IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Mech. Dev. (0925-4773) 2003 Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation 0-16 hr embryonic cDNA library used yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). 0-16 hr embryonic cDNA library used Mech. Dev. (0925-4773) 2003 Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Mech. Dev. (0925-4773) 2003 Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation 0-16 hr embryonic cDNA library used in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation 0-16 hr embryonic extract was used Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). 0-16 hr embryonic cDNA library used Mech. Dev. (0925-4773) 2003 Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. Mech. Dev. (0925-4773) 2003 Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation 0-16 hr embryonic cDNA library used drome Drosophila melanogaster (Fruit fly) Fruit fly coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Mishra K., Chopra VS., Srinivasan A., Mishra RK. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). 0-16 hr embryonic cDNA library used Mech. Dev. (0925-4773) 2003 php_drome Polyhomeotic-proximal chromatin protein CG18412 ph-p protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDRRALKFMQKRADTESDTTTPVSTTASQGISASAILAGGTLPLKDNSNIREKPLHHNYNHNNNNSSQHSHSHQQQQQQQVGGKQLERPLKCLETLAQKAGITFDEKYDVASPPHPGIAQQQATSGTGPATGSGSVTPTSHRHGTPPTGRRQTHTPSTPNRPSAPSTPNTNCNSIARHTSLTLEKAQNPGQQVAATTTVPLQISPEQLQQFYASNPYAIQVKQEFPTHTTSGSGTELKHATNIMEVQQQLQLQQQLSEANGGGAASAGAGGAASPANSQQSQQQQHSTAISTMSPMQLAAATGGVGGDWTQGRTVQLMQPSTSFLYPQMIVSGNLLHPGGLGQQPIQVITAGKPFQGNGPQMLTTTTQNAKQMIGGQAGFAGGNYATCIPTNHNQSPQTVLFSPMNVISPQQQQNLLQSMAAAAQQQQLTQQQQQFNQQQQQQLTQQQQQLTAALAKVGVDAQGKLAQKVVQKVTTTSSAVQAATGPGSTGSTQTQQVQQVQQQQQQTTQTTQQCVQVSQSTLPVGVGGQSVQTAQLLNAGQAQQMQIPWFLQNAAGLQPFGPNQIILRNQPDGTQGMFIQQQPATQTLQTQQNQIIQCNVTQTPTKARTQLDALAPKQQQQQQQVGTTNQTQQQQLAVATAQLQQQQQQLTAAALQRPGAPVMPHNGTQVRPASSVSTQTAQNQSLLKAKMRNKQQPVRPALATLKTEIGQVAGQNKVVGHLTTVQQQQQATNLQQVVNAAGNKMVVMSTTGTPITLQNGQTLHAATAAGVDKQQQQLQLFQKQQILQQQQMLQQQIAAIQMQQQQAAVQAQQQQQQQVSQQQQVNAQQQQAVAQQQQAVAQAQQQQREQQQQVAQAQAQHQQALANATQQILQVAPNQFITSHQQQQQQQLHNQLIQQQLQQQAQAQVQAQVQAQAQQQQQQREQQQNIIQQIVVQQSGATSQQTSQQQQHHQSGQLQLSSVPFSVSSSTTPAGIATSSALQAALSASGAIFQTAKPGTCSSSSPTSSVVTITNQSSTPLVTSSTVASIQQAQTQSAQVHQHQQLISATIAGGTQQQPQGPPSLTPTTNPILAMTSMMNATVGHLSTAPPVTVSVTSTAVTSSPGQLVLLSTASSGGGGSIPATPTKETPSKGPTATLVPIGSPKTPVSGKDTCTTPKSSTPATVSASVEASSSTGEALSNGDASDRSSTPSKGATTPTSKQSNAAVQPPSSTTPNSVSGKEEPKLATCGSLTSATSTSTTTTITNGIGVARTTASTAVSTASTTTTSSGTFITSCTSTTTTTTSSISNGSKDLPKAMIKPNVLTHVIDGFIIQEANEPFPVTRQRYADKDVSDEPPKKKATMQEDIKLSGIASAPGSDMVACEQCGKMEHKAKLKRKRYCSPGCSRQAKNGIGGVGSGETNGLGTGGIVGVDAMALVDRLDEAMAEEKMQTEATPKLSESFPILGASTEVPPMSLPVQAAISAPSPLAMPLGSPLSVALPTLAPLSVVTSGAAPKSSEVNGTDRPPISSWSVDDVSNFIRELPGCQDYVDDFIQQEIDGQALLLLKEKHLVNAMGMKLGPALKIVAKVESIKEVPPPGEAKDPGAQ CF9B543DDBC79FBC asx_drome Polycomb protein Asx Asx CG8787 Additional sex combs protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKTITPDTTTTTSSQHQQLLIPQADQHHQPMLQQQSLLAAPPPTMIMEHVNLVDDDEKDPLALEQLEVSPSTKHTHSLRRHLPRIIVKPIPPEKKPMAPSEEAAVSTAPAPPTRLICSRRIQQQQQVKAAAAAAAAAAAAAAAAAAAAQAQATSSYPSAISPGSKAGTSQASTMREVLASIPGFSVKPRRRSNKKLTTAAQIEQTKDGKIDLETPDSILASTNLRALLNKQTFSLLPPLYQYNLIQLLPSVDREASELEQPSSSASGGSPSEAIRLSASCLNNEFFARACLEWRERLSEGEFTPENQLKLKTEAEREKNKLDPWKLKHFEPFWGEKNSRGKDKDKLESDCKNQKLSASIKSEPKPPATSQQKPLQQATCDNETELKFDLSTKCETTSAKTTVAVAVADKSSTFPPTGSQNNVLNEQQRRVLKRPSSSPSQRKQAPTTIATINLDDDLDELPSTSKDSKQPKMDEIVPNASGNVVAAPMVDVVDHSAVEMKIKDEQQHQRQHQPLINSTCDKIEPSECSKEMIVAMKQVDSKEDVDSIASAAAMPAIAAVTPHTPKPEALAPNPDVANQFVSYLQNVELAAETKAPLDNSNEADITTGTNSHDFVFSDTIDHAYFQEHQSTINHNFFTSSSSSNTATTAANKLEEHSDKPEDSPLPIASSISGSTPASSITSTSCTSSSSSSASMSSSCSSSNSGSTTTAPTTSSSAGAPTAPLTLAAAAETTLANVQAMLSTVAKLQQQQQELPVELNSNEMYQHVQHDWNFGDIKLSSSQSSGDQQRNLSHEAIDLMDVVQDADVIDDIMHNDVCHDVLGDEDEGDQEEDEDDEVVECMTEEQQLIDEDSEAVREIVDKLQQHQQQQNQQQHHQQLHIQDVVQLAQHSFMPQAHSEFGNDIGQEMLCDAVPMSAAEMEVSSTVITNSSNSNDSSNNISLCSSTNSLTINQMPHQASQQPQQNAQSNAQQQRQILVDSNGQIIGNFLLQQQRQQQQQQLLQQFTLQAAAAQQQQQQQQQHQQQQQQQQQATSSNSLGKTLPVALRNGTQQFLSPNLIAQQHQQQQQQQLEQHQQQATAQQKHQQIQQFALQQAQLHQRQLLAQAANNNLLQQQQQQQQNVALPTTQAKFIAKPLNIISMTRPANASPTTAATTANTASIPSAYANVVAVTGAQQQQSPPVPAPQQQTVQQQQLANHNSNMQQLPNVLTMKTLPPSGVPTTIAQQRLQPKMPTGKGRKATSNRLPPGAVNLERSYQICQAVIQNSPNRENLKAQLRPPAAILNQHQPTTTTAPAPINPVTLNVSTVAATPMSNITTATGSMAAAVAAAPPQNVLKQEELLVSGAVGAGALPAGLPPNVMGVGRPGVYKVIGPRMSGFPRKKYVQRKPSPTTLIRHVFSPGPGGATATAQQLQMLQQHHQSTTSPVPVQNPQQPAPEQLIHQNGNGQYVLVHRANVGAADNQAPRASSAPPMHQNQFVTVQNPLHSINGIPMGGRGRPASVDTTAGSGNVIAPPISATDALHHHHHEMQQQQQHQQPQPLGNVGAAANIVRRNIAAGPNIAYIDGSNTNSSAVALMEAGNNYIVTTNASPTAAPSPINQQPQSQPTGTQHQHPLLQLHQTGENTPPGNEATATANNCACSLNAMVICQQCGAFCHDDCIGAAKLCVACVIR DIP protein Q9V727 original sequence version: 73 F65D87398D67D321 lolal_drome Longitudinals lacking protein-like lolal ban Protein Batman CG5738 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMSSDQQFFLKWNDFQTNMVTSFRHLRDEKSFTDVTLACEGQTCKAHKMVLSACSPYFKALLEENPSKHPIIILKDVSYIHLQAILEFMYAGEVNVSQEQLPAFLKTADRLKVKGLAETPSSIKREG 3D5DE2C4E2E06B2D gaga_drome Transcription factor GAGA Trl GAGA TFGAGA Trithorax-like protein GAGA factor Adh transcription factor 2 Neural conserved at 70F Adf-2 Nc70F CG33261 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKTTSGKKSSSGSSGSGSGALSSSGSVPQVQTVQSLHTLQGVQVKKDPDAQQQQQQQQQQQQQQQQAMTVSGATGGQVQQQVQQVQQQVQQQQQQQQQQQQQLQHHQIIDSSGNITTATTSAQAAAAAQQQAAGQQQQLVAQSDGSESGAPLSIAQVQTLQGHQIIGNLNQVNMTDFQQQQPQQQQQQQQQQQQQQQQQQQTQQTL 04826A1B630E3320 trl-lolal-2 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association AA 1-245 of GAGA was used as bait. A embryonic cDNA libary was used as prey. 50000 transformants were screened from this a total of 23 positives were identified 14 of these contained the lolal gene. Revisit when Protein Editor in place trl-lolal-3 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey association association GST-LOLAL was expressed in E. coli. GAGA was from nuclear extract. trl-ph_p-1 3 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association The interaction was dependent on external added GST-lolal in embryonic extract. However, in larval extract it was indenpendent of GST-lolal.