DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Modular organization of SARS coronavirus nucleocapsid protein. Modular organization of SARS coronavirus nucleocapsid protein. Journal of biomedical science 2006 imex curation Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Accepted 2020-JUN-18 AT 08:05 BST AT 08:05 BST by ORCHARD Journal of biomedical science 2006 imex curation Modular organization of SARS coronavirus nucleocapsid protein. Modular organization of SARS coronavirus nucleocapsid protein. Journal of biomedical science 2006 imex curation Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Only protein-protein interactions imex curation in vitro In vitro cosedimentation cosedimentation experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Accepted 2020-JUN-18 AT 08:05 BST AT 08:05 BST by ORCHARD Journal of biomedical science 2006 imex curation Modular organization of SARS coronavirus nucleocapsid protein. Modular organization of SARS coronavirus nucleocapsid protein. Journal of biomedical science 2006 imex curation Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Accepted 2020-JUN-18 AT 08:05 BST AT 08:05 BST by ORCHARD Journal of biomedical science 2006 imex curation ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid 43FC8750F1253034 n-61 1 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 45-365 binding region 45-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4a n-62 3 4 weight by staining molecular weight estimation by staining weight by staining experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 weight by staining molecular weight estimation by staining weight by staining experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4b n-63 2 4 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 45-365 binding region 45-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4c