UniProt UniProt http://www.uniprot.org BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J. Cell Biol. (0021-9525) 2001 imex curation Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R., Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu 16-Oct-2008: Contacted by JYOTI. human ovary cDNA davet Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu Accepted 2008-OCT-15 by DAVET. 16-Oct-2008: Contacted by JYOTI. human ovary cDNA J. Cell Biol. (0021-9525) 2001 davet mimix curation IMEx BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J. Cell Biol. (0021-9525) 2001 imex curation Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R., Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu 16-Oct-2008: Contacted by JYOTI. human ovary cDNA davet Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu Accepted 2008-OCT-15 by DAVET. 16-Oct-2008: Contacted by JYOTI. human ovary cDNA J. Cell Biol. (0021-9525) 2001 davet mimix curation IMEx BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J. Cell Biol. (0021-9525) 2001 imex curation Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R., Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu 16-Oct-2008: Contacted by JYOTI. human ovary cDNA davet Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu Accepted 2008-OCT-15 by DAVET. 16-Oct-2008: Contacted by JYOTI. human ovary cDNA J. Cell Biol. (0021-9525) 2001 davet mimix curation IMEx BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J. Cell Biol. (0021-9525) 2001 imex curation Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R., Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu 16-Oct-2008: Contacted by JYOTI. human ovary cDNA davet Only protein-protein interactions imex curation crigr-cho_ao3_1 Cricetulus griseus cho_ao3_1 Chinese hamster AO3_1 cell line confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu Accepted 2008-OCT-15 by DAVET. 16-Oct-2008: Contacted by JYOTI. human ovary cDNA J. Cell Biol. (0021-9525) 2001 davet mimix curation IMEx BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. J. Cell Biol. (0021-9525) 2001 imex curation Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R., Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu 16-Oct-2008: Contacted by JYOTI. human ovary cDNA davet Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ye Q., Hu YF., Zhong H., Nye AC., Belmont AS., Li R. Chromatin - Epigenetic interactions resulting in chromatin modulation HEK293T cells have been transfected with FLAG-tagged COBRA1. rl2t@virginia.edu Accepted 2008-OCT-15 by DAVET. 16-Oct-2008: Contacted by JYOTI. human ovary cDNA J. Cell Biol. (0021-9525) 2001 davet mimix curation IMEx brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF ctip_human DNA endonuclease RBBP8 RBBP8 CTIP CtBP-interacting protein Retinoblastoma-binding protein 8 Retinoblastoma-interacting protein and myosin-like Sporulation in the absence of SPO11 protein 2 homolog protein protein human Homo sapiens Human MNISGSSCGSPNSADTSSDFKDLWTKLKECHDREVQGLQVKVTKLKQERILDAQRLEEFFTKNQQLREQQKVLHETIKVLEDRLRAGLCDRCAVTEEHMRKKQQEFENIRQQNLKLITELMNERNTLQEENKKLSEQLQQKIENDQQHQAAELECEEDVIPDSPITAFSFSGVNRLRRKENPHVRYIEQTHTKLEHSVCANEMRKVSKSSTHPQHNPNENEILVADTYDQSQSPMAKAHGTSSYTPDKSSFNLATVVAETLGLGVQEESETQGPMSPLGDELYHCLEGNHKKQPFEESTRNTEDSLRFSDSTSKTPPQEELPTRVSSPVFGATSSIKSGLDLNTSLSPSLLQPGKKKHLKTLPFSNTCISRLEKTRSKSEDSALFTHHSLGSEVNKIIIQSSNKQILINKNISESLGEQNRTEYGKDSNTDKHLEPLKSLGGRTSKRKKTEEESEHEVSCPQASFDKENAFPFPMDNQFSMNGDCVMDKPLDLSDRFSAIQRQEKSQGSETSKNKFRQVTLYEALKTIPKGFSSSRKASDGNCTLPKDSPGEPCSQECIILQPLNKCSPDNKPSLQIKEENAVFKIPLRPRESLETENVLDDIKSAGSHEPIKIQTRSDHGGCELASVLQLNPCRTGKIKSLQNNQDVSFENIQWSIDPGADLSQYKMDVTVIDTKDGSQSKLGGETVDMDCTLVSETVLLKMKKQEQKGEKSSNEERKMNDSLEDMFDRTTHEEYESCLADSFSQAADEEEELSTATKKLHTHGDKQDKVKQKAFVEPYFKGDERETSLQNFPHIEVVRKKEERRKLLGHTCKECEIYYADMPAEEREKKLASCSRHRFRYIPPNTPENFWEVGFPSTQTCMERGYIKEDLDPCPRPKRRQPYNAIFSPKGKEQKT DIP protein Q99708 original sequence version: 111 DIP protein Q99708 original sequence version: 92 DIP protein Q99708-1 original sequence version: 138 DIP protein Q99708 original sequence version: 150 DIP protein Q99708 original sequence version: 110 DIP protein Q99708-1 original sequence version: 110 E028DE56DE55C0F2 nelfb_human Negative elongation factor B NELFB COBRA1 Cofactor of BRCA1 KIAA1182 protein protein human Homo sapiens Human MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL DIP protein Q8WX92 original sequence version: 82 C0A4F7F2BEE52CBD brca1-cobra-1 5 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association not shown cobra1-brca1-2 1 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey physical association physical association 5A cobra1-brca1-1 3 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P38398:p.Ala1708Glu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain A E Wed May 02 15:17:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala1708glu' Tue Jun 30 19:35:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1708Glu' P38398:p.Met1775Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain M R Wed May 02 15:30:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met1775arg' Tue Jun 30 15:39:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met1775Arg' region lac repressor-BRCA1 fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 5B brca1-cobra1-1 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P38398:p.Met1775Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain M R Wed May 02 19:16:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met1775arg' Tue Jun 30 17:46:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met1775Arg' ecoli Escherichia coli (strain K12) physical association physical association 5c brca1-rbbp8-3 2 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P38398:p.Met1775Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain M R Wed May 02 15:35:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met1775arg' Tue Jun 30 17:05:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met1775Arg' ecoli Escherichia coli (strain K12) physical association physical association 5c cobra1-brca1--1 4 6 unspecified role unspecified role neutral component neutral component region lac repressor-BRCA1 fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_ao3_1 Cricetulus griseus cho_ao3_1 Chinese hamster AO3_1 cell line 8 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_ao3_1 Cricetulus griseus cho_ao3_1 Chinese hamster AO3_1 cell line colocalization colocalization 6