UniProt UniProt http://www.uniprot.org Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Mol. Cell. Biol. (0270-7306) 2002 imex curation Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu Accepted 2006-AUG-29 by JYOTI. 13-SEP-2006: Contacted by JYOTI Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Mol. Cell. Biol. (0270-7306) 2002 imex curation Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu Accepted 2006-AUG-29 by JYOTI. 13-SEP-2006: Contacted by JYOTI Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex. Mol. Cell. Biol. (0270-7306) 2002 imex curation Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Howe L., Kusch T., Muster N., Chaterji R., Yates JR., Workman JL. Chromatin - Epigenetic interactions resulting in chromatin modulation Paper shows coip of H3 HAT activity with FLAG-YNG1 and SAS3, fig. 2. Complex association not clear therefore not annotated. Yeast whole cell extract was used to purify NuA3 complex from a FLAG-epitope-tagged Sas3p strain. jlw10@psu.edu Accepted 2006-AUG-29 by JYOTI. 13-SEP-2006: Contacted by JYOTI Mol. Cell. Biol. (0270-7306) 2002 Only protein-protein interactions imex curation spt16_yeast FACT complex subunit SPT16 SPT16 CDC68 SSF1 YGL207W Facilitates chromatin transcription complex subunit SPT16 Suppressor of Ty protein 16 Cell division control protein 68 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENPYQKTTILHNWLLSYEFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLELWQRNNKEPELNKKLFDDVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEFNVIDISLGLSKVWEVKDVNEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLSDKIENKIDDVKFLKQLSPDLSALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARSTNDQLYGNGCILASCGIRYNNYCSNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKPGRTPKEVYESVIEYIEKTKPELVPNFTKNIGSLIGLEFRDSNFILNVKNDYRKIQRGDCFNISFGFNNLKDSQSANNYALQLADTVQIPLDETEPPRFLTNYTKAKSQISFYFNNEEEDNNKKKSSPATKVPSKPDRNSKILRTKLRGEARGGAEDAQKEQIRKENQKKLHEKLEKNGLLRFSAADANGPDSEPRQYFKKYESYVRDSQLPTNIRDLRIHVDWKSQTIILPIYGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPGSSGGISKKVEELPYEESADNQFVRSITLRSKDGDRMSETFKQIADLKKEATKREQERKALADVVQQDKLIENKTGRTKRLDQIFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDILFSNIKNLIFQSCKGELIVVIHIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQSRFRRYGDEDELEQEQEERRKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNRSAVFCMPTTDCLVQLIEPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWLTDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDEASDESEEEVSEYEASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDELEKKAARADRGANFRD 4F01C772E299E2E6 4F01C772E299E2E6 sas3_yeast Histone acetyltransferase SAS3 SAS3 YBL052C YBL0515 YBL0507 Something about silencing protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTANDESPKPKKNALLKNLEIDDLIHSQFVRSDTNGHRTTRRLFNSDASISHRIRGSVRSDKGLNKIKKGLISQQSKLASENSSQNIVNRDNKMGAVSFPIIEPNIEVSEELKVRIKYDSIKFFNFERLISKSSVIAPLVNKNITSSGPLIGFQRRVNRLKQTWDLATENMEYPYSSDNTPFRDNDSWQWYVPYGGTIKKMKDFSTKRTLPTWEDKIKFLTFLENSKSATYINGNVSLCNHNETDQENEDRKKRKGKVPRIKNKVWFSQIEYIVLRNYEIKPWYTSPFPEHINQNKMVFICEFCLKYMTSRYTFYRHQLKCLTFKPPGNEIYRDGKLSVWEIDGRENVLYCQNLCLLAKCFINSKTLYYDVEPFIFYILTEREDTENHPYQNAAKFHFVGYFSKEKFNSNDYNLSCILTLPIYQRKGYGQFLMEFSYLLSRKESKFGTPEKPLSDLGLLTYRTFWKIKCAEVLLKLRDSARRRSNNKNEDTFQQVSLNDIAKLTGMIPTDVVFGLEQLQVLYRHKTRSLSSLDDFNYIIKIDSWNRIENIYKTWSSKNYPRVKYDKLLWEPIILGPSFGINGMMNLEPTALADEALTNETMAPVISNNTHIENYNNSRAHNKRRRRRRRSSEHKTSKLHVNNIIEPEVPATDFFEDTVSSLTEYMCDYKNTNNDRLIYQAEKRVLESIHDRKGIPRSKFSTETHWELCFTIKNSETPLGNHAARRNDTGISSLEQDEVENDVDTELYVGENAKEDEDEDEDFTLDDDIEDEQISEENDEEEDTYEEDSDDDEDGKRKGQEQDENDIESHIRKERVRKRRKITLIEDDEE ACF5B1B225CB4A71 yng1_yeast Protein YNG1 ING1 homolog 1 YNG1 YOR29-15 YOR064C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEHLANENSDSDIRYSFLSTLDHLPCELIRSLRLMQTIDLFKNEEDEPGMERACRDLLLVATYINDLVDDQIHFLKQHKKELEIQKSVTKNFNSSLENIKSKLTLEEPGAYKEPKLLLKINLKKAKSRERKESITSPTIGINQGDVTEGNNNQEEVYCFCRNVSYGPMVACDNPACPFEWFHYGCVGLKQAPKGKWYCSKDCKEIANQRSKSKRQKRRK 5232A71839E2FDA4 5232A71839E2FDA4 taf14_yeast Transcription initiation factor TFIID subunit 14 ANC1 CST10 YPL129W TAF14 SWP29 TAF30 TFG3 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex subunit TAF14 SWI/SNF complex 29 kDa subunit Actin non-complementing mutant 1 Chromosome stability protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 60EA5EC2474B1B9C 60EA5EC2474B1B9C sas3-taf14-yng1-1 3 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1A, B and C yng1-sas3-1 1 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2B taf14-sas3-1 1 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3A Interaction is independent on presence of YNG1. taf14-yng1-1 1 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 B Interaction is dependent on the presenece of SAS3. spt16-sas3-1 2 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait physical association physical association 5A Interaction is not dependent on presence of YNG1.