IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. Human heart cDNA library. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 confocal microscopy confocal microscopy immunostaining immunostaining Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. GFP tagged molecules were detected by tag fluorescence. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions mouse-heart Mus musculus heart [Cardiac; Cardiac tissue] heart Heart [Cardiac; Cardiac tissue] confocal microscopy confocal microscopy immunostaining immunostaining Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. Heart tissue from muscle LIM protein (MLP) knockout mice, a model for dilated cardiomyopathy. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions rat-cardiac_myocytes_neo rat cardiac myocytes neo neonatal_ventricular_myocyte neonatal ventricular myocyte confocal microscopy confocal microscopy immunostaining immunostaining Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. Neonatal rat cardiomyocytes. GFP tagged molecules were detected by tag fluorescence. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J. Cell Biol. (0021-9525) 2010 imex curation Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. q2v2m9-4:- UniProt were contacted about integrating this new isoform into main entry Q2V2M9. [existing entry (Trembl E5F5Q0) for FHOD3 - with TDEEEEVE sequence + more...] SO says make the Isoform-4 as an Internal protein,leave a note to this effect, and then make sure it maps! The new isoform should be visible in UniProt in the January 2013 release, Problem with amino acid numbering for FH2L domain - not given in the paper -see addition to Data-Proc comment. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk 18-Dec-2012: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Iskratsch T., Lange S., Dwyer J., Kho A.L., dos Remedios C., Ehler E. Cardiac - Interactions involving cardiac related proteins Fig 1D Kinase assay not added as there is no purification I can see. p62/SQSTM1 has been entered as Human based on a sequence length of 440 aas as shown in Figure 3. The amino acid numbering for the FH2(L) and FH2(S) domains of FHOD3 were given by the authors. Elisabeth.ehler@kcl.ac.uk Accepted 2012-DEC-14 by ORCHARD 18-Dec-2012: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2010 Only protein-protein interactions imex curation q5d003_rat protein protein rat Rattus norvegicus (Rat) Rat LSVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKIAILNFDEYALNKEGIEKILTMIPTEEEKQKIQEAQLANPEVPLGSAEQFLLTLSSISELSARLHLWAFKMDYETTEKEVAEPLLDLKEGIDQLENNKTLGFILSTLLAIGNFLNGTNAKAFELSYLEKVPEVKDTVHKQSLLHHVCTMVVENFPDSSDLYSEIGAITRSAKVDFDQLQDNLCQMERRCKASWDHLKAIAKHEMKPVLKQRMSELLKDCAERIIILKIVHRRIINRFHSFLLFMGHPPYAIREVNINKFCRIISEFALEYRTTRERVLQQKQKRANHRERNKTRGKMITDSGKFSGSSPAAPSQPQGLSYAEDAAEHENMKAVLKTSSPALEDATPMLGVCTRSRASRGSTSSWTMGTEESPSVPDDAADEIMDRIVKSATQVPSQRVVPRERKRSRANRKSLRRTLKSGLTPEEARALGLVGTSELQL 074ACB9E11B1AC8C sqstm_mouse Sequestosome-1 Ubiquitin-binding protein p62 STONE14 Sqstm1 A170 STAP protein protein mouse Mus musculus Mouse MASFTVKAYLLGKEEATREIRRFSFCFSPEPEAEAQAAAGPGPCERLLSRVAVLFPTLRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPPEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTTPESSSTGTEDKSNTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q64337 original sequence version: 121 DIP protein Q64337 original sequence version: 130 ED4CCCA7741D35CA csk21_human Casein kinase II subunit alpha CSNK2A1 CK2A1 protein protein human Homo sapiens Human MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ DIP protein P68400 original sequence version: 88 DIP protein P68400 original sequence version: 78 DIP protein P68400 original sequence version: 100 DIP protein P68400 original sequence version: 110 DIP protein P68400 original sequence version: 125 DIP protein P68400 original sequence version: 136 D3B6F5D13FF7422D q2v2m9-4 FH1/FH2 domain-containing protein 3 FHOD3 FHOS2 Formactin-2 Formin homolog overexpressed in spleen 2 KIAA1695 protein protein human Homo sapiens Human MATLACRVQFLDDTDPFNSTNFPEPSRPPLFTFREDLALGTQLAGVHRLLQAPHKLDDCTLQLSHNGAYLDLEATLAEQRDELEGFQDDAGRGKKHSIILRTQLSVRVHACIEKLYNSSGRDLRRALFSLKQIFQDDKDLVHEFVVAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVINRNETIQWLYTLIGSKFRLVVKTALKLLLVFVEYSESNAPLLIQAVTAVDTKRGVKPWSNIMEILEEKDGVDTELLVYAMTLVNKTLSGLPDQDTFYDVVDCLEELGIAAVSQRHLNKKGTDLDLVEQLNIYEVALRHEDGDETTEPPPSGCRDRRRASVCSSGGGEHRGLDRRRSRRHSVQSIKSTLSAPTSPCSQSAPSFKPNQVRDLREKEEEEEEEQPITEPSSEEEREDDASCQGKDSKVGAASGQSPTGRDAAPKSSALPAVSNASSQGKPLLVGTAGGTTWHSGSSGSEATPSALLSPPASAARPSSATPGSLKVSPTIDKLPYVPHSPFHLFSYDFEDSSLSTKEKEAESQKENSSSDSFSLSTYSASEPYHFRSFSSNRYSNFGNNSYHSSRPSSGSSVPTTPTSSVSPPQEARLERSSPSGLLTSSFRQHQESLAAERERRRQEREERLQRIEREERNKFSRDYLDKREEQRQAREERYKYLEQLAAEEHEKELRSRSVSRGRADLSLDLTSPAAPACLAPLSHSPSSSDSQEALTVSASSPGTPHHPQASAGDPEPESEAEPEAEAGAGQVADEAGQDIASAHEGAETEVEQALEQEPEERASLSEKERQNEGVNERDNCSASSVSSSSSTLEREEKEDKLSRDRTTGLWPAGVQDAGVNGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQANSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQPKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHREAPGPPPPPPPTFLGLPPPPPPPLLDSIPPPPVPGNLLVPPPPVFNAPQGLGWSQVPRGQPTFTKKKKTIRLFWNEVRPFDWPCKNNRRCREFLWSKLEPIKVDTSRLEHLFESKSKELSVSKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKIAILNFDEYALNKEGIEKILTMIPTDEEKQKIQEAQLANPEIPLGSAEQFLLTLSSISELSARLHLWAFKMDYETTEKEVAEPLLDLKEGIDQLENNKTLGFILSTLLAIGNFLNGTNAKAFELSYLEKVPEVKDTVHKQSLLHHVCTMVVENFPDSSDLYSEIGAITRSAKVDFDQLQDNLCQMERRCKASWDHLKAIAKHEMKPVLKQRMSEFLKDCAERIIILKIVHRRIINRFHSFLLFMGHPPYAIREVNINKFCRIISEFALEYRTTRERVLQQKQKRANHRERNKTRGKMITDTDEEEEVESGKFSGSSPAPPSQPQGLSYAEDAAEHENMKAVLKTSSPSVEDATPALGVRTRSRASRGSTSSWTMGTDDSPNVTDDAADEIMDRIVKSATQVPSQRVVPRERKRSRANRKSLRRTLKSGLTPEEARALGLVGTSELQL This existing Trembl entry is due to be integrated into an existing UniProt entry, visible in January 2013 -as Q2V2M9-4. Contains a phosphothreonine at position 1474. Contains a phosphothreonine at position 1476. EFCFEA5D7FF75299 fhod3_human FH1/FH2 domain-containing protein 3 FHOD3 FHOS2 KIAA1695 Formactin-2 Formin homolog overexpressed in spleen 2 protein protein human Homo sapiens Human MATLACRVQFLDDTDPFNSTNFPEPSRPPLFTFREDLALGTQLAGVHRLLQAPHKLDDCTLQLSHNGAYLDLEATLAEQRDELEGFQDDAGRGKKHSIILRTQLSVRVHACIEKLYNSSGRDLRRALFSLKQIFQDDKDLVHEFVVAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVINRNETIQWLYTLIGSKFRLVVKTALKLLLVFVEYSESNAPLLIQAVTAVDTKRGVKPWSNIMEILEEKDGVDTELLVYAMTLVNKTLSGLPDQDTFYDVVDCLEELGIAAVSQRHLNKKGTDLDLVEQLNIYEVALRHEDGDETTEPPPSGCRDRRRASVCSSGGGEHRGLDRRRSRRHSVQSIKSTLSAPTSPCSQSAPSFKPNQVRDLREKYSNFGNNSYHSSRPSSGSSVPTTPTSSVSPPQEARLERSSPSGLLTSSFRQHQESLAAERERRRQEREERLQRIEREERNKFRYKYLEQLAAEEHEKELRSRSVSRGRADLSLDLTSPAAPACLAPLSHSPSSSDSQEALTVSASSPGTPHHPQASAGDPEPESEAEPEAEAGAGQVADEAGQDIASAHEGAETEVEQALEQEPEERASLSEKERQNEGVNERDNCSASSVSSSSSTLEREEKEDKLSRDRTTGLWPAGVQDAGVNGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQANSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQPKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHREAPGPPPPPPPTFLGLPPPPPPPLLDSIPPPPVPGNLLVPPPPVFNAPQGLGWSQVPRGQPTFTKKKKTIRLFWNEVRPFDWPCKNNRRCREFLWSKLEPIKVDTSRLEHLFESKSKELSVSKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKIAILNFDEYALNKEGIEKILTMIPTDEEKQKIQEAQLANPEIPLGSAEQFLLTLSSISELSARLHLWAFKMDYETTEKEVAEPLLDLKEGIDQLENNKTLGFILSTLLAIGNFLNGTNAKAFELSYLEKVPEVKDTVHKQSLLHHVCTMVVENFPDSSDLYSEIGAITRSAKVDFDQLQDNLCQMERRCKASWDHLKAIAKHEMKPVLKQRMSEFLKDCAERIIILKIVHRRIINRFHSFLLFMGHPPYAIREVNINKFCRIISEFALEYRTTRERVLQQKQKRANHRERNKTRGKMITDSGKFSGSSPAPPSQPQGLSYAEDAAEHENMKAVLKTSSPSVEDATPALGVRTRSRASRGSTSSWTMGTDDSPNVTDDAADEIMDRIVKSATQVPSQRVVPRERKRSRANRKSLRRTLKSGLTPEEARALGLVGTSELQL 7CE7A8C0054856BE sqstm_rat Sequestosome-1 Sqstm1 Zip Protein kinase C-zeta-interacting protein Ubiquitin-binding protein p62 protein protein rat Rattus norvegicus (Rat) Rat MASLTVKAYLLGKEEAAREIRRFSFCFSPEPEAEAAAGPGPCERLLSRVAVLFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEARSMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPSEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL E611F296E2B11464 sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD fhod3_mouse FH1/FH2 domain-containing protein 3 Fhod3 Fhos2 Kiaa1695 Formin homolog overexpressed in spleen 2 protein protein mouse Mus musculus Mouse MATLACRVQFLDDTDPFNSTNFPEPSRPPLFTFREDLALGTQLAGVHRLLRAPHKLDDCTLQLSHNGAYLDLEATLAEQRDELEGFQDDTGRGKKNSIILRTQLSVRVHACIEKLYNSSGRDLRRALFSLKQIFQDDKDLVHEFVIAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVINHSETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAPLLIQAVSAVDTKRGVKPWSNIMEILEEKDGVDTELLVYAMTLVNKTLAGLPDQDTFYDVVDCLEELGIAAVSQRHLNKKGTDLDLLEQFNIYEVALRHEDGDETAEPPPSGHRDRRRASMCSGGTVGEQQGLDRRRSRRHSIQNIKSPLSAPTSPCSPSVPAFKPSQVRDLCEKDEEEEEEEEQPITEPNSEEEREDDAQCQGKDSKASSASGQSSPGKDAAPESSALHTTSSPTSQGRWLSASTAARSPVLGGTSGPEASRPAARLLPPSPGLATRPSTAPKVSPTIDKLPYVPHSPFHLFSYDFEDSPLLTKDKGGDSQTENRYSNFSSNSFQSSRPSPGPSGSPSYASSFSSPQDTRSSPSGLLTSSFRQHQESLAAERERRRQEREERLQRIEREERNKFNREYLDKREEQRQARGERYKYLEQLAAETQEKEPRSQSVSRGRADLSLDLSLPAAPAPPSPSSQSPSADSQEALPVPSSPPTLQCPQVSGKDHEPELEAEAGQGADEASQDIASAHRGAESQEEPVLELEPEERASLSEKERQNEEVNERDNCSASSISSSSSTLEREEKEDKLSEDRATGLWSTSLQDVGVNGQCGDILTSKRFMLDMLYAHNRKSTEDEEKDDGEPGRSAQEVEAVASLATRISTLQANSQAPEESIKRVDIGCLDNRGSVKAFAEKFNSGEVGRGAISPDVESQDKVPDTPPAQLKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHREAPGPPPPPPPTFLGLPPPPPPPLLDSVPPPPVPGNLLASPVFNTPQGLGWSQVPRGQPAFTKKKKTIRLFWNEVRPFEWPSKNNRRCREFLWSKLEPIKVDTSRLEHLFESKSKELSVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKIAILNFDEYALNKEGIEKILTMIPTEEEKQKIQEAQLANPEVPLGSAEQFLLTLSSISELSARLHLWAFKMDYETTEKEVAEPLLDLKEGIDQLENNKTLGFILSTLLAIGNFLNGTNAKAFELSYLEKVPEVKDTVHKQSLLHHVCTMVVENFPDSSDLYSEIGAITRSAKVDFDQLQDNLCQMERRCKASWDHLKAIAKHEMKPVLKQRMSEFLKDCAERIIILKIVHRRIINRFHSFLLFMGHPPYAIREVNINKFCRIISEFALEYRTTRERVLQQKQKRANHRERNKTRGKMITDSGKFSGSSPAAPSQPQGLSYAEDAAEHENMKAVLKTSSPALEDATPVLGVRTRSRASRGSTSSWTMGTEESPSVTDDAADEIMDRIVKSATQVPSQRVVPRERKRSRANRKSLRRTLKSGLTPEEARALGLVGTSELQL F9EE9B7354D51A70 csnk2a1-fhod3 5 11 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) 12 enzyme target enzyme target substrate substrate neutral component neutral component fh2_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q2V2M9-4:p.Thr1476Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A Wed May 02 18:24:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1476ala' Tue Jun 30 16:36:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1476Ala' Q2V2M9-4:p.[Thr1474Ala;Thr1476Ala] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:39:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1474ala,thr1476ala' Tue Jun 30 17:58:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1474Ala;Thr1476Ala]' Q2V2M9-4:p.Thr1474Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T A Wed May 02 20:25:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1474ala' Tue Jun 30 17:54:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1474Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1C Phosphorylation by CK2 was detected only in FHOD3-FH2 domain constructs that contained the alternative exon (FH2L) but not in constructs lacking it (FH2S). fhod3-sqstm1 1 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait fh2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association None fhod3-sqstm1-1 6 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait fd2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3A fhod3-sqstm1-2 6 12 unspecified role unspecified role bait bait fh2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role prey prey physical association physical association 3A-2 Weak reaction sqstm1-fhod3 3 13 unspecified role unspecified role prey prey purified purified fh2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 3B sqstm1-fhod3-1 3 12 unspecified role unspecified role prey prey purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 3B Phosphorylation of FH2L-GST reduces binding. q2v2m9_4-sqstm1 8 15 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait purified purified region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q2V2M9-4:p.[Thr1474Asp;Thr1476Asp] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T D certain certain sequence position certain certain certain sequence position certain T D Wed May 02 18:45:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1474asp,thr1476asp' Tue Jun 30 20:05:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr1474Asp;Thr1476Asp]' physical association physical association 3C Phosphorylation of FH2L-GST reduces binding. sqstm1-fhod3-2 8 13 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pb1_domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 3C/D/E q2v2m9_4-sqstm1-1 7 15 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component purified purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3F Differential FHOD3 distribution was observed, depending on whether FHOD3 contained or lacked the T(D/E)5XE exon; colocalisation in cytoplasmic bodies was only seen with p62 and the form of FHOD3 lacking the T(D/E)5XE exon. fhod3-sqstm1-3 7 13 unspecified role unspecified role neutral component neutral component purified purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3F fhod3-sqstm1-4 7 14 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component purified purified ha_or_gfp_tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3G No colocalization could be detected with T(D/E)5XE exon–containing FHOD3. fhod3-sqstm1-5 7 14 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization 3H DMAT-a CK2 Inhibitor fhod3-sqstm1-6 2 15 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component purified purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 4 fhod3-sqstm1-7 4 10 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component purified purified colocalization colocalization 6C