bhf-ucl Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. 293T cells were transfected with HA-tagged constructs and GST-fusion proteins. Lysates were precipitated with glutathione beads, and analyzed for bound HA-tagged proteins using anti-HA. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. Active Mek2 as a regulatory scaffold that promotes Pin1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell migration. J. Cell. Sci. (0021-9533) 2010 imex curation Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pan C.Q., Liou Y.C., Low B.C. Cardiac - Interactions involving cardiac related proteins This paper is complicated. I had a hard time trying to figure out how to annotate all the different combinations of interactions that they show. Pull downs in figures 2F and 2G couldn't be annotated because the authors do not provide the species origin of Rhotekin Rho binding domain. dbslowbc@nus.edu.sg Accepted 2014-NOV-03 by PPORRAS J. Cell. Sci. (0021-9533) 2010 Only protein-protein interactions imex curation This one is indeed complicated. Maybe we can go through it together when you are back, there's too much stuff to re-curate. pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 p85298-4 Rho GTPase-activating protein 8 ARHGAP8 Rho-type GTPase-activating protein 8 protein protein human Homo sapiens Human MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTPEAPGEHGLAPWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL No experimental confirmation available. 0AE4B430EAEBF0D3 mp2k2_mouse Dual specificity mitogen-activated protein kinase kinase 2 Map2k2 Mek2 Mkk2 Prkmk2 ERK activator kinase 2 MAPK/ERK kinase 2 protein protein mouse Mus musculus Mouse MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELDLDEQQRKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKARHRPSGLIMARKLIHLEIKPAVRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEDILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVELAIGRYPIPPPDAKELEASFGRPVVDGADGEPHSVSPRPRPPGRPISVGHGMDSRPAMAIFELLDYIVNEPPPKLPSGVFSSDFQEFVNKCLIKNPAERADLKLLMNHAFIKRSEGEEVDFAGWLCRTLRLKQPSTPTRTAV F00CD807E86F5B26 mp2k1_mouse Dual specificity mitogen-activated protein kinase kinase 1 Map2k1 Mek1 Prkmk1 ERK activator kinase 1 MAPK/ERK kinase 1 protein protein mouse Mus musculus Mouse MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCHVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI 01D1D18572AE40E7 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 pin1-arhgap8-5 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:18:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 19:49:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' Q63932:p.Lys101Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:22:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys101ala' Tue Jun 30 18:41:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys101Ala' association association Fig. 3A, 3E, 4A, 4B, 4C, 5E, S4A, 5F, 5G Mek2-SD (mutant S222D-S226D) enhances the interaction between Flag-Pin 1 and HA-PC. As the expressed amount of Mek2-SD increases, the interaction between PC and Pin1 (in the triple complex) decreases (Fig. 4A) As the expressed amount of Mek2-K (mutant K101A) increases, the interaction between PC and Pin1 is disrupted. As the expressed amount of Mek2 WT increases the interaction of Mek2WT with the complex PC-Pin1 appears (1.5ug of plasmid) (Fig. 4B) CIAP treatment improved the interaction between Mek2-SD and the PC-PIN1 complex (Fig. S4A) The loss of the following PC fragment: 256-260aa prevents Mek2-SD from binding to Pin1 and reduces the binding between Pin1 and BPGAP1 PC fragment pin1-arhgap8 3 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P85298-4:p.[Asp256Lys;Asp257Lys] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain D K Wed May 02 20:02:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp256lys,asp257lys' Tue Jun 30 20:01:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp256Lys;Asp257Lys]' 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1D, 1I, 5A 256-260aa PC deletion (PC3D) decreases its interaction with WW as shown in Fig. 1I arhgap8-map2k2-6 4 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Ala;Ser226Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:49:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222ala,ser226ala' Tue Jun 30 20:07:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Ala;Ser226Ala]' Q63932:p.Lys101Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:27:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys101ala' Tue Jun 30 15:43:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys101Ala' physical association physical association Fig. 3D bpgap1-pin1-1 6 8 unspecified role unspecified role bait bait pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association Fig. 3C Interaction between PC and endogenous Pin1increases when Mek2-SD is expressed. pin1-arhgap8-23 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P31938:p.[Ser222Asp;Thr226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain T D Wed May 02 14:35:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,thr226asp' Tue Jun 30 16:06:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Thr226Asp]' association association Fig S3B pin1-map2k1 4 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P31938:p.[Ser218Asp;Ser222Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:31:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser218asp,ser222asp' Tue Jun 30 15:46:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser218Asp;Ser222Asp]' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig.S3C arhgap8-pin1-1 1 8 unspecified role unspecified role neutral component neutral component pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc3d sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain np sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4F Full length HA-BPGAP1 doesn't colocalize in the nucleus with Pin 1 arhgap8-map2k2-9 1 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Fri Jul 22 11:07:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser226asp,ser222asp' Wed May 02 19:13:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 19:51:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 8 unspecified role unspecified role neutral component neutral component pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined np sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 4E pin1-arhgap8-27 3 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:29:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 16:10:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain association association Fig. 5A, 5C, 5D, S4C Interaction between ww domain (of Pin1) and Mek2-SD is abolished when the PC domain of BPGAP1 deletes specific region: 176-189 (see constructions P1, P2, P3) CIAP treatment doesn't have any effect in the interaction among Mek2-SD, PC, and WW pin1-arhgap8-2 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rho gap domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B, 1F, 4A, 4B, 4C, 5F, S5 pin1-arhgap8-4 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey P04049:p.Tyr340Asp mutation mutation certain certain sequence position certain certain certain sequence position certain Y D Wed May 02 15:22:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr340asp' Tue Jun 30 19:19:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr340Asp' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig.3A HA-MeK2-K (dead mutant K101A) reduces the interaction between Flag Pin 1 and HA-PC. HA-active c-Raf (mutant Y340D) increases interaction between Flag -Pin1 and HA-PC pin1-arhgap8-6 4 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait Q13526:p.His157Ala mutation mutation certain certain sequence position certain certain certain sequence position certain H A Wed May 02 15:45:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his157ala' Tue Jun 30 17:29:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His157Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ppi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1J pin1-arhgap8-7 4 7 unspecified role unspecified role bait bait Q13526:p.Cys113Ala mutation mutation certain certain sequence position certain certain certain sequence position certain C A Wed May 02 14:25:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys113ala' Tue Jun 30 18:43:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys113Ala' ppi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc23 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1G arhgap8-map2k2-4 4 8 unspecified role unspecified role bait bait pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:57:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 17:39:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 7 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey association association Fig. 3C pin1-arhgap8-8 4 10 unspecified role unspecified role prey prey P31938:p.[Ser218Asp;Ser222Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:19:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser218asp,ser222asp' Tue Jun 30 20:19:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser218Asp;Ser222Asp]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Fig.S3C pin1-arhgap8-9 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig.S3C pin1-arhgap8-10 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:42:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 19:27:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3E, 4A pin1-arhgap8-12 4 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 167-175, 190-433 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5E pin1-arhgap8-13 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 167-179, 190-433 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5E pin1-arhgap8-16 4 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:18:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 18:32:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 167-175, 190-433 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5E pin1-arhgap8-20 4 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P85298-4:p.[Pro186Ala;Pro187Ala;Leu188Ala;Pro189Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:27:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro186ala,pro187ala,leu188ala,pro189ala' Tue Jun 30 18:48:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro186Ala;Pro187Ala;Leu188Ala;Pro189Ala]' P85298-4:p.[Pro184Ala;Pro186Ala] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Fri Jul 22 11:10:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro186ala,pro184ala' Wed May 02 18:38:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro184ala,pro186ala' Tue Jun 30 18:37:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro184Ala;Pro186Ala]' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5F arhgap8-map2k2-7 4 9 unspecified role unspecified role prey prey Q63932:p.Lys101Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:33:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys101ala' Tue Jun 30 17:14:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys101Ala' Q63932:p.[Ser222Ala;Ser226Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:01:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222ala,ser226ala' Tue Jun 30 20:11:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Ala;Ser226Ala]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:06:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 19:22:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 8 unspecified role unspecified role bait bait 167-255, 261-433 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5H pin1-arhgap8-24 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S3B pin1-map2k1-1 5 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P31938:p.[Ser222Asp;Thr226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain T D certain certain sequence position certain certain certain sequence position certain S D Fri Jul 22 11:05:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr226asp,ser222asp' Wed May 02 14:51:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,thr226asp' Tue Jun 30 15:54:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Thr226Asp]' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. S3B pin1-arhgap8-25 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q13526:p.His157Ala mutation mutation certain certain sequence position certain certain certain sequence position certain H A Wed May 02 15:11:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his157ala' Tue Jun 30 18:34:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His157Ala' 8 unspecified role unspecified role prey prey pc sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:50:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 20:00:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' association association Fig. S4B CIP treatment didn't have any effect in this interaction pin1-arhgap8-26 3 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ppi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P85298-4:p.[Asp256Lys;Asp257Lys] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain D K Wed May 02 19:54:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp256lys,asp257lys' Tue Jun 30 18:25:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp256Lys;Asp257Lys]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1D, 1I, 5A pin1-arhgap8-28 3 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 180-189 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait ww domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5C Interaction between ww domain (of Pin1) and Mek2-SD is abolished when the PC domain of BPGAP1 deletes specific region: 176-189 (see constructions P1, P2, P3) pin1-arhgap8-29 3 9 unspecified role unspecified role prey prey Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:43:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 18:14:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ww domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5D The PPLP mutant doesn't bind to WW and Mek2-SD arhgap8-map2k2-10 1 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Fri Jul 22 11:11:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser226asp,ser222asp' Wed May 02 16:54:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 19:17:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' colocalization colocalization Fig. 4E pin1-arhgap8-30 6 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3B pin1-arhgap8-33 6 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:18:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Wed May 02 18:21:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 18:09:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3B Mek2-SD increases the interaction between BPGAP1 (FL and PC) and Pin1. pin1-arhgap8-34 6 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 14:29:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 17:52:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' physical association physical association Fig. 3B When NNP (from BPGAP1) is expressed in the cell, Pin1is able to precipitate Mek2-SD but not NNP. pin1-arhgap8-35 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc23 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. S5 pin1-arhgap8-38 4 9 unspecified role unspecified role prey prey Q63932:p.[Ser222Asp;Ser226Asp] mutation mutation certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Wed May 02 19:49:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser222asp,ser226asp' Tue Jun 30 18:52:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser222Asp;Ser226Asp]' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey pc23 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pc-12 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Fig. S5 pin1-map2k2 4 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q63932:p.Lys101Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:52:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys101ala' Tue Jun 30 16:23:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys101Ala' physical association physical association Fig. 4C Figure 4C has a very weak band showing interaction between Pin1 and Mek2-K dead mutant. Figure 3E, doesn't show interaction between the mutant Mek2-K and Pin1.