IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. EMBO J. (0261-4189) 1997 Kubota Y., Mimura S., Nishimoto S., Masuda T., Nojima H., Takisawa H. Chromatin - Epigenetic interactions resulting in chromatin modulation S-phase extract of Xenopus eggs was incubated with an equal volume of protein A beads conjugated with anti-XMCM3 davet xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kubota Y., Mimura S., Nishimoto S., Masuda T., Nojima H., Takisawa H. Chromatin - Epigenetic interactions resulting in chromatin modulation S-phase extract of Xenopus eggs was incubated with an equal volume of protein A beads conjugated with anti-XMCM3 Accepted 2008-AUG-11 by DAVET. EMBO J. (0261-4189) 1997 davet Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs. EMBO J. (0261-4189) 1997 Kubota Y., Mimura S., Nishimoto S., Masuda T., Nojima H., Takisawa H. Chromatin - Epigenetic interactions resulting in chromatin modulation S-phase extract of Xenopus eggs was incubated with an equal volume of protein A beads conjugated with anti-XMCM3 davet xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kubota Y., Mimura S., Nishimoto S., Masuda T., Nojima H., Takisawa H. Chromatin - Epigenetic interactions resulting in chromatin modulation S-phase extract of Xenopus eggs was incubated with an equal volume of protein A beads conjugated with anti-XMCM3 Accepted 2008-AUG-11 by DAVET. EMBO J. (0261-4189) 1997 davet mcm7a_xenla DNA replication licensing factor mcm7-A Minichromosome maintenance protein 7-A CDC47 homolog A CDC47p p90 mcm7-a cdc47 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MPRDYQTEKEKCKTFLQEFYKDDEIGKKHFKYGVQLANIAHREQVALYIDLDDLAEEDPELVDAICENTRRYTNLFADAVQELLPQYKEREVVHKDALDVYIEHRLMMEQRGRDPNEMRDSQNQYPPELMRRFELYFKAPSSSKARVVRDVKADSIGKLVNIRGIVTRVTEVKPMMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSRECQTNRSGGRLYLQTRGSKFIKFQELKIQEHSDQVPVGNIPRCMSVYVRGENTRLAQPGDHVGITGVFLPMLRTGFRQVVQGLLSETYLECHRLVKMNKSEDDELGTEELSEEELRQITEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDNSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVMKDPVTGEMTLEGGALVLADQGVCCIDEFDKMMDTDRTAIHEVMEQQTISIAKAGIMTTLNARCSILAAANPAYGRYNPKKTVEQNIQLPAALLSRFDVLWLIQDKPDRDNDLRLAQHITYVHQHSKQPPSQFQPLDMKLMRRYITMCKRKQPAIPEALADYLTAAYVEMRKEARTNKDMTFTSARTLLSVLRLSTALARLRLEDVVEKEDVNEAMRLMEMSKDSLLGDKGHTSRTQRPADVIFSTIREMVPEKGARSVKYSEAEQRCVSKGFTPAQFEAALEEYEELNVWLVNQARTKITFV AD623D73B501647E mcm4a_xenla DNA replication licensing factor mcm4-A mcm4-a Minichromosome maintenance protein 4-A CDC21 homolog-A P1-CDC21-A p98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSSPTSTPSRRKSKRGRGSNPPTPRGEEVQSPPSQKRRTEDSTSIGELLPMPTSPSGDIQSPLFSSPAPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRADLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVAICKEKFQRFVQRFIDPLAKEEENVGLDLNEPIYMQRLEEINVVGEPFLNIDCDHLRSFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQESFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKMFTEERVAVLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDETYDRRLAHHLVALYYQSEEQLKEEHLDMAVLKDYIAYARTYVNPRLGEEASQALIEAYVDMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSSKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPAFKYQQLFEDLRGQSDAAITKDMFDEALHALADEDYLTVTGKTVRLL 11812FE311FE5CF9 mcm6m_xenla Maternal DNA replication licensing factor mcm6 mmcm6 Maternal minichromosome maintenance protein 6 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELGGPAAAGDTDIAGQQLFKDELSDKCQKLFLEFLEECKGKDGSNLYVSAAEELIRPERNTLAVNFTDIEYYNQQLATTIQEEYYRVYPHLCRAVRSFARQMGNIPANKEFYIAFSDFPARQKIRELSSAKIGTLLRISGQVVRTHPVHPELVSGTFLCMDCQSIVKDVEQQFRYTQPTICKNPVCANRRRFTLDTNKSRFVDFQKVRIQETQAELPRGAIPRSVEIILRAEAVESAMAGDRCDFTGTLIVVPDVSALAAGDARMETGAKVTGGEGFNSEGVQGLKALGVRDLSYRLAFLACYVGATNPRFGGKDLREEDQTAESIKNQMTVQEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEIKRGVLLMLFGGVPKTTMEGTSLRGDINVCIVGDPSTSKSQFLKHVEEFSPRAVYTSGKASSAAGLTAAVVKDEESHEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPVGGRYERSKSLKHNVNLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHARNEESIERVYSIEDIQRYLLFARQFQPKITKEAEEFIVEQYRRLRQRDGSGVAKSSWRITVRQLESLIRLSESMARMHCSDEVQPKHVKEAFRLLSKSIIRVDTPDVSFDQGEDEKNIEGENNGNLNNGEEAMETNQDEPINEKPSSNAGLKMSFAEYKQISNLLVLYMQKMEETEEECHLTTTDLVNWYLKEMEAEIETETELILKKRLIEKVIHRLIYYDHILIELNKSELKTMDDTKETGEDAAEDRILVVNPNYMLED 68916B7363974731 mcm5a_xenla DNA replication licensing factor mcm5-A CDC46 homolog A CDC46p p92 mcm5-a cdc46-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFDDLGVYYSDSFGGEQQVGDDGQAKKSQLKKRFREFLRQYRIGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPTEHLQLLEEAAQEVADEVTRPRPAGEETIQEIQVMLRSDANPANIRSLKSEQMSHLVKIPGIIIAATAVRAKATKISIQCRSCRNTIGNIAVRPGLEGYAMPRKCNTEQAGRPNCPLDPYFIIPDKCKCVDFQTLKLQESPDAVPHGELPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIRKSGKTSTKGRDRVGVGIRSSYIRVVGIQVDTEGTGRSAAGAITPQEEEEFRRLAAKPDIYETVAKSIAPSIYGSSDIKKAIACLLFGGSRKRLPDGLTRRGDVNLLMLGDPGTAKSQLLKFVERCSPIGVYTSGKGSSAAGLTASVMRDPVSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVYGRWDDTKGEENIDFMPTILSRFDMIFIVKDEHNEQRDMTLAKHVMNVHLSARTQSSSVEGEVDLNTLKKYIAYCRAKCGPRLSAEAAEKLKNRYILMRSGAREHERETEKRSSIPITVRQLEAIVRISESLGKMKLQPFATETDVEEALRLFQVSTLDAAMSGSLSGVEGFTTQEDQEMLSRIEKQMKKRFAIGSQVSEHSIIQDFLKQKYPEHAIHKVLSLMMRRGEIQHRLQRKVLYRIK 702BA90C2F510720 mcm3m_xenla Maternal DNA replication licensing factor mcm3 Maternal minichromosome maintenance protein 3 P1 homolog p100 XRLF subunit beta mmcm3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDYGGGFEDHELREAQREYLDFLDDDQDQGLYHGKVRDMIGSNEHRLIVNLNDVRRKNDKRANLMLNDAFAETIAFQRALKDLVASIDATYAKQFEEFSVGFEGSFGSKHVSPRTLTASLLGSLVCVEGIVTKCSLVRPKVMRSVHYCPATKKTLERKYSDLTSLEAFPSSSIYPTKDEENNPLETEYGLSTYKDHQTLSIQEMPEKAPAGQLPRSVDIIADDDLVDKCKPGDRVQIVGIYRCLPSKQGGFTSGTFRTILLANNIKLMSKEIAPTFSADDVAKIKKFCKAHSKDIFEHLSKSLAPSIHGHEYIKKAILCMLLGGNEKVLENGTRIRGDINVLLIGDPSVAKSQLLRYVLHTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIQARLNARCSVLAAANPVYGRYDQYRTPMENIGLQDSLLSRFDLLFIVLDKMDADNDQEIADHVLRMHRYRTPGEQDGYALPLGCSVEIFATDDPNASDVTDQELQIYEKHDNLLHGPRKNKSKIVSMQFIRKYIHVAKLIKPVLTSEAADYISQEYAKIRNHDQINNDSARTMPVTARALETMIRLSTAHAKVRMSKTIERQDAETALELVQFAYFKKVLAKEKKKTDKDLHDENLSQDTLSQESVRKSSRRAGKIADSQDDSMDPYSFSEQDSSLNENLSQSLRPQRKKAESQDGKRSLSQNRTKEFKAALLKAFKSSRSQSVAVSQLLELINKGNPEPFERSEVKEALDNMQNDNQVMVSEDVVFLI 70B08E9BE0F3A81F mcm2_xenla DNA replication licensing factor mcm2 Minichromosome maintenance protein 2 BM28-homolog p112 mcm2 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MADSSESFNIATSPRAGSRRDALTSSPGRDLPPFEDESEGMFGDGVVPEEEEDGEELIGDAMERDYRPISELDRYEVEGLDDEEDVEDLTASQREAAEQSMRMRDREMGRELGRMRRGLLYDSDEEEEDRPARKRRMAERAAEGAPEEDEEMIESIENLEDMKGHTVREWVSMAATRLEIYHRFKNFLRTHVDEHGHNVFKEKISDMCKENKESLPVNYEDLAAREHVLAYFLPEAPAEMLKIFDEAAKEVVLVMYPKYDRIAREIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTCCTGVLPQLSMVKYNCNKCNFILGPFFQSQNQEVRPGSCPECQSFGPFEINMEETVYQNYQRITIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHITKKDDKVAVGELTDEDVKAIVALSKDERIGERIFASIAPSIYGHEDIKRGLALALFGGEAKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYVEKVASRAVFTTGQGASAVGLTAYVQRHPVTKEWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAASNPIGGRYDPSLTFSENVDLTEPIVSRFDILCVVRDTVDPVQDEMLARFVVSSHIKHHPSSKDIANGDAAEFALPNTFGVEALPQEVLKKYIMYAKEKIRPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARMHLRDYVVEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLAFRRDNNELLLFVLKQLIAEQVTYQRNRYGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSDLFKMNKFTHDVKKKLIIQQF 524C7B3673E2B2DD xmcm3-mcm6m 1 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association These results indicate that six immunologically distinct proteins; p112, p102, XMCM3, p98, p92 and p90, are present in the immunoprecipitates. Table 1 and Fig 1 xmcm3-mcm6m-1 2 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association These results indicate that six immunologically distinct proteins; p112, p102, XMCM3, p98, p92 and p90, are present in the immunoprecipitates. 6 These are immunodepletion analyssi and the absence of protein indicates its presence in the complex.