IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu A mouse E9.5 and E10.5 cDNA library in the vector pVP16 and a mouse newborn brain cDNA phage library were used. Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation In-vivo expression of full-length FLAG-tagged-Sds3 along with an N-terminal fragment (AA 1-187) of Sds3 in 293T cells followed by immunoprecipitation with anti-FLAG antibody results in coimmunoprecipitation of Sds3 N-terminal fragment detected by Western Blot using anti-Sds3 antibody. Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Various fragments of the Sds3 were used as bait alongside full-length Sds3 prey to determine the self-interacting domain of Sds3. Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu in vitro In vitro pull down pull down predetermined predetermined participant predetermined Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Bacterially expressed GST full-length Sds3 fusion protein was tested for its ability to interact with radiolabeled in-vitro translated Sin3a, sin3b or other components of the Sin3 repressor complex. Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu mouse Mus musculus Mouse coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip polyclonal western polyclonal antibody western blot polyclonal western Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Tissue extracts derived from various organs of newborn mice were immunoprecipitated with anti-Sds3 antibody. Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu in vitro In vitro pull down pull down predetermined predetermined participant predetermined Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex. Mol. Cell. Biol. (0270-7306) 2002 Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Fig 2a ron_depinho@dfci.harvard.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Alland L., David G., Shen-Li H., Potes J., Muhle R., Lee HC., Hou H., Chen K., DePinho RA. Chromatin - Epigenetic interactions resulting in chromatin modulation Various fragments of the Sds3 prey were used alongside full-length Sin3b bait to determine the Sin3-interacting domain of Sds3. Fig 2a ron_depinho@dfci.harvard.edu Mol. Cell. Biol. (0270-7306) 2002 sin3a_mouse Paired amphipathic helix protein Sin3a Sin3a Kiaa4126 Transcriptional corepressor Sin3a Histone deacetylase complex subunit Sin3a protein protein mouse Mus musculus Mouse MKRRLDDQESPVYAAQQRRIPGSTEAFSHQHRVLAPAPPVYEAVSETMQSATGIQYSVAPNYQVSAVPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQPQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQIPTHGIQPQPQPPPQHPSQPSSQSAPTPAQPAPQPTAAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPVQPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYTPALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRPSQNGCQIRRHSGTGATPPVKKKPKLMSLKESSMADASKHGVGTESLFFDKVRKALRSAEAYENFLRCLVIFNQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLESFPKERATEGIAMEIDYASCKRLGSSYRALPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLATIRVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRLKMKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENAGVPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEEEEMDVDEATGAPKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRICSQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQYEDSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEVCVQVTDLYLAENNNGATGGQLNSQTSRSLLESAYQRKAEQLMSDENCFKLMFIQSQGQVQLTVELLDTEEENSDDPVEAERWSDYVERYMSSDTTSPELREHLAQKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVESLDKLECRFKLNSYKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP DIP protein Q60520 original sequence version: 144 DIP protein Q60520 original sequence version: 106 DIP protein Q60520 original sequence version: 115 DIP protein Q60520 original sequence version: 158 80FE378F62ED7CB3 sds3_mouse Sin3 histone deacetylase corepressor complex component SDS3 Suppressor of defective silencing 3 protein homolog Suds3 Sds3 protein protein mouse Mus musculus Mouse MSAAGLLAPAPAPAAAPAAPEYYPEDEEELESAEDDERSCRGRESDEDTEDASETDLAKHDEEDYVEMKEQMYQDKLASLKRQLQQLQEGTLQEYQKRMKKLDQQYRERIRNAELFLQLETEQVERNYIKEKKAAVKEFEDKKVELKENLIAELEEKKKMIENEKLTMELTGDSMEVKPIMTRKLRRRPNDPVPIPDKRRKPAPAQLNYLLTDEQIMEDLRTLNKLKSPKRPASPSSPEHLPATPAESPAQRFEARIEDGKLYYDKRWYHKSQAIYLESKDNQKLSCVISSVGANEIWVRKTSDSTKMRIYVGQLQRGLFVIRRRSAA 05C22EA578C318E8 sin3b_mouse Paired amphipathic helix protein Sin3b Transcriptional corepressor Sin3b Histone deacetylase complex subunit Sin3b Sin3b Kiaa0700 protein protein mouse Mus musculus Mouse MAHAGSGGSAGRGFGGSRWGRSGSGGHEKLPVHVEDALTYLDQVKIRFGSDPATYNGFLEIMKEFKSQSIDTPGVIRRVSQLFHEHPDLIVGFNAFLPLGYRIDIPKNGKLNIQSPLSSQDNSHSHGDCGEDFKQMSYKEDRGQVPLESDSVEFNNAISYVNKIKTRFLDHPEIYRSFLEILHTYQKEQLHTKGRPFRGMSEEEVFTEVANLFRGQEDLLSEFGQFLPEAKRSLFTGNGSCEMNSGQKNEEKSLEHNKKRSRPSLLRPVSAPAKKKMKLRGTKDLSIAAVGKYGTLQEFSFFDKVRRVLKSQEVYENFLRCIALFNQELVSGSELLQLVSPFLGKFPELFAQFKSFLGVKELSFAPPMSDRSGDGISREIDYASCKRIGSSYRALPKTYQQPKCSGRTAICKEVLNDTWVSFPSWSEDSTFVSSKKTPYEEQLHRCEDERFELDVVLETNLATIRVLESVQKKLSRMAPEDQEKLRLDDCLGGTSEVIQRRAIHRIYGDKAPEVIESLKRNPATAVPVVLKRLKAKEEEWREAQQGFNKIWREQYEKAYLKSLDHQAVNFKQNDTKALRSKSLLNEIESVYDEHQEQHSEGRSAPSSEPHLIFVYEDRQILEDAAALISYYVKRQPAIQKEDQGTIRQLLHRFLPSLFFSQQCPGTSDDSADERDRDRDSAEPERRRPTDEKPPADASPEPPKVLDDVYSLFFANNNWYFFLRLHQTLCARLLKIYRQAQKQLLEHRREQEREQLLCEGRREKAADPAMELRLKQPSEVELEEYYPAFLDMVRSLLEGSIDPTQYEDTLREMFTIHAYIGFTMDKLVQNIARQLHHLVSDDVCLKVVELYLNEQQRGAAGGNLSSRCVRAARETSYQWKAERCMADENCFKVMFLQRRGQVIMTIELLDTEEAQTEDPVEVQHLARYVEQYVGSEGASSSSTEGFLLKPVFLQRNLKKFRRWQCEQVRAMRGEAKSSWKRLMGVESACDVDCRFRLGTHKMVFIVNSEDYMYRRGTLCRAKQVQPLVLLRHHRHFEEWHGRWLEDNVTVAAAGLVQDWLMGEEEEDMVPCKTLCETAHVHGLPVTRYRVQYSRRPASP A73F6DFC11EBA256 sds3-sin3a-1 7 12 unspecified role unspecified role prey prey vp16-tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 11 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association sds3-sin3b-1 7 12 unspecified role unspecified role prey prey vp16-tad tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association The full-length Sds3 protein was able to interact with a short region of Sin3b (AA 383-550). sds3-sin3b-2 10 12 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain vp16-tad tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association The full-length Sin3b protein was able to interact with a short region of Sds3 (AA 43-229). By deletion analysis it was found that a region AA 188-226 in Sds3 played an essential role in associating with the full-length Sin3b but was not sufficient for interaction. sds3-sin3a-2 5 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association sds3-sin3b-3 5 13 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) physical association physical association sds3-sin3b-4 2 12 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Displays that interaction between Sds3 and Sin3b occurs in-vivo as well as in-vitro. Deletion of AA 383-550 in Sin3b abolished interaction with Sds3 and other components of the Sin3-complex. Also deletion of AA 188-226 in Sds3 had a similar affect. Both Sds3 ans Sin3b are overexpressed. sds3-sds3-1 4 12 unspecified role unspecified role prey prey vp16-tad tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) physical association physical association The full-length Sds3 protein was able to interact with a short region of Sds3 (AA 72-108) for homodimerization. sds3-sds3-2 3 12 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association The full-length Sds3 protein was able to interact with a short region of Sds3 (AA 1-187) for homodimerization in-vivo. Both the full-length and the n-terminus Sds3 are overexpressed. sds3-sds3-3 8 12 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association The full-length GST-tagged-Sds3 protein was able to interact with in-vitro translated full-length Sds3 indicating that homodimerization seems to occurs via direct protein-protein interaction. sds3-sin3a-3 1 12 unspecified role unspecified role prey prey in vitro In vitro 11 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association The full-length Sds3 protein was isolated using a c-terminus region of Sin3A (AA 534-1274). sds3-sin3a-4 6 11 unspecified role unspecified role bait bait mouse Mus musculus Mouse 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association Both proteins are endogenous. sds3-sin3b-5 9 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Displays that interaction between Sds3 and Sin3b occurs in-vivo as well as in-vitro. Deletion of AA 383-550 in Sin3b abolished interaction with Sds3 and other components of the Sin3-complex. Also deletion of AA 188-226 in Sds3 had a similar affect. Both Sds3 ans Sin3b are overexpressed.