IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. Development (0950-1991) 1998 imex curation Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. davet Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Accepted 2007-AUG-16 by DAVET. Development (0950-1991) 1998 davet Only protein-protein interactions imex curation The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. Development (0950-1991) 1998 imex curation Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. davet Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Accepted 2007-AUG-16 by DAVET. Development (0950-1991) 1998 davet Only protein-protein interactions imex curation The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. Development (0950-1991) 1998 imex curation Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. davet Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Papoulas O., Beek SJ., Moseley SL., McCallum CM., Sarte M., Shearn A., Tamkun JW. Chromatin - Epigenetic interactions resulting in chromatin modulation Native protein extracts were prepared from 0-12 hour embryos. Q-Sepharose anion exchange, Ni2+-chelate affinity and Superose 6 gel filtration chromatography were carried out. tamkun@biology.ucsc.edu. Accepted 2007-AUG-16 by DAVET. Development (0950-1991) 1998 davet Only protein-protein interactions imex curation q24090_drome Snr1 BAP45 Dmel\CG1064 SNR1 Snr Snr-1 l(3)01319 snr snr-1 snr1 Dmel_CG1064 CG1064 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALQTYGDKPVAFQLEEGGEYYYVGSEVGNYMRHFRGILYKKYPGMTRIVLSNEERKRLAESGLSSHILASSVSLLRAVEVDDIMAGNDEKYRAVSVNTSDTPVPRESKSKKQPQYVPTMPNSSHLDAVPQATPINRNRVHTKKVRTFPMCFDDTDPTASLENAAQKECLVPIRLDMELEGQKLRDTFTWNKNESMITPEQFAEVLCDDLDLNPLPFVPAIAQAIRQQIEAFPNDPPILEETCDQRVIVKLNIHVGNTSLVDQVEWDMSEKNNNPEEFAIKLCAELGLGGEFVTAIAYSIRGQLSWHCRTYAFSEAPLSTIDVPFRNPSDADAWAPFLETLTDAEMEKKIRDQDRNTRRMRRLANTTTGW DIP protein Q24090 original sequence version: 80 C6154768FCEA997D act1_drome Actin-5C Act5C CG4027 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P10987 original sequence version: 117 DIP protein P10987 original sequence version: 132 BDAF13C191BB1BBC q9vf03_drome Dmel_CG18740 BAP155 Dmel\CG18740 E(E2F)3B MOIRA MOR Moi Moira Mor Swi3D dmMOIRA l(3)89B1 mor CG4275 CG18740 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNTLGPKKDGSPNIDFFQSPETLQGFESIRQWLQKNCKKYLAHSSEPITKESLAQLLIHFLQYVEAKLGKNSADPPATRIPMRCFLDFKSGGGLCIIFSTMFRFRAEQRGKKFDFSIGKNPTRKDPNIQLLIEIEQALVEADLYRIPYIYIRPEIEKGFEGKLREILDNRRVEIVSDEEDATHIVYPVVDPHPDEYARPIFKRGGHVMLHWYYFPESYDSWAVNNFDLPDHIPENPESPAERWRVSASWIVDLEQYNEWMAEEDYEVDEQGKKKTHKQRMSIDDIMSFGDEKKKPAASSGGGKQKRRRSPSPASSASTSKPGKRKRSPAVVHKKSRNDDDDEDLTRDLDDPPAEPNVQEVHKANAALQSTASPAPGGKSRGDNDMMPIKGGTMTDLDDEMTGGSAAQAMSTGDGENSQTGKTSDNSNTQEFSSSAKEDMEDNVTEQTHHIIVPSYSAWFDYNSIHVIEKRAMPEFFNSKNKSKTPEIYMAYRNFMIDTYRLNPTEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQIDADVRPTPMGPPPTSHFHILSDTPSGLQSINPQKTQQPSAAKTLLDLDKKPLGKDGGLELGDKSGLTGIKTEALENGAAGGLSSGVSQFGLKLDQYAKKPAAMRNRTAASMAREWTDQETLLLLEGLEMHKDDWNKVCEHVGSRTQDECILHFLRLPIEDPYLEDDGGFLGPLACQPIPFSKSGNPIMSTVAFLASVVDPRVAAAAAKAAMEEFAAIKDEVPATIMDNHLKNVEKASAGGKFNPNFGLANSGIAGTGNDKDDEEGKEGGASASAGGSDEEMKDLSKKDDDAKSKDNTKSDKTNTNTDSSSTSSSATGNTNNTDKKPKESSGSSPSGDKSAIKSDKSNKSSPTETAAAASGGEVDIKTEDSSGDGETKDGTEAKEGTGTGTGPLAVPKEGTFSENNMQTAAAAALASAAVKAKHLAALEERKIKSLVALLVETQMKKLEIKLRHFEELEATMEREREGLEYQRQQLITERQQFHLEQLKAAEFRARQQAHHRLQQELQGQAAAGSMILPQQQQLPQAQQPQQQQQTLPPHPHLAQQQQLPPHPHQLPPQSQPLAGPTAQHQPLPPHVVSSPNGAPFAAPPISLTGGLPPGAPTAIVTNPSDQTGPVAAGAAQSQAPTPMDTTPPSSGPVSDANAPPGSAMPPGGIPPANTAPIAGVAPSS DIP protein Q9VF03 original sequence version: 85 95FEC43066F3916C q9biw6_drome Bap111 dalao CG7055 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALPSNYKQIAVGGQGSATPLQGGGGGSGGGSRSRSSGGGGGGDRNKDQTPIFTHSNYGNPAFTPQKVTKSSSSKNQNESRLPKPPKPPEKPILPYMRYSKRVWDSVKAKHPELKLWELGKKIGAMWKLLPEDEKTEFIDEYEAEKLEYEKSLKAYHQTPAYQAYMSAKSKVKTDVDMHETPSRGGGSKSQHERRIDIQPAEDEDDQDEGYTTKHLAYARYLHNHRLINEIFSEAVVPDVRSVVTTTRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDEETFQKMVLRMYEDIKRDRQRLDEPNANANSAANPAAAAATAAAAPVTRSEEAVKPPTQPGQPAATPAGQEPASAVPAPAAPPKETPPAVKPATLNPTPSSTPTPAPAVHVHETASKTDPEPMDIEPPPKPSVPPPPIKPEKLEMAAALPPQSTLVEPPKTEPAKVVAQPGKVPTPVPTPTPPPEVAPAAGAVTAATTTTRRTPTPPPVPQQVPLPQQQGGPAPPPGMPQMHPHPGHPPPGHSLMPPHMGPHQPPPGMPGLPPPPPHTGYANYGGPPHGPPPGPPGGPARPYYQPQYGGHPTPQPYYAPFSPYQQSYGPPPGSHYMSPRPPPPQHNGNPGHPYAPEHGSNPPPPQQQQQQQPPPGHLHEPSGGGPGAPGGGAGAAAAAAPGAGVYPTPGAGAGPGAPPGPAGGAPLGEAAVAGGVGPPAATALGKHPEAEKHEAD DIP protein Q9BIW6 original sequence version: 53 6717C5F3774D9790 q7k012_drome Bap55 ARP4 Arp4 BAF53 BAP47 BAP55 Baf53 Dmel\CG6546 bap55 CG6546 Dmel_CG6546 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGGTMLYGGDEIGALVFDPGHHSLRVGYAQEDSPKAEIPSVVGIGAAPETNLDPETKTDNNATPNNADQRKFYVDTNYVTVPRSNMEVQTYMKDGMIDNWDLFEKVIDYAYANVIQSEPEYHPVLFSEASWNVRNNREKLTELMFEKYNVPAFFLVKNAVLAAFSSGRATALVVDSGATHTSAVPVHEGYVLSQAVVKSPLGGDFLSRQCRQHLEKHGIDLSPVYKIASKDVVKERDNGRFTLRKLPENLTQSWQNYMLQLMMQDFQMNVLQVLENPFDERVAAQIPTVHYEFPNGYHQDFGSERFKIAESLFDNAMLGAGQLASTSVGMCDADVRLSLFGSVVVTGGNTLLQGFPERLNRDLQLRAPSNTRLKMISANGSVERRFGAWIGGSILASIGTFQQMWISSQEYEEAGKSQVERKCP DIP protein Q7K012 original sequence version: 46 7925EAB3867A6A95 bap60_drome Brahma-associated protein of 60 kDa Bap60 BRM-associated protein 60 CG4303 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSQRFAPGQAPVQSRYQPPPPAPGMRPYPPPGASFPPRGFPLHPNTTQPVPGTANVAGVPGVPGVPGVPGPSLLQRAAQQPGFQSSLRGTGAGGGGVGSGGGSKRSNESRSLGGGGSKSDFATAKKKKKLAEKILPQKVRDLVPESQAYMDLLTFERKLDATIMRKRLDIQEALKRPMKQKRKLRIFISNTFYPSKEPTNDGEEGAVASWELRVEGRLLEDGKGDPNTKIKRKFSSFFKSLVIELDKELYGPDNHLVEWHRTHTTQETDGFQVKRPGDRNVRCTILLLLDYQPLQFKLDPRLARLLGVHTQTRPVIISALWQYIKTHKLQDAHEREYINCDKYLEQIFSCQRMKFAEIPQRLNPLLHPPDPIVINHFIESGAENKQTACYDIDVEVDDTLKNQMNSFLMSTASQQEIQGLDTKIHETVDTINQMKTNREFFLSFAKDPQMFIHRWIISETRDLKLMTDVAGNPEEERRAEFYYQPWTHEAVSRYFFTKVNQKRAELEQALGIRNG DIP protein Q9VYG2 original sequence version: 71 C7823B09A2DBDF2C brm_drome ATP-dependent helicase brm Homeotic gene regulator Protein brahma brm CG5942 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD DIP protein P25439 original sequence version: 118 A4494B29F4F2E42A hsp7d_drome Heat shock 70 kDa protein cognate 4 Hsc70-4 Hsc4 CG4264 Heat shock 70 kDa protein 88E protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTYKDEKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFDLGGGTFDVSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATLDEDNLKTKISDSDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQGAGFPPGGMPGGPGGMPGAAGAAGAAGAGGAGPTIEEVD DIP protein P11147 original sequence version: 115 DIP protein P11147 original sequence version: 155 30F6BD7E07407B63 brm-bap55 3 11 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Table 1 The proteins that consistently copurify with BRM have been designated BRM associated proteins BAPs and are referred to by their molecular mass in kDa BAP45, BAP47, BAP55, BAP60, BAP74, BAP111 and BAP155. brm-snr1-1 2 4 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 5C SNR1 coimmunoprecipitates with BRM brm-snr1-2 1 10 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey physical association physical association 5B SNR1 coimmunoprecipitates with BRM