52:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp480Gly' P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 17:40:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.Thr487Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 20:11:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' P59594:p.[Tyr442Phe;Leu472Phe] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 15:51:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr442Phe;Leu472Phe]' P59594:p.Leu472Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L F Tue Jun 30 16:03:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu472Phe' P59594:p.[Leu472Pro;Asn479_Asp480delinsArgGly] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain L P certain certain sequence position certain certain certain sequence position certain ND RG Tue Jun 30 17:29:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu472Pro;Asn479_Asp480delinsArgGly]' C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P59594:p.Tyr442Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F Tue Jun 30 20:21:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr442Phe' spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 4 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Figure 3B